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	Provedor de dados BJMBR (1) Genet. Mol. Biol. (1) Autor Iwatani,Yoshinori (1) Krepischi,A.C.V. (1) Matsuzuka,Fumio (1) Miyauchi,Akira (1) Pereira,L.V. (1) Stabellini,R. (1) Takano,Toru (1) Vasques,L.R. (1) Watanabe,Mikio (1) Yamada,Hiroya (1) de Araújo,E.S.S. (1) Mais... Palavra-chave Human cell line (2) 5-aza-2′-deoxycytidine (1) DNA methylation (1) Imprinted genes (1) Stem cell (1) TTF-1 (1) Thyroid (1) Transcription (1) XIST (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2014 (1) 2011 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Stability of XIST repression in relation to genomic imprinting following global genome demethylation in a human cell line BJMBR de Araújo,E.S.S.; Vasques,L.R.; Stabellini,R.; Krepischi,A.C.V.; Pereira,L.V.. DNA methylation is essential in X chromosome inactivation and genomic imprinting, maintaining repression of XIST in the active X chromosome and monoallelic repression of imprinted genes. Disruption of the DNA methyltransferase genes DNMT1 and DNMT3B in the HCT116 cell line (DKO cells) leads to global DNA hypomethylation and biallelic expression of the imprinted gene IGF2 but does not lead to reactivation of XIST expression, suggesting thatXIST repression is due to a more stable epigenetic mark than imprinting. To test this hypothesis, we induced acute hypomethylation in HCT116 cells by 5-aza-2′-deoxycytidine (5-aza-CdR) treatment (HCT116-5-aza-CdR) and compared that to DKO cells, evaluating DNA methylation by microarray and monitoring the expression of... Tipo: Info:eu-repo/semantics/article Palavras-chave: XIST; Imprinted genes; DNA methylation; 5-aza-2′-deoxycytidine; Human cell line. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201029 Transcriptional activity of the 5'-flanking region of the thyroid transcription factor-1 gene in human thyroid cell lines Genet. Mol. Biol. Yamada,Hiroya; Takano,Toru; Matsuzuka,Fumio; Watanabe,Mikio; Miyauchi,Akira; Iwatani,Yoshinori. Thyroid transcription factor-1 (TTF-1, NKX2-1) is a homeodomain-containing transcriptional factor that binds to and activates the promoters of thyroid and lung-specific genes, such as thyroglobulin, thyroid peroxidase, and thyroid stimulating hormone receptor. TTF-1 is known to play a key role in the development of the thyroid. However, the precise mechanism of TTF-1 gene transcription in human thyroid cells has not been studied. The expression of transcriptional activity in various lengths of the 5'-flanking region of the human TTF -1 gene was studied in TTF-1 positive and negative human thyroid cell lines. Increased transcriptional activity was observed in thyroid cell lines containing plasmids that coded for a sequence proximal to the transcription... Tipo: Info:eu-repo/semantics/article Palavras-chave: Thyroid; TTF-1; Transcription; Human cell line; Stem cell. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100002 Registros recuperados: 2 Primeira ... 1 ... Última

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