|
|
|
|
|
| Xiaoyan,Liu; Xiangdong,Zhou. |
| This study aimed at identifying the molecular mechanisms and effects of hypertonicity on mucin5AC (MUC5AC) expression in airway epithelial cells for which immortalized human bronchial epithelial (16HBE) cells were cultured in 600 mOsm/L hypertonic medium for different times in vitro. Proteins of MUC5AC and β-catenin, Cyclin D1, NF-κB p65 were detected by enzyme linked immunosorbent assay (ELISA), reverse transcription (RT)-PCR and western blotting assays. After transfection of β-catenin siRNA in 16HBE cells, the levels of β-catenin, Cyclin D1, NF-κB p65 and MUC5AC protein were detected. Results showed that the levels of mRNAs and proteins of target genes increased significantly after the exposure to hypertonic conditions and the expression content... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MUC5AC; Hypertonicity; Wnt/β-catenin; NF-κB p65. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400006 |
| |
|
|
| McKinley,Michael; Trevaks,David; Weissenborn,Frank; McAllen,Robin. |
| ABSTRACT The ability to maintain core temperature as well as volume and composition of body fluids within narrow ranges is a major characteristic of mammals. Yet, the ability to maintain a stable core temperature often relies on physiological responses that perturb the stability of body fluids. A common thermoregulatory mechanism that affects body fluid homeostasis is evaporative cooling, by sweating and/or panting, to dissipate heat from the body when core temperature is elevated. However, these responses result in a reduction of total body water, thereby reducing blood volume and increasing the osmotic pressure of body fluids. While both panting and sweating are highly effective means of preventing core body temperature from increasing, unless the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brain cooling; Hypertonicity; Osmoreceptors; Panting; Sweating. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017000900783 |
| |
|
|
|
