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| KASSAR,TELISSA C.; MAGALHÃES,TEREZA; S. JÚNIOR,JOSÉ V.J.; CARVALHO,AMANDA G.O.; SILVA,ANDRÉA N.M.R. DA; QUEIROZ,SABRINA R.A.; BERTANI,GIOVANI R.; GIL,LAURA H.V.G.. |
| ABSTRACT Yellow fever is an arthropod-borne viral disease that still poses high public health concerns, despite the availability of an effective vaccine. The development of recombinant viruses is of utmost importance for several types of studies, such as those aimed to dissect virus-host interactions and to search for novel antiviral strategies. Moreover, recombinant viruses expressing reporter genes may greatly facilitate these studies. Here, we report the construction of a recombinant yellow fever virus (YFV) expressing Gaussia luciferase (GLuc) (YFV-GLuc). We show, through RT-PCR, sequencing and measurement of GLuc activity, that stability of the heterologous gene was maintained after six passages. Furthermore, a direct association between GLuc... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gaussia luciferase; Homologous recombination in yeast; Reporter gene; Yellow fever virus. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502119 |
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