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Oviduct-specific expression of tissue plasminogen activator in laying hens Genet. Mol. Biol.
Kaleri,Hubdar Ali; Xiang,Liu; Aniwashi,Jueken; Xu,Shiyong.
Egg-laying hens are important candidate bioreactors for pharmaceutical protein production because of the amenability of their eggs for protein expression. In this study, we constructed an oviduct-specific vector containing tissue plasminogen activator (tPA) protein and green fluorescent protein (pL-2.8OVtPAGFP) and assessed its expression in vitro and in vivo. Oviduct epithelial and 3T3 cells were cultured and transfected with pL-2.8OVtPAGFP and pEGP-N1 (control vector), respectively. The pL-2.8OVtPAGFP vector was administered to laying hens via a wing vein and their eggs and tissues were examined for tPA expression. The oviduct-specific vector pL-2.8OVtPAGFP was expressed only in oviduct epithelial cells whereas pEGP-N1 was detected in oviduct epithelial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Green fluorescent protein; Human tissue plasminogen activator; Laying hens; Oviduct-specific expression.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200011
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