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Semi-scale production of PHAs from waste frying oil by Pseudomonas fluorescens S48 BJM
Gamal,Rawia F.; Abdelhady,Hemmat M.; Khodair,Taha A.; El-Tayeb,Tarek S.; Hassan,Enas A.; Aboutaleb,Khadiga A..
The present study aimed at developing a strategy to improve the volumetric production of PHAs by Pseudomonas fluorescens S48 using waste frying oil (WFO) as the sole carbon source. For this purpose, several cultivations were set up to steadily improve nutrients supply to attain high cell density and high biopolymer productivity. The production of PHAs was examined in a 14 L bioreactor as one-stage batch, two-stage batch, and high-cell-density fed-batch cultures. The highest value of polymer content in one-stage bioreactor was obtained after 60 h (33.7%). Whereas, the two-stage batch culture increased the polymer content to 50.1% after 54 h. High-cell-density (0.64 g/L) at continuous feeding rate 0.55 mL/l/h of WFO recorded the highest polymer content after...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas fluorescens S48; Biopolymer; PHAs; Batch; Two-stage batch; High-celldensity fed batch; Bioreactor; Recovery.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200034
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