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Infections à virus de type herpes chez les bivalves : large spectre d'hôte et détection au stade adulte ArchiMer
Arzul, Isabelle; Renault, Tristan.
In 1972, Farley reported the presence of herpes-type viral particles in adult American oysters (Crassostrea virginica) for the first time. Since then, this pathogen has been detected in various marine bivalve species, usually associated with massive mortality events. To date, herpes-type viruses have been detected in the following species: C gigas, Ostrea edulis, O. angasi, Tiostrea chilensis, Ruditapes decussatus, R. philippinarum and most recently, Pecten maximus.
Tipo: Text Palavras-chave: Pathogènes; Bivalves; Herpesvirus; Herpesviridae.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/acte-3266.pdf
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Detection of oyster herpesvirus DNA and proteins in asymptomatic Crassostrea gigas adults ArchiMer
Arzul, Isabelle; Renault, Tristan; Thebault, Anne; Gerard, Andre.
Since 1972, several herpes-like virus infections have been reported among different bivalve species around the world. Most of these reports involved larvae or juveniles presenting high mortalities. Two case reports of herpes-like viruses concerned adult oysters, Crassostrea virginica in USA and Ostrea angasi in Australia. Molecular techniques including PCR and in situ hybridization (ISH) have been recently developed to detect the oyster herpesvirus genome. In the present study, 30 Pacific oyster, Crassostrea gigas, adults have been analyzed using three different techniques: PCR, ISH and immunochemistry, in order to detect herpesviruses in asymptomatic individuals. PCR and ISH allowed detection of oyster herpesvirus DNA in 93.3 and 86.6%, respectively, of...
Tipo: Text Palavras-chave: Protein; DNA; Healthy carrier; Adult oyster; Crassostrea gigas; Herpesvirus.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-697.pdf
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Shellfish viruses ArchiMer
Renault, Tristan.
Mortalities in a number of commercially important mollusc species have been associated with the detection of viruses belonging to several families. The first description of a virus was in adult eastern oyters, Crassostrea virginica, with the detection of virus particles resembling members of the family Herpesviridae. Subsequently, mass mortalities in French stocks of adult Portuguese oysters, C. angulata, were associated with irido-like virus infection. Other viruses observed in molluscs are described as members of the families Iridoviridae, Papovaviridae and Reoviridae. Little information is available on viral infections that affect molluscs due to primarily to the inadequacy of diagnostic methods that are employed when mass mortality events occur. Most...
Tipo: Text Palavras-chave: Virus; Scallops; Picornavirus; Oyter herpesvirus 1; Oysters; Molluscs; Irido Like virus; Diseases; Herpesvirus; Clams; Bivalves; Birnavirus; Abalones.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4932.pdf
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Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection ArchiMer
Corporeau, Charlotte; Tamayo, David; Pernet, Fabrice; Quere, Claudie; Madec, Stephanie.
Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 μVar) and to determine which metabolic pathways might be affected during infection. Animals were sampled for proteomic analysis two days post-injection, at the same time as OsHV-1 initiated an intense replication phase in challenged oysters. Twenty-five abundant protein spots that showed a marked change in accumulated levels were identified using a two-dimensional electrophoresis (2-DE) proteomic approach. Overall, these proteins are involved in cytoskeleton organization, protein turnover, induction of stress signals, signalling pathways and energy metabolism. Challenged oysters...
Tipo: Text Palavras-chave: Marine bivalves; Crassostrea gigas; Herpesvirus; Metabolism; Warburg.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00199/31035/29439.pdf
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The development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of abalone herpesvirus DNA ArchiMer
Chen, Meilin; Kuo, S. T.; Renault, Tristan; Chang, P. H..
A loop-mediated isothermal amplification (LAMP) assay was developed for the detection of abalone herpesvirus DNA. Two pairs of primers were designed, based on the sequence of the DNA polymerase gene of abalone herpesvirus. The reaction temperature and time were optimized to 63 °C and 60 min, respectively. LAMP amplicons were analyzed by 2% agarose gel electrophoresis or by visual inspection of a colour change emitted by fluorescent dye. The method developed was specific for the detection of abalone herpesvirus, without cross-reactions with other tested herpesviruses including ostreid herpesvirus 1 (OsHV-1), European eel herpesvirus, koi herpesvirus (KHV) and an avian herpesvirus. The LAMP assay was 100 folds more sensitive than a conventional PCR and 10...
Tipo: Text Palavras-chave: Herpesvirus; Abalone; Haliotis diversicolor supertexta; Polymerase chain reaction; Loop-mediated isothermal amplification; SYBR green PCR.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00166/27690/25882.pdf
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Contribution à l'étude de mollusques marins : Iridovirus-like et Herpèsvirus-like. Description, caractérisation biochimique, cycle de multiplication viral, diagnostic et étude épidémiologique ArchiMer
Renault, Tristan; Le Deuff, Rose-marie; Cochennec, Nathalie; Chollet, Bruno; Maffart, Patricia.
Among the marine molluscs, Pacifie oyster, Crassostrea gigas, is actually the most reared specie in the world. Noticely in France, where it was imported between 1960 and 1970, and replaced by Portuguese oyster, Crassostrea angulata, which specie was decimated during this period because of iridoviruses. Uncontroled introductions of new species may however be hazardous. Indeed, they might be responsible for the appearance and the dissemination of new diseases of bivalve molluscs in importing country. In addition to these observations, the quasi monobreeding of Pacific oyster in France enhances the necessity to develop research concerning the diagnosis of pathologies affecting this specie, namely the diagnosis of viral diseases. Several families of viruses...
Tipo: Text Palavras-chave: Mollusques; Crassostrea gigas; Pathologie; Iridovirus; Herpèsvirus; Diagnostic; Molluscs; Crassostrea gigas; Pathology; Iridovirus; Herpesvirus; Diagnosis.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00041/15230/12548.pdf
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Effects of temperature on herpes-like virus detection among hatchery-reared larval Pacific oyster Crassostrea gigas ArchiMer
Le Deuff, Rose-marie; Renault, Tristan; Gerard, Andre.
This paper describes effects of temperature on herpes-like virus detection in Pacific oyster Crassostrea gigas larvae held at different temperatures. Intranuclear, intracytoplasmic and extracellular viral particles were observed in velum and mantle connective tissues of oyster larvae reared at 25-26°C. In larvae held at 22-23OC, although nuclear lesions were observed, the presence of viral particles was not detected. Results were obtained for oyster larvae with 4 different parental origins. Herpesvirus infection was found in 3 of the 4 groups of oyster larvae held In the same conditions at the higher temperature.
Tipo: Text Palavras-chave: Temperature; Mortality; Larval culture; Crassotrea gigas; Pacific oyster; Herpesvirus.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/1996/publication-2900.pdf
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Evidence for interspecies transmission of oyster herpesvirus in marine bivalves ArchiMer
Arzul, Isabelle; Renault, Tristan; Lipart, Cécile; Davison, Andrew.
Since 1991, numerous herpesvirus infections associated with high mortality have been reported around the world in various marine bivalve species. In order to determine whether these infections are due to ostreid herpesvirus-l (OsHV1), a previously characterized pathogen of the Japanese oyster (Crassostrea gigas), PCR analysis was carried out on 30 samples of larvae collected from four bivalve species (C. gigas, Ostrea edulis, Ruditapes decussatus and Ruditapes philippinarum), most exhibiting mortality prior to collection. All samples were shown to be infected by OsHV1. Viral genomes in three samples of C. gigas and three of R. philippinarum that originated from the same hatchery were unusual in bearing a deletion of at least 2.8 kbp in an inverted repeat...
Tipo: Text Palavras-chave: Bivalve; Crassostrea gigas; Herpesvirus; Japanese oyster; Transmission.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-705.pdf
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Ontogeny and water temperature influences the antiviral response of the Pacific oyster, Crassostrea gigas ArchiMer
Green, Timothy; Montagnani, Caroline; Benkendorff, Kirsten; Robinson, Nick; Speck, Peter.
Disease is caused by a complex interaction between the pathogen, environment, and the physiological status of the host. Determining how host ontogeny interacts with water temperature to influence the antiviral response of the Pacific oysters, Crassostrea gigas, is a major goal in understanding why juvenile Pacific oysters are dying during summer as a result of the global emergence of a new genotype of the Ostreid herpesvirus, termed OsHV-1 μvar. We measured the effect of temperature (12 vs 22 °C) on the antiviral response of adult and juvenile C. gigas injected with poly I:C. Poly I:C up-regulated the expression of numerous immune genes, including TLR, MyD88, IκB-1, Rel, IRF, MDA5, STING, SOC, PKR, Viperin and Mpeg1. At 22 °C, these immune genes showed...
Tipo: Text Palavras-chave: Crassostrea; OsHV-1; Water temperature; Antiviral response; Herpesvirus.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00162/27318/25674.pdf
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Rapid and sensitive detection of ostreid herpesvirus 1 in oyster samples by real-time PCR ArchiMer
Pepin, Jean-francois; Riou, Antoine; Renault, Tristan.
Herpes and herpes-like virus infections have been reported in various marine mollusc species associated with high mortality rates. Following the characterisation and genome sequencing of ostreid herpesvirus 1 (OsHV-1), specific diagnostic tools have been developed based on conventional PCR techniques or in situ hybridisation. We have now developed a real-time PCR assay for rapid, sensitive and quantitative detection of OsHV-1, and compared it with a conventional PCR technique described previously. The new assay utilised SYBR® Green chemistry with specific primers C9/C10 targeting the C region. The melt curve analysis of OsHV-1 DNA or DNA extracted from infected material showed only one melting temperature peak (75.75 ± 0.1 °C). The assay had a detection...
Tipo: Text Palavras-chave: SYBR® Green; Real time PCR; Crassostrea gigas; Ostreid herpesvirus 1; Herpesvirus.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3951.pdf
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Detection of ostreid herpesvirus 1 DNA by PCR in bivalve molluscs: A critical review ArchiMer
Batista, Frederico; Arzul, Isabelle; Pepin, Jean-francois; Ruano, Francisco; Friedman, Carolyn S.; Boudry, Pierre; Renault, Tristan.
Herpes-like viral infections have been reported in different bivalve mollusc species throughout the world. High mortalities among hatchery-reared larvae and juveniles of different bivalve species have been associated often with such infections. The diagnosis of herpes-like viruses in bivalve molluscs has been performed traditionally by light and transmission electron microscopy. The genome sequencing of one of these viruses, oyster herpesvirus 1 (OsHV-1), allowed the development of DNA-based diagnostic techniques. The polymerase chain reaction (PCR) has been used for the detection of OsHV-1 DNA in bivalve molluscs at different development stages. In addition, the PCR used for detection of OsHV-1 has also allowed the amplification of DNA from an OsHV-1...
Tipo: Text Palavras-chave: Oyster; Bivalve molluscs; PCR; Detection; OsHV 1; Herpesvirus.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2322.pdf
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Complete genome sequence of acute viral necrosis virus associated with massive mortality outbreaks in the Chinese scallop, Chlamys farreri ArchiMer
Ren, Weicheng; Chen, Haixia; Renault, Tristan; Cai, Yuyong; Bai, Changming; Wang, Chongming; Huang, Jie.
Acute viral necrosis virus (AVNV) is the causative agent of a serious disease resulting in high mortality in cultured Chinese scallops, Chlamys farreri. We have sequenced and analyzed the complete genome of AVNV. The AVNV genome is a linear, double-stranded DNA molecule of 210,993 bp with a nucleotide composition of 38.5% G + C. A total of 123 open reading frames were predicted to encode functional proteins, ranging from 41 to 1,878 amino acid residues. The DNA sequence of AVNV is 97% identical to that of ostreid herpesvirus 1 (OsHV-1), and the amino acid sequences of the encoded proteins of these two viruses are 94-100% identical. The genomic organization of AVNV is similar to that of OsHV-1, and consists of two unique regions (170.4 kb and 3.4 kb,...
Tipo: Text Palavras-chave: Acute viral necrosis virus; Herpesvirus; OsHV-1; Genome; Scallop; Chlamys farreri; AVNV.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00135/24620/22650.pdf
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Contribution à l'étude de virus de mollusques marins apparentés aux Iridoviridae et aux Herpesviridae ArchiMer
Le Deuff, Rose-marie.
Among the marine molluscs, Pacific oyster, Crassostrea gigas, is actually the most reared species in the world. Noticeably in France, where it was imported between 1960 and 1970, and replaced Portuguese oyster, Crassostrea angulata. Intensification of breeding and the associated introductions of new species may be hazardous. Indeed, they might be responsible for the appearance and the dissemination of new diseases in bivalve molluscs. In addition to these observations, the quasi monobreeding of C. gigas in France enhances the necessity to develop research concerning the diagnosis of pathologies affecting this species, namely the diagnosis of viral diseases. Several families of viruses have been described within marine bivalves molluscs. The purpose of this...
Tipo: Text Palavras-chave: Mollusques bivalves; Crassostrea gigas; Huître japonaise; Pathologie; Iridovirus; Herpèsvirus; Diagnostic; Bivalve molluscs; Crassostrea gigas; Pacific oyster; Pathology; Iridovirus; Herpesvirus; Diagnosis.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00107/21792/19367.pdf
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Deciphering the effect of food availability, growth and host condition on disease susceptibility in a marine invertebrate ArchiMer
Pernet, Fabrice; Tamayo, David; Fuhrmann, Marine; Petton, Bruno.
Food provisioning influences disease risk and outcome in animal populations in two ways. On the one hand, unrestricted food supply improves the physiological condition of the host and lowers its susceptibility to infectious disease, reflecting a trade-off between immunity and other fitness-related functions. On the other hand, food scarcity limits the resources available to the pathogen and slows the growth and metabolism of the host on which the pathogen depends to proliferate. Here, we investigated how food availability, growth rate and energetic reserves drive the outcome of a viral disease affecting an ecologically relevant model host, the Pacific oyster, Crassostrea gigas. We selected fast- and slow-growing animals, and we exposed them to high and low...
Tipo: Text Palavras-chave: Energetics; Health; Herpesvirus; Nutrition; Physiological trade-off.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00513/62431/66716.pdf
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Establishing causal link between an infectious agent and mortalities in marine molluscan aquaculture on the example of Bonamia ostreae and Herpèsvirosis in oysters : proposal of a causal grid analysis ArchiMer
Thebault, Anne; Cochennec, Nathalie; Arzul, Isabelle; Renault, Tristan.
In marine molluscan aqauculture, infectious agents are regularly cited in literature associated with mass mortalities as emergin diseases, but the causal link is often based on very few argumeents. 24 ways to demonstrate causal links from epidemiolgical, ecological and marine pathology literature feasible for marine molluscs are given. Those criteria were applied to the ewample of Bonamia ostreae for Ostrea edulis and Herpesvirus for spats and larvae of Crassostrea gigas. Three principles could be applied in marine field for choosing criteria wich should be taken in priority : the demonstration must be given at three levels of organization, organ individual, population, the demonstration must keeps maximum epidemiological criterias, and then mixes...
Tipo: Text Palavras-chave: Herpesvirus; Bonamia ostrea; Crassostrea gigas; Ostrea edulis; Marine pathology; Ecology; Epidemiology; Infectious agents; Molluscan aquaculture.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-3341.pdf
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Detection of ostreid herpesvirus-1 (OsHV-1) by PCR using a rapid and simple method of DNA extraction from oyster larvae ArchiMer
Batista, Frederico; Taris, Nicolas; Boudry, Pierre; Renault, Tristan.
A DNA extraction procedure was developed for the detection of ostreid herpesvirus-1 (OsHV-1) using the polymerase chain reaction (PCR) in oyster larvae. The DNA extraction procedure developed was tested on 8 larval samples. Abnormal nuclei with characteristic features associated with OsHV-1 infections were only observed in samples in which the viral DNA was detected by PCR. A previously described competitive PCR method was applied to detect inhibition during PCR reactions. The results show that the method can be used on small amounts of oyster larvae (3 mg) for the detection of OsHV-1 DNA by PCR.
Tipo: Text Palavras-chave: DNA extraction; Detection; Larvae; Oyster; OsHV 1; Herpesvirus.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-2916.pdf
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Infection à virus de type herpès chez les larves de coquilles Saint-Jacques ArchiMer
Arzul, Isabelle; Nicolas, Jean-louis; Renault, Tristan.
King scallop (Pecten maximus) larvae suffered massive mortality during the summer of 2000 at a hatchery located in Brittany. Samples were taken for various types of analyses.
Tipo: Text Palavras-chave: Pathologie; Pecten maximus; Coquilles St Jacques; Herpesvirus; Herpesviridae.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/acte-3267.pdf
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French scallops: A new host for ostreid herpesvirus-1 ArchiMer
Arzul, Isabelle; Nicolas, Jean-louis; Davison, Andrew J.; Renault, Tristan.
Sporadic high mortalities were reported among larval French scallops (Pecten maximus). Electron microscopy of moribund larvae revealed particles with the characteristics of a herpesvirus in association with cellular lesions. PCR and DNA sequencing showed that the virus is a variant of ostreid herpesvirus-1 that has already been described in clams and oysters. This is the first description of a herpesvirus infection of a scallop species. The virus was transmitted successfully from an extract of infected scallop larvae to uninfected scallop or oyster (Crassostrea, gigas) larvae, demonstrating that it is able to infect both species. Detection of viral DNA in asymptomatic adult scallops by in situ hybridisation indicates that the herpesvirus may have been...
Tipo: Text Palavras-chave: Bivalve; Host range; Transmission; Polymorphism; OsHV 1; Pecten maximus; Scallop; Herpesvirus.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-739.pdf
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Emergence de maladies chez les organismes d'intérêt aquacole : quelques scénarios illustrés d'exemples ArchiMer
Saulnier, Denis; Reynaud, Yann; Arzul, Isabelle; Miossec, Laurence; Le Roux, Frédérique; Goarant, Cyrille.
According to the world organisation for animal health (OIE) an emerging disease is defined as a recently admitted serious illness, whose aetiology can, or not, have already been established, and which is likely to be propagated within a population or between populations, for example at the time of international exchanges of aquatic animals and/or products of aquatic animals. Even though the emerging diseases that affect human health have been much studied, those which affect marine organisms and species of aquaculture interest in particular are poorly documented. By restricting emergence to only infectious diseases, we aim at presenting in a non-exhaustive way some scenarios of the emergence of the diseases of aquacultured species by illustrating them with...
Tipo: Text Palavras-chave: Cyprinus carpio; Ostrea edulis; Bonamia ostreae; Peneides; Vibrio nigripulchritudo; Herpesvirus; Agent pathogène.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2980.pdf
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Recherche d'AND d'herpèsvirus infectant les bivalves dans des échantillons d'eau de claires ostréicoles ArchiMer
Solliec, Gaëlle; Vigneron, Valérie; Montanie, Hélène; Renault, Tristan.
Viral particles, associated with high mortality rates, have been reported among larvae and juveniles in different marine bivalve specie and characterized as member of the Hespesviridae family. Most studies focused on the detection of such viral infections among economically important species. However, the persistence of bivalve herpes viruses in marine environment is poorly documented. This topic appears as an important research domain in order to clarify the natural infection cycle of the herpesvirus of marine bivalves.
Tipo: Text Palavras-chave: Herpesviridae; Oyster; PCR; DNA; Herpesvirus; Huître; PCR; ADN.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-2856.pdf
Registros recuperados: 31
Primeira ... 12 ... Última
 

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