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| Registros recuperados: 23 | |
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| Zhao,Youyun; Wu,Jianhua; Sun,Lijun; Liu,Guangzhong; Li,Bo; Zheng,Yi; Li,Xiaodong; Tao,Junxiu. |
| Abstract Objective There are a lot of disagreements in the studies on hepatitis B virus (HBV) DNA polymerase mutation rate associated with nucleos(t)ide analogues (NAs) in treatment-naive chronic hepatitis B (CHB) patients. This is the first study aimed to investigate the prevalence of spontaneous HBV resistance mutations in Central China. Methods This study included treatment-naive patients with CHB from June 2012 to May 2015 receiving care at the Institute of Liver Disease in Central China. All patients completed a questionnaire covering different aspects, such as family medical history, course of liver disease, medication history, alcohol use, among others. Mutations in HBV DNA polymerase associated with NAs resistance were detected using INNO-LiPA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatitis B virus; Chronic hepatitis B; Viral resistance mutations. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000200173 |
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| Alireza,Khabiri; Mohsen,Abolhassani; Sudabeh,Aslani; Hiedeh,Darabi; Farideh,Bagheri; Mohammad-Hossein,Alimohammadian. |
| In this report we have analysed the peripheral blood lymphocyte of several patients with chronic hepatitis B virus infection with flow cytometry. Based on the presence and absence of the HBeAb, patients were divided into two groups. In both, all the patients were HBsAg positive with normal range of serum alanine aminotranferase (23.9 ± 17.8). We have found that the immunophenotypic profiles of patients were different from healthy donors with significant decrease in CD3+ T cells, specially CD8+ T cells and a siginificant increase in the CD19+ B cells. The differences were seen in other subset of T cells (CD4+) or NK cells (CD56+/CD16+ ) and HLA-DR markers were not significant. When the phenotypic profiles of both groups were compared with each other, such... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatitis B virus; Chronic HBV infection; HBsAg; HBeAg; HBeAb; Lymphocyte subset; Flow cytometry. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000600009 |
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| Zhang,Jingmin; Wang,Yafeng; Peng,Youmei; Qin,Chongzhen; Liu,Yixian; Li,Jingjing; Jiang,Jinhua; Zhou,Yubing; Chang,Junbiao; Wang,Qingduan. |
| ABSTRACT Antiviral drug resistance is the most important factor contributing to treatment failure using nucleos(t)ide analogs such as lamivudine for chronic infection with hepatitis B virus (HBV). Development of a system supporting efficient replication of clinically resistant HBV strains is imperative, and new antiviral drugs are needed urgently to prevent selection of drug-resistant HBV mutants. A novel fluorinated cytidine analog, NCC (N-cyclopropyl-4′-azido-2′-deoxy-2′-fluoro-β-d-cytidine), was recently shown to strongly inhibit human HBV in vitro and in vivo. This study was designed to evaluate the antiviral activity of NCC against lamivudine-resistant HBV. We generated a stable cell line encoding the major pattern of lamivudine-resistant mutations... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: N-cyclopropyl-4′-azido-2′-deoxy-2′-fluoro-β-D- cytidine; Hepatitis B virus; HepG2.RL1 cells; Lamivudine-resistant; RtL180M/M204V. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000600477 |
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| Wang,Yong-Zhong; Zhu,Zhen; Zhang,Hong-Yu; Zhu,Min-Zhi; Xu,Xin; Chen,Chun-Hua; Liu,Long-Gen. |
| OBJECTIVE: To study the role of hepatitis B virus with A1762T/G1764A double mutation in liver cirrhosis and hepatocellular carcinoma, and create a sensitive, fast, accurate assay for detection of A1762T/G1764A double mutation.METHODS: We developed an accurate and fast real-time amplification refractory mutation system to detect A1762T/G1764A double mutation. Cloned hepatitis B virus genome was used as a control. Assay sensitivity was determined by serial dilution and mixed template experiments. Specificity was determined by cross experiments with wild and mutant hepatitis B virus. Fifty clinical samples were tested by the real-time amplification refractory mutation system and the results were compared with sequencing.RESULTS: The real-time amplification... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatitis B virus; Basal core promoter mutations; Amplification refractory mutation system; PCR. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300261 |
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| Almeida,Delvone; Tavares-Neto,José; Trepo,Christian; Almeida,Alessandro; Mello,Caroline; Chemin,Isabelle; Paraná,Raymundo. |
| A sentinel study on viral hepatitis is currently being carried out in the village of Cavunge in a semiarid rural region of the state of Bahia, northeastern Brazil. This study has identified individuals in whom anti-HBc IgG was the only serological marker for hepatitis B virus (HBV). This serological pattern may constitute evidence of occult HBV infection. This study Investigated the possibility of occult hepatitis B virus infection in individuals in a rural community who tested positive for anti-HBc IgG alone. A cross-sectional population-based study. ELISA III was performed on serum samples to test for serological viral markers, and ultrasensitive PCR (US-PCR) was used to assess viremia. Among the 1,536 serum samples, 3.6% (n=55) were positive for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anti-HBc alone; Hepatitis B virus; Occult hepatitis B; Ultrasensitive PCR. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400010 |
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| Araujo,Natalia M.; Vianna,Carlos O. A.; Moraes,Marcia T. B.; Gomes,Selma A.. |
| The impact of hepatitis B virus (HBV) genotypes on the sensitivity of surface antigen (HBsAg) detection assays has been poorly investigated. Here, plasmids carrying consensus or variant coding sequences for HBV surface proteins from genotypes A, D and F, were constructed. HBsAg levels were evaluated in medium and extracts of transfected CHO cells by a commercial polyclonal-based assay. We show that HBsAg detection values of consensus forms from genotypes D and F were, respectively, 37% and 30% lower than those obtained by genotype A. However, the presence of two single variations, T143M in genotype A, and T125M in genotype D, produced a decrease of 44% and an increase of 34%, respectively, on HBsAg mean values in comparison with their consensus forms. In... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatitis B virus; Surface antigen; Amino acid variations; Transfection. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000400005 |
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| Chachá,Silvana Gama Florencio; Gomes-Gouvêa,Michele Soares; Malta,Fernanda de Mello; Ferreira,Sandro da Costa; Villanova,Márcia Guimarães; Souza,Fernanda Fernandes; Teixeira,Andreza Correa; Passos,Afonso Dinis da Costa; Pinho,João Renato Rebello; Martinelli,Ana de Lourdes Candolo. |
| Abstract Hepatitis B virus (HBV) is distributed worldwide, with geographical variations regarding prevalence of the different genotypes. The aim of this study was to determine the HBV genotypes and subgenotypes circulating in Southeast Brazil and compare the genetic sequences found with HBV sequences previously described in the world. Sequences from 166 chronic HBV carriers were analyzed using the fragment constituted by 1306 base pairs comprising surface and polymerase regions of the HBV genome. The sequences obtained were submitted to phylogenetic analysis. HBV subgenotypes A1, A2, D1-D4, F2a, and F4 were found. HBV genotype D was the most frequent, found in 99 patients (58.4%). Within this group, subgenotype D3 was the most prevalent, in 73 patients... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatitis B virus; Genotype; Phylogenetics; Intra-familial transmission. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000400424 |
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| Moraes,Tatiane Cinquini; Fiaccadori,Fabíola Souza; Souza,Menira; Almeida,Tâmera Nunes Vieira; Cunha,Marielton dos Passos; Castro,Ítalo de Araújo; Cardoso,Divina das Dôres de Paula. |
| ABSTRACT OBJECTIVES: The main objective was to evaluate HBV infection and occult HBV infection (OBI) cases in mentally ill patients based on serological and molecular profiles. MATERIAL AND METHODS: Serum samples of 333 long-stay mentally ill patients were tested for the prevalence of HBV markers by serological (ELISA) and molecular (PCR) assays. The PCR products were sequenced to determine viral genotypes. RESULTS: It was observed a global prevalence of 12.9% (43/333) for HBV infection markers, considering HBsAg and/or anti-HBc positivity. Fourteen samples tested positive for anti-HBs alone. All samples positive (n= 57) for any HBV serological markers were tested for HBV-DNA and six were positive: HBsAg/anti-HBc (n = 1), anti-HBc/anti-HBs (n = 1),... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Hepatitis B virus; Occult infection; Prevalence; Mental illness. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000600643 |
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| Ferrari,T.C.A.; Xavier,M.A.P.; Vidigal,P.V.T.; Amaral,N.S.; Diniz,P.A.; Resende,A.P.; Miranda,D.M.; Faria,A.C.; Lima,A.S.; Faria,L.C.. |
| Estimates of occult hepatitis B virus (HBV) infection prevalence varies among different studies depending on the prevalence of HBV infection in the study population and on the sensitivity of the assay used to detect HBV DNA. We investigated the prevalence of occult HBV infection in cirrhotic patients undergoing liver transplantation in a Brazilian referral center. Frozen liver samples from 68 adults were analyzed using a nested polymerase chain reaction assay for HBV DNA. The specificity of the amplified HBV sequences was confirmed by direct sequencing of the amplicons. The patient population comprised 49 (72.1%) males and 19 (27.9%) females with a median age of 53 years (range=18-67 years). Occult HBV infection was diagnosed in three (4.4%) patients. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Occult hepatitis B virus infection; Hepatitis B virus; HBV DNA; Liver transplantation. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001100990 |
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| Wang,Ke; Pei,Hao; Huang,Biao; Yang,Run-Lin; Wu,Hang-Yuan; Zhu,Xue; Zhu,Lan. |
| The role of B cells in the pathogenesis of hepatitis B virus (HBV) infection has not been explored in depth. In the present study, the activation status of B cells from peripheral blood of healthy controls (N = 20) and patients with acute hepatitis B (AHB, N = 15) or chronic hepatitis B (CHB, N = 30) was evaluated by measuring the expression levels of B-cell activation markers CD69 and CD86, using quantitative real-time PCR and flow cytometry. Moreover, the potential mechanism underlying B-cell activation during HBV infection was further investigated by analyzing the expression profile of FCRL1, an intrinsic activation molecule of B cells. An elevation in the levels of B-cell activation markers including CD69 and CD86 was observed in the AHB patients... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatitis B virus; B-cell activation; Fc receptor-like 1; Gene expression. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200002 |
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| Bertolini,D.A.; Pinho,J.R.R.; Saraceni,C.P.; Moreira,R.C.; Granato,C.F.H.; Carrilho,F.J.. |
| The prevalence of hepatitis B virus (HBV) in Brazil increases from South to North but moderate to elevated prevalence has been detected in the Southwest of Paraná State. The prevalence of serological markers of HBV was evaluated in 3188 pregnant women from different counties in Paraná State and relevant epidemiological features were described. The prevalence of HBV markers in pregnant women for the state as a whole was 18.5% (95% CI = 17.2-19.9), ranging from 7.2% in Curitiba to 38.5% in Francisco Beltrão. The endemicity of HBV marker prevalence in pregnant women was intermediate in Cascavel, Foz do Iguaçu, and Francisco Beltrão, and low in Curitiba, Londrina, Maringá, and Paranaguá. Multiple logistic regression showed that HBV marker prevalence increased... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatitis B virus; Brazil; Serological markers; Pregnant women. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800011 |
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| Chen,Y.M.; Wu,S.H.; Qiu,C.N.; Yu,D.J.; Wang,X.J.. |
| The objective of this study was to examine hepatitis B virus (HBV) subgenotypes and mutations in enhancer II, basal core promoter, and precore regions of HBV in relation to risks of liver cirrhosis (LC) and hepatocellular carcinoma (HCC) in Southeast China. A case-control study was performed, including chronic hepatitis B (CHB; n=125), LC (n=120), and HCC (n=136). HBV was genotyped by multiplex polymerase chain reaction and subgenotyped by restriction fragment length polymorphism. HBV mutations were measured by DNA sequencing. HBV genotype C (68.2%) predominated and genotype B (30.2%) was the second most common. Of these, C2 (67.5%) was the most prevalent subgenotype, and B2 (30.2%) ranked second. Thirteen mutations with a frequency >5% were detected.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatitis B virus; Genotype; Core promoter; Precore; Mutation; Advanced liver disease. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000700614 |
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| França,P.H.C.; Coelho,H.S.M.; Brandão,C.E.; Segadas,J.A.; Quintaes,R.F.; Carrilho,F.J.; Ono-Nita,S.; Mattos,A.A.; Tovo,C.; Gouvea,V.S.; Sablon,E.; Vanderborght,B.O.M.. |
| Given the loss of therapeutic efficacy associated with the development of resistance to lamivudine (LMV) and the availability of new alternative treatments for chronic hepatitis B patients, early detection of viral genotypic resistance could allow the clinician to consider therapy modification before viral breakthrough and biochemical relapse occur. To this end, 28 LMV-treated patients (44 ± 12 years; 24 men), on their first therapy schedule, were monitored monthly at four Brazilian centers for the emergence of drug resistance using the reverse hybridization-based INNO-LiPA HBV DR assay and occasionally sequencing (two cases). Positive viral responses (HBV DNA clearance) after 6, 12, and 18 months of therapy were achieved by 57, 68, and 53% of patients,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatitis B; Antiviral therapy; Lamivudine; Drug resistance; YMDD variants; Hepatitis B virus. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200003 |
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| Registros recuperados: 23 | |
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