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Simultaneous determination of abamectin homologs H 2 B 1a and H 2 B 1b in gel formulation by high performance liquid chromatography BJPS
Alexandre,Grazielle Prado; Aurora-Prado,María Segunda; Español Mariño,Laura Victoria; Singh,Anil Kumar; Leite,Helen Dutra; Kedor-Hackmann,Erika Rosa Maria; Santoro,Maria Inês Rocha Miritello.
ABSTRACT Abamectin is a drug with antiparasitic properties used in several pharmaceutical formulations. The objective of this research was to develop and validate a high performance liquid chromatographic (HPLC) method for quantification of the two abamectin homologs (H2B1a and H2B1b) in gel formulation. This HPLC method was validated using a LichroCart(r) 100 RP-18 (125 x 4 mm, 5 µm) column. The mobile phase contained of acetonitrile and water (95:5 v/v) with 1% acetic acid. The flow rate was 1.0 mL min-1 and UV detection was performed at 245 nm. Mobile phase solutions were prepared containing a nominal concentration 185.2 µg mL-1 H2B1a and 9.6 µg mL-1 H2B1b. The method displayed good linearity in the concentration range of 148.1 - 222.3 µg mL-1 and 7.7 -...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abamectin homologs/validation method/gel formulation; Abamectin homologs; High Perfomance Liquid Chromatography (HPLC)..
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300509
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