Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados BJPS (4) Autor Dhanani,Tushar (1) Donepudi,Sharmila (1) Farago,Paulo Vitor (1) Ferrari,Priscileila Colerato (1) Khalil,Najeh Maissar (1) Klein,Traudi (1) Kumar,Satyanshu (1) Langoski,Gisele (1) Lopes,Clarissa Elize (1) Mainardes,Rubiana Mara (1) Mattos,Ana Cristina de (1) Suneetha,Achanta (1) Mais... Palavra-chave High performance liquid chromatography/method validation (4) High performance liquid chromatography/quantitative analysis (2) Axitinib/ kidney cancer treatment (1) Axitinib/determination (1) Crizotinibe (1) Eclipta alba/analysis (1) Eclipta alba/extracts (1) Fluorouracil/determination (1) Halcinonide/encapsulation efficiency (1) Halcinonide/quantification (1) High performance liquid chromatography/photo diode array (1) Nanoparticles (1) Polymeric lipid-core nanoparticles (1) Rabbit plasma (1) Solid lipid nanoparticles (1) Topical administration (1) Toxicity (1) Tyrosine kinase inhibitor (1) Wedelolactone/quantification (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Ano 2017 (2) 2013 (2) País Brazil (4) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Development and validation of a rapid high performance liquid chromatography - photodiode array detection method for estimation of a bioactive compound wedelolactone in extracts of Eclipta alba BJPS Kumar,Satyanshu; Dhanani,Tushar. Following optimization of extraction, separation and analytical conditions, a rapid, sensitive and simple reverse-phase high performance liquid chromatography-photo diode array (HPLC-PDA) method has been developed for the identification and quantification of wedelolactone in different extracts of Eclipta alba. The separation of wedelolactone was achieved on a C18 column using the solvent system consisting of a mixture of methanol: water: acetic acid (95: 5: 0.04) as a mobile phase in isocratic elution mode followed by photo diode array detection at 352 nm. The developed method was validated as per the guidelines of the International Conference on Harmonization (ICH). Calibration curve presented good linear regression (r²>0.998) within the test range and... Tipo: Info:eu-repo/semantics/article Palavras-chave: High performance liquid chromatography/quantitative analysis; High performance liquid chromatography/photo diode array; High performance liquid chromatography/method validation; Eclipta alba/extracts; Eclipta alba/analysis; Wedelolactone/quantification. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100007 A simple HPLC method for the determination of halcinonide in lipid nanoparticles: development, validation, encapsulation efficiency, and in vitro drug permeation BJPS Lopes,Clarissa Elize; Langoski,Gisele; Klein,Traudi; Ferrari,Priscileila Colerato; Farago,Paulo Vitor. ABSTRACT Halcinonide is a high-potency topical glucocorticoid used for skin inflammation treatments that presents toxic systemic effects. A simple and quick analytical method to quantify the amount of halcinonide encapsulated into lipid nanoparticles, such as polymeric lipid-core nanoparticles and solid lipid nanoparticles, was developed and validated regarding the drug's encapsulation efficiency and in vitro permeation. The development and validation of the analytical method were carried out using the high performance liquid chromatography with the UV detection at 239 nm. The validation parameters were specificity, linearity, precision and accuracy, limits of detection and quantitation, and robustness. The method presented an isocratic flow rate of 1.0... Tipo: Info:eu-repo/semantics/article Palavras-chave: Halcinonide/encapsulation efficiency; Halcinonide/quantification; Polymeric lipid-core nanoparticles; Solid lipid nanoparticles; Topical administration; Toxicity; High performance liquid chromatography/method validation. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200604 HPLC method development and validation for the estimation of Axitinib in rabbit plasma BJPS Suneetha,Achanta; Donepudi,Sharmila. ABSTRACT A rapid, sensitive, and accurate high performance liquid chromatography for the determination of Axitinib (AN) in rabbit plasma is developed using crizotinibe as an internal standard (IS). Axitinib is a tyrosine kinase inhibitor, used in the treatment of advanced kidney cancer, which works by slowing or stopping the growth of cancer cells. The chromatographic separation was performed on a Waters 2695, Kromosil (150 mm × 4.6 mm, 5 µm) column using a mobile phase containing buffer (pH 4.6) and acetonitrile in the ratio of 65:35 v/v with a flow rate of1 mL/min. The analyte and internal standard were extracted using liquid-liquid extraction with acetonitrile. The elution was detected by photo diode array detector at 320 nm.The total chromatographic... Tipo: Info:eu-repo/semantics/article Palavras-chave: High performance liquid chromatography/method validation; Axitinib/determination; Axitinib/ kidney cancer treatment; Crizotinibe; Rabbit plasma; Tyrosine kinase inhibitor. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300607 Development and validation of an HPLC method for the determination of fluorouracil in polymeric nanoparticles BJPS Mattos,Ana Cristina de; Khalil,Najeh Maissar; Mainardes,Rubiana Mara. The objective of this work was to develop and validate a rapid high performance liquid chromatography (HPLC) method for the quantitative analysis of fluorouracil (5-FU) in polymeric nanoparticles. Chromatographic analyses were performed on an RP C18 column with a mobile phase consisting of acetonitrile and water (10:90, v/v) at a flow rate of 1 mL/min. The 5-FU was detected and quantitated using a photodiode array detector at a wavelength of 265 nm. The method was shown to be specific and linear in the range of 0.1-10 µg/mL (r = 0.9997). The precision (intra- and inter-day) was demonstrated because the maximum relative standard deviation was 3.51%. The method is robust relative to changes in flow rate, column and temperature. The limits of detection and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Fluorouracil/determination; Nanoparticles; High performance liquid chromatography/quantitative analysis; High performance liquid chromatography/method validation. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100013 Registros recuperados: 4 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco