| An experiment using signalgrass hay treated with increasing urea levels (0, 20, 40, and 80 g/kg of dry matter), and submitted to in vitro digestion times (0, 6, 12, 24 or 72 hours) was conducted to evaluate the effects of ammoniation rates and digestion times on leaf blade tissue degradation. A completely randomized design with a 4 × 5 factorial treatment arrangement, with three replicates was used. Data were submitted to multivariate analysis. Urea-treated hay presented smaller proportion of remaining tissues measured in leaf blades cross-sections after in vitro digestion. Tissues more affected by urea treatment degradation were parenchyma bundle sheath and sclerenchyma. The hay treated with 80 g urea/kg DM and in vitro digested for 24 or 72 hours showed... |
