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Characterization of pyrene utilizing Bacillus spp. from crude oil contaminated soil BJM
Khanna,Purnima; Goyal,Dinesh; Khanna,Sunil.
Pyrene, a high molecular weight polycyclic aromatic hydrocarbon (PAH), is a priority pollutant present in soil contaminated with crude oil, coal-tar and complex PAHs. Bacterial consortium CON-3 developed from crude oil contaminated soil of Patiala, Punjab (India) cometabolized 50 µg ml-1 pyrene in the presence of glucose (0.5 %; w/v) at 30 °C, as determined by reverse-phase high performance liquid chromatography (HPLC). Bacillus sp. PK-12, Bacillus sp. PK-13 and Bacillus sp. PK-14 from CON-3, identified by 16S rRNA gene sequence analysis, were able to cometabolize 64 %, 55 % and 53 % of pyrene in 35 days, respectively. With the increase in glucose concentration to 1.0 % (w/v) in growth medium isolates PK-12, PK-13 and PK-14 showed 19 - 46 % uptake of 50 µg...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pyrene; Bacillus; Crude oil contaminated soil; High performance liquid chromatography (HPLC).
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200024
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Degradation of Benzo [a] Pyrene by a novel strain Bacillus subtilis BMT4i (MTCC 9447) BJM
Lily,Madhuri Kaushish; Bahuguna,Ashutosh; Dangwal,Koushalya; Garg,Veena.
Benzo [a] Pyrene (BaP) is a highly recalcitrant, polycyclic aromatic hydrocarbon (PAH) with high genotoxicity and carcinogenicity. It is formed and released into the environment due to incomplete combustion of fossil fuel and various anthropogenic activities including cigarette smoke and automobile exhausts. The aim of present study is to isolate bacteria which can degrade BaP as a sole source of carbon and energy. We have isolated a novel strain BMT4i (MTCC 9447) of Bacillus subtilis from automobile contaminated soil using BaP (50 μg /ml) as the sole source of carbon and energy in basal salt mineral (BSM) medium. The growth kinetics of BMT4i was studied using CFU method which revealed that BMT4i is able to survive in BaP-BSM medium up to 40 days attaining...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Benzo [a] Pyrene (BaP); Polycyclic aromatic hydrocarbon (PAH); Bacillus subtilis BMT4i; Colony forming units/ml (CFU/ml); High performance liquid chromatography (HPLC).
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400020
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Quantification of catechins and caffeine from green tea (Camellia sinensis) infusions, extract, and ready-to-drink beverages Ciênc. Tecnol. Aliment.
Oliveira,Rafaela Macedo Mendes De.
This study aimed to quantify the levels of catechins and caffeine in various forms of presentation of green tea: infusion tea bags, extract, and ready-to-drink beverage and, based on their content, identify the most suitable for consumption. High Performance Liquid Chromatography (HPLC) analytical method was used for the quantification of catechins and caffeine. The tea bags had the highest concentration of total catechins with 5 to 9.5% followed by the extract with 3.64 to 4.88%, and ready-to-drink green tea beverage showed low levels of catechins, from 0.14 to 0.26%. As for caffeine content, green tea extract had higher concentration (1.96 to 3.54%) compared to the tea bags (1.39 to 1.57%). Tea bags were found the most suitable for consumption because it...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Green tea; Catechins; Caffeine; High performance liquid chromatography (HPLC).
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000100024
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Vitamin C content in Habanero pepper accessions (Capsicum chinense) Horticultura Brasileira
Teodoro,Ana Flávia P; Alves,Rosa de BN; Ribeiro,Leandro B; Reis,Karina; Reifschneider,Francisco José B; Fonseca,Maria Esther de N; Silva,Joseane P da; Agostini-Costa,Tânia da S.
Fruits of Capsicum species (peppers) accumulate high amounts of ascorbic acid or vitamin C. C. chinense occurs in the Midwest and Northeast regions and the Amazon Basin (where its greatest genetic diversity is found). The objective of the present work was to quantify the vitamin C content in peppers of 22 accessions of C. chinense 'Habanero' from the Breeding Program of Embrapa Vegetable Crops. Vitamin C was extracted from mature fruits with TCEP-HCl (tris 2-carboxyethyl-phosphine hydrocloride) and its content determined by high performance liquid chromatography (HPLC). Vitamin C content ranged from 54.1 to 129.8 mg/100 g. Accessions were divided into four heterogeneous groups of diversity. Vitamin C content of the first group varied between 116.2 and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pepper; Ascorbic acid; Genetic resource; High performance liquid chromatography (HPLC).
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000100009
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