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| Silva,Pedro Henrique Reis da; Enéas,Paula Cristina Rezende; Fernandes,Christian; Pianetti,Gerson Antônio. |
| ABSTRACT Prednisone is an anti-inflammatory steroid drug widely used in clinical practice. However, no high-performance liquid chromatographic (HPLC) method has been described in the literature for the determination of prednisone in capsules until now. Thus, an HPLC method was developed using a C18 (250x4.0, 5 µm) column, with methanol:water (70:30) as mobile phase at a flow rate of 1 mL/min and detection at 240 nm. The developed method was validated following current Brazilian legislation. Additionally, linearity was assessed by evaluating the assumptions of normality, homoscedasticity, and independency of residuals, and the fit to the linear model. The method showed linearity (r2>0.99) over the range of 14.0-26.0 µg/mL, selectivity, precision... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Prednisone; Capsules; High performance liquid chromatography/validation; Linearity assessment.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200624 |
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| Lima,Isabela Angeli de; Khalil,Najeh Maissar; Mainardes,Rubiana Mara. |
| ABSTRACT The development and validation of a simple and efficient method for the quantification of ferulic acid in poly (D,L-lactide-co-glycolide) (PLGA) nanoparticles coated with chitosan (CS) by reverse phase high performance liquid chromatography coupled to photodiode array detection was described. For the chromatographic analysis, a reverse phase C-18 column was used, mobile phase consisting of acetonitrile and 0.5% acetic acid (37:63, v/v), isocratically eluted at a flow rate of 1 mL/min. Drug determination was performed at 320 nm. The method was validated in terms of the selectivity, linearity, precision, accuracy, robustness, limits of detection and quantification. The method was linear in the range of 10 to 100 μg/mL (r=0.999) and presented limit... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nanoparticles; Ferulic acid/encapsulation efficiency; Ferulic acid/quantification; Stability; High performance liquid chromatography/validation; PLGA-poly(lactide-co-glycolide)/nanoparticles; Chitosan.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200627 |
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