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How does yeast respond to pressure? BJMBR
Fernandes,P.M.B..
The brewing and baking yeast Saccharomyces cerevisiae has been used as a model for stress response studies of eukaryotic cells. In this review we focus on the effect of high hydrostatic pressure (HHP) on S. cerevisiae. HHP exerts a broad effect on yeast cells characteristic of common stresses, mainly associated with protein alteration and lipid bilayer phase transition. Like most stresses, pressure induces cell cycle arrest. Below 50 MPa (500 atm) yeast cell morphology is unaffected whereas above 220 MPa wild-type cells are killed. S. cerevisiae cells can acquire barotolerance if they are pretreated with a sublethal stress due to temperature, ethanol, hydrogen peroxide, or pressure. Nevertheless, pressure only leads to protection against severe stress if,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharomyces cerevisiae; Stress adaptation; Gene expression; High hydrostatic pressure.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800012
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Prediction of acid lactic-bacteria growth in turkey ham processed by high hydrostatic pressure BJM
Mathias,S.P.; Rosenthal,A.; Gaspar,A.; Aragão,G.M.F.; Slongo-Marcusi,A..
High hydrostatic pressure (HHP) has been investigated and industrially applied to extend shelf life of meat-based products. Traditional ham packaged under microaerophilic conditions may sometimes present high lactic acid bacteria population during refrigerated storage, which limits shelf life due to development of unpleasant odor and greenish and sticky appearance. This study aimed at evaluating the shelf life of turkey ham pressurized at 400 MPa for 15 min and stored at 4, 8 and 12 ºC, in comparison to the non pressurized product. The lactic acid bacteria population up to 10(7) CFU/g of product was set as the criteria to determine the limiting shelf life According to such parameter the pressurized sample achieved a commercial viability within 75 days when...
Tipo: Info:eu-repo/semantics/article Palavras-chave: High hydrostatic pressure; Predictive models; Lactic acid bacteria; Turkey ham.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100003
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Effect of high hydrostatic pressure on antioxidant content of 'Ataulfo' mango during postharvest maturation Ciênc. Tecnol. Aliment.
Ortega,Viviana Guadalupe; Ramírez,José Alberto; Velázquez,Gonzalo; Tovar,Beatriz; Mata,Miguel; Montalvo,Efigenia.
The objective of this study was to evaluate the effect of pressurization on the concentration of some antioxidant compounds and the antiradical efficiency during the ripening process of 'Ataulfo' mango. The fruits at physiological maturity stage were pressurized at 15, 30, or 60 MPa for 10 or 20 min. Control fruits were not pressurized. The fruits were stored at 25 °C and changes in the concentration of ascorbic acid, total phenols, total flavonoids, total carotenoids, and antiradical efficiency were evaluated. It was demonstrated that in 'Ataulfo' mango high hydrostatic pressure treatments at 60 and 30 MPa for 20 minutes induced the synthesis of ascorbic acid during storage maybe as a consequence of physiological changes and possible structural...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mangifera indica L; High hydrostatic pressure; Antioxidants.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000300027
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Pyrococcus CH1, an obligate piezophilic hyperthermophile: extending the upper pressure-temperature limits for life ArchiMer
Zeng, Xiang; Birrien, Jean-louis; Fouquet, Yves; Cherkashov, Georgy; Jebbar, Mohamed; Querellou, Joel; Oger, Philippe; Cambon-bonavita, Marie-anne; Xiao, Xiang; Prieur, Daniel.
A novel hydrothermal site was discovered in March 2007, on the mid-Atlantic ridge during the cruise 'Serpentine'. At a depth of 4100 m, the site 'Ashadze' is the deepest vent field known so far. Smoker samples were collected with the ROV 'Victor 6000' and processed in the laboratory for the enrichment of anaerobic heterotrophic microorganisms under high-temperature and high-hydrostatic pressure conditions. Strain CH1 was successfully isolated and assigned to the genus Pyrococcus, within the Euryarchaeota lineage within the Archaea domain. This organism grows within a temperature range of 80 to 108 degrees C and a pressure range of 20 to 120 MPa, with optima for 98 degrees C and 52 MPa respectively. Pyrococcus CH1 represents the first obligate piezophilic...
Tipo: Text Palavras-chave: Thermococcales; Extremophile; Deep sea hydrothermal vent; High hydrostatic pressure; Archaea.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6812.pdf
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Restoration of the di-myo-inositol-phosphate pathway in the piezo-hyperthermophilic archaeon Thermococcus barophilus ArchiMer
Cario, Anais; Mizgier, Alex; Thiel, Axel; Jebbar, Mohamed; Oger, Phil M..
Most Thermococcales accumulate di-myo-inositol-phosphate (DIP) as an organic solute as a response to heat stress. We have studied the accumulation of this osmolyte in the high-hydrostatic pressure adapted hyperthermophile Thermococcus barophilus. We found no accumulation of DIP under any of the stress conditions tested, although this archaeon harbors the 3 DIP synthesis genes. Lack of synthesis is due to the lack of expression of TERMP_01135 coding for the second step of DIP synthesis. In contrast to other species, the T. barophilus synthesis operon is interrupted by a four gene locus, in reverse orientation. Restoring an operon like structure at the DIP locus restored DIP synthesis, but did not have an impact on growth characteristics, suggesting that...
Tipo: Text Palavras-chave: High hydrostatic pressure; Heat stress; Di-myo-inositol-phosphate; Thermococcus barophilus.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00270/38113/37216.pdf
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The many ways of coping with pressure ArchiMer
Oger, Philippe M.; Jebbar, Mohamed.
The current paper reviews strategies employed by microorganisms from the deep biosphere, especially piezophiles (from the greek piezo = to press and philo = love), to cope with high hydrostatic pressure (HHP) prevailing in these biotopes. The aim of this review is not to constitute an exhaustive report of our current knowledge on the physiology of piezophiles, as recent reviews have covered part of this subject in detail (Abe, 2007; Lauro and Bartlett, 2008; Michiels et al., 2008; Simonato et al., 2006). Rather, we illustrate here, via a few chosen examples, where we stand in our understanding of the mechanisms employed by microorganisms from the depths of our planet to cope with HHP. (C) 2010 Institut Pasteur. Published by Elsevier Masson SAS. All rights...
Tipo: Text Palavras-chave: Adaptation and evolution; Archaea; Deep biosphere; High hydrostatic pressure; Piezophile; Stress response.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00027/13797/11043.pdf
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