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In vitro Antimicrobial Assay of Actinomycetes in Rice AgainstXanthomonas oryzae pv. oryzicola and as Potential Plant Growth Promoter BABT
Hata,Erneeza Mohd; Sijam,Kamaruzaman; Ahmad,Zainal Abidin Mior; Yusof,Mohd Termizi; Azman,Noor Aisyah.
ABSTRACT The aim of this work was to invitro assay the antimicrobial activity of actinomycetes in rice against Xanthomonas oryzae pv. oryzicola and as potential plant growth promoter. A total of 92 actinomycete strains were isolated from different rice plant components and field locations. Of these, only 21.74% showed antagonistic activity against the Xoc pathogen. Molecular identification via 16s rRNA amplification revealed that 60% of the active antagonistic strains belonged to the genus Streptomyces. Isolates that demonstrated the highest antagonistic activity were also able to produce hydrolytic enzymes and plant growth-promoting hormones. Combination of preliminary screening based on in vitro antagonistic, hydrolytic enzyme and plant growth hormone...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antagonistic activity; Hydrolytic enzyme; Plant growth-promoting agent; Streptomyces.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600821
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Endophytic fungi from medicinal plant Bauhinia forficata: Diversity and biotechnological potential BJM
Bezerra,Jadson D.P.; Nascimento,Carlos C.F.; Barbosa,Renan do N.; Silva,Dianny C.V. da; Svedese,Virgínia M.; Silva-Nogueira,Eliane B.; Gomes,Bruno S.; Paiva,Laura M.; Souza-Motta,Cristina M..
Bauhinia forficata is native to South America and used with relative success in the folk medicine in Brazil. The diversity, antibacterial activity, and extracellular hydrolytic enzymes of endophytic fungi associated with this plant were studied. Plant samples, which included leaves, sepals, stems, and seeds, were used. Ninety-five endophytic fungal were isolated (18 from leaves, 22 from sepals, 46 from stems, and nine from seeds), comprising 28 species. The most frequently isolated species were Acremonium curvulum (9.5%), Aspergillus ochraceus (7.37%), Gibberella fujikuroi (10.53%), Myrothecium verrucaria (10.53%) and Trichoderma piluliferum(7.37%). Diversity and species richness were higher in stem tissues, and Sorensen’s index of similarity between the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibacterial agents; Fungal endophytes; Hydrolytic enzyme; Symbiosis.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100049
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Stable expression and characterization of a fungal pectinase and bacterial peroxidase genes in tobacco chloroplast Electron. J. Biotechnol.
Espinoza-Sánchez,Edward Alexander; Álvarez-Hernández,Marianela Hazel; Torres-Castillo,Jorge Ariel; Rascón-Cruz,Quintín; Gutiérrez-Díez,Adriana; Zavala-García,Francisco; Sinagawa-García,Sugey Ramona.
Background The high capacity of chloroplast genome response to integrate and express transgenes at high levels makes this technology a good option to produce proteins of interest. This report presents the stable expression of Pectin lyase (PelA gene) and the first stable expression of manganese peroxidase (MnP-2 gene) from the chloroplast genome. Results pES4 and pES5 vectors were derived from pPV111A plasmid and contain the PelA and MnP-2 synthetic genes, respectively. Both genes are flanked by a synthetic rrn16S promoter and the 3'UTR from rbcL gene. Efficient gene integration into both inverted repeats of the intergenic region between rrn16S and 3'rps'12 was confirmed by Southern blot. Stable processing and expression of the RNA were confirmed by...
Tipo: Journal article Palavras-chave: Cell wall; Chloroplast genetic transformation; Hydrolytic enzyme; Nicotiana tabacum.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300004
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