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DNA fingerprinting based on SSR amplification profiles for Piper species identification (Piperaceae) Acta Botanica
Christ,Jheniffer Abeldt; Hollunder,Renan Köpp; Carvalho,Marina Santos; Ferreira,Marcia Flores da Silva; Garbin,Mário Luís; Carrijo,Tatiana Tavares.
ABSTRACT DNA fingerprinting based on SSR amplification profiles was applied to native species of Piper from the Atlantic Forest to compare the utility of this type of molecular marker with the morphological characters traditionally applied in Piper taxonomy and identification. Fifty-one SSR markers developed for four species of Piper native to Asia and Mesoamerica were applied to 16 species, together with 63 morphological characters, for species characterization. Molecular and morphological data were analysed by cluster analysis, followed by a cluster sharpness test and the construction of a heat map to visualize the association of characters with species groups. A multivariate regression tree determined the number of loci needed for species...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heat map; Microsatellites; Morphology; Multivariate analyses; Taxonomy.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000400511
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