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| Registros recuperados: 67 | |
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| Rosa, Daniel Dias; UNESP; Marque, Janaina Marianno de; UNESP; Fenille, Roseli Chela; Laboratorio de Apoio Vegetal - MAPA; Souza, Nilton Luiz de; UNESP; Kuramae, Eiko Eurya; Fungal Biodiversity Center. |
| Análise filogenética através de características morfológicas e culturais é considerada inconsistente e de difícil aceitação cientiífica, uma vez que não há como estabelecer a direção da evolução de caracteres morfológicos. Os mMarcadores moleculares são mais apropriados para análises filogenéticas. O seqüeênciamento das regiões ITS1 e ITS2 (Internal Transcribed Spacer) e o gene 5.8S rDNA forami utilizados para estimar a variação e a distância genética entre 10 isolados de Phytophthora capsici. A quantidade de variação genética entre os isolados de P. capsici de diferentes regiões do estadoEstado de São Paulo foi de 0,1% a 1,6% e de 0,1% a 1,1% nas regiões ITS1 e ITS2, respectivamente, e uma distância filogenética de 78,5% entre os isolados de P. capsici e... |
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Palavras-chave: 2.01.00.00-0 Biologia Geral DNA ribossomal; ITS; FFilogenia; Phytophthora sp 2.01.00.00-0 Biologia Geral. |
| Ano: 2003 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2033 |
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| Demirel,Rasime; Sariozlu,Nalan Yilmaz; İlhan,Semra. |
| In the present study, nine terverticillate Penicillium isolates (P. griseofulfum, P. puberulum, P. crustosum, P. aurantiogriseum, P. chrysogenum, P. primulinum, P. expansum, P. viridicatum, Eupenicillium egyptiacum) from 56 soil samples were characterized genetically by a PCR method. The DNAs of the strains were isolated using the glass beads and vortexing extraction method and then used for PCR amplification with the internal transcribed spacer 1 (ITS1) and ITS4 universal fungal specific primers. The ITS regions of fungal ribosomal DNA (rDNA) were sequenced through the CEQ 8000 Genetic Analysis System. ITS-5.8S sequences obtained were compared with those deposited in the GenBank Database. The results indicated that the identification of Penicillium... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Penicillium; Identification methods; PCR; ITS. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600013 |
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| Roslim,Dewi Indriyani. |
| ABSTRACT Pandan (Pandanus sp), Rotan (Calamus sp), and Rengas (Gluta sp) are the three most important plants growing at Kajuik Lake, Langgam, Riau Province, Indonesia; however, their species names have not been identified. This study aimed to identify their species names using nuclear internal transcribed spacer (ITS) and psbA-trnH intergenic spacer sequences. The method employed was DNA isolation from fresh leaves, PCR using primer pairs of ITS region for Pandanus sp and psbA-trnH intergenic spacer for Calamus sp and Gluta sp, electrophoresis, sequencing, and data analysis using BLASTn program and MEGA software version 6.0. Pandanus tectorius was the only one accession that was similar to Pandanus sp with the identity was 90%, however the query cover was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Calamus sp; Gluta sp; ITS; Pandanus sp; PsbA-trnH intergenic spacer. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100408 |
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| Andrade,Hortência Farias de; Araújo,Lívia Caroline Alexandre de; Santos,Bruno Souza dos; Paiva,Patrícia Maria Guedes; Napoleão,Thiago Henrique; Correia,Maria Tereza dos Santos; Oliveira,Maria Betânia Melo de; Lima,Gláucia Manoella de Souza; Ximenes,Rafael Matos; Silva,Túlio Diego da; Silva,Girliane Regina da; Silva,Márcia Vanusa da. |
| Abstract In this work, four isolates of endophytic fungi (Alternaria alternata, Colletotrichum gloesporioides, Glomerella cingulata and Nigrospora sphaerica), deposited in the culture collection ‘University Recife Mycologia’ (URM) at the Universidade Federal de Pernambuco, were characterized for the genes ITS 1 and 4 (region 5.8 S) and evaluated for taxol production. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Paclitaxel; Taxol route genes; ITS; Colletotrichum gloesporioides. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500059 |
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| Ragonezi,Carla; Caldeira,A. Teresa; Rosário Martins,M.; Salvador,Cátia; Santos-Silva,Celeste; Ganhão,Elsa; Klimaszewska,Krystyna; Zavattieri,Amely. |
| Stone pine (Pinus pinea L.), like other conifers, forms ectomycorrhizas (ECM), which have beneficial impact on plant growth in natural environments and forest ecosystems. An in vitro co-culture of stone pine microshoots with pure mycelia of isolated ECM sporocarps was used to overcome the root growth cessation not only in vitro but also to improve root development during acclimation phase. Pisolithus arhizus (Scop.) Rauschert and Lactarius deliciosus (L. ex Fr.) S.F. Gray fungi, were collected, pure cultured and used in in vitro co-culture with stone pine microshoots. Samples of P. arhizus and L. deliciosus for the in vitro co-cultures were collected from the pine stands southwest Portugal. The in situ characterization was based on their morphotypes. To... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pisolithus arhizus; Lactarius deliciosus; Pinus pinea; M13-PCR; ITS. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200047 |
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| Barguil,Beatriz Meireles; Oliveira,Sônia Maria Alves de; Coelho,Rildo Sartori Barbosa; Beserra Júnior,José Evando Aguiar. |
| A antracnose afeta a qualidade de inflorescências de plantas ornamentais tropicais, e a espécie fúngica Colletotrichum gloeosporioides tem sido relacionada a essa doença apenas por análises morfológicas. Por isso, o presente trabalho teve como objetivos identificar isolados de Colletotrichum coletados em plantas de antúrio (Anthurium andraeanum), bastão do imperador (Etlingera elatior) e helicônia (Heliconia spp.), por meio de caracteres morfológicos e reação em cadeia da polimerase (PCR), e avaliar a variabilidade genética por meio de oligonucleotídeos arbitrários (AP-PCR). Pelas características morfológicas de tamanho de conídio e de apressório, todos os isolados foram identificados como C. gloeosporioides. Um fragmento de 450pb específico para C.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anthurium andraeanum; AP-PCR; Etlingera elatior; Heliconia spp.; ITS. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000600002 |
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| Ferrada,Sandra; Hernández,Klaudia; Montoya,R.; Galleguillos,Ricardo. |
| Se estudió la variabilidad de 2 genes nucleares, el intrón 4/5 del gen de Calmodulina (CaM) por amplificación PCR y la región ITS (Internal Transcribed Spacers) del ADNr, por PCR y digestión en 139 ejemplares de E. ringens provenientes de las costas de Iquique (20°13'S; 70°09'W) y Talcahuano (36°41'S; 73°06'W). Para CaM se identificaron 8 genotipos en las muestras de Talcahuano y 7 en Iquique, presentando 1 a 3 fragmentos amplificados de 550 a 1.100 pb. En la digestión del fragmento ITS amplificado, sólo la endonucleasa TaqªI detectó 3 diferentes genotipos, 2 presentes en Talcahuano y 3 en Iquique. Se discuten las frecuencias observadas para cada gen y área de estudio. Los resultados llevan a confirmar la existencia de un solo stock, desde un punto de... |
| Tipo: Journal article |
Palavras-chave: Engraulis ringens; ITS; CaM; Variabilidad genética; Stock. |
| Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382002000200022 |
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| Ruiz,Eduardo; Toro,Oscar; Crawford,Daniel J; Stuessy,Tod F; Negritto,María A; Baeza,Carlos; Becerra,José. |
| Phylogenetic relationships among Chilean species of Drimys as well asD. granadensis from Central America andD. angustifolia from Brazil were reconstructed using internal transcribed spacers (ITS) of nuclear ribosomal DNA and a parsimony analysis. Our results at the family level were congruent with previous phylogenetic hypotheses. Molecular data indicate that the Juan Fernandez Island endemic D. confertifolia diverged early in the evolution of the genus, and it is not closely related to the continental species D. winteri, as might be expected on the basis of morphology and biogeography. The ITS phytogeny also provides general support for a recent distribution of species on the western side of the Andes Mountains; the only exception is D. andina, which may... |
| Tipo: Journal article |
Palavras-chave: Drimys; Evolution; ITS; Indels; Winteraceae. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432008000200008 |
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| Hershkovitz,Mark A. |
| Se determinaron las secuencias del espaciador interno (ITS) del ADN ribosomal y del espaciador intergénico de ycf3-trnS del ADN del cloroplasto para 183 muestras de taxas, chilenos y no chilenos, de Portulacaceae de América occidental, y sus grupos externos. Los datos permiten refinar previas inferencias sobre la circunscripción e interrelaciones de los géneros estudiados. En particular estos datos revelan que una circunscripción anteriormente publicada de Cistanthe Spach es polifilética y también revelan la existencia de un clado norteamericano compuesto por Claytonia L., Lewisia Pursh, Lewisiopsis R. Govaerts, and Montia L. De los datos de los géneros sudamericanos surgieron dos patrones: (1) en algunos casos la divergencia interespecífica es... |
| Tipo: Journal article |
Palavras-chave: Portulacaceae; Chile; ITS; Ycf3-trnS; Hibridización. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432006000100002 |
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| Muñoz,Manuel; Riegel,Ricardo; Seemann,Peter; Peñailillo,Patricio; Schiappacasse,Flavia; Núñez,José. |
| La taxonomía de los géneros chilenos de Amaryllidaceae es confusa, persistiendo problemas taxonómicos tanto a nivel de géneros como de especies. Actualmente existe controversia acerca de la correcta clasificación de especies hasta ahora asignadas a Rhodophiala C.Presl. A través del estudio de aspectos morfológicos, recientemente se ha sugerido la rehabilitación del género Rhodolirium Phil., donde Rhodolirium montanum Phil. es considerada como especie tipo. Esta especie ha sido denominada Rhodophiala rhodolirion (Baker) Traub en las clasificaciones tradicionales. Dado que los datos morfológicos han provisto información limitada y ambigua en la clasificación de las Amarilidáceas chilenas, este trabajo evalúa la pertinencia de formalizar la rehabilitación del... |
| Tipo: Journal article |
Palavras-chave: Rhodophiala; Rhodolirium montanum; Amaryllidaceae; ITS; Cariotipo. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432011000100005 |
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| Papini,Alessio; Banci,Francesca; Nardi,Enio. |
| An analysis of internal transcribed spacer (ITS) DNA sequences of the four species of Carum L. (Apiaceae) known in Italy revealed that this genus is polyphyletic. Maximum parsimony with bootstrap resampling, maximum likelihood and Bayesian inference analyses resulted in three distinct clades: Carum carvi L. clustered within tribe Careae Baill. (former Aegopodium clade); Hellenocarum multiflorum (= Carum multiflorum), Carum heldreichii and Carum appuanum clustered within the tribe Pyramidoptereae Boiss.; and H. multiflorum and C. heldreichii formed a well supported clade. Since the sister group of H. multiflorum and C. heldreichii was Bunium elegans the autonomy of Hellenocarum from Carum is confirmed by our study. We also found that C. appuanum clustered... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apiaceae; Carum; Hellenocarum; ITS; Phylogeny. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300029 |
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| Mäder,Geraldo; Castro,Luana; Bonatto,Sandro Luis; Freitas,Loreta Brandão de. |
| Abstract Non-indigenous plants exhibit different attributes that make them aggressive competitors with indigenous plants and serious threats to biodiversity.Senecio madagascariensis (fireweed, Asteraceae), a native from southern Africa, is a strong competitor in agricultural activities and has toxic alkaloids that may result in high cattle mortality. In Brazil, this weed was collected for the first time in 1995 and has since spread quickly throughout the Pampas region. To better understand the invasion of the fireweed in South America, we used a genetic characterization with internal transcribed spacer (ITS) and microsatellite markers. Based on the ITS data, the southern Brazil populations of S. madagascariensis shared genetic homology with samples taken... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microsatellites; ITS; Pampas; Weed; Spread. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100135 |
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| Mäder,Geraldo; Zamberlan,Priscilla M.; Fagundes,Nelson J.R.; Magnus,Tielli; Salzano,Francisco M.; Bonatto,Sandro L.; Freitas,Loreta B.. |
| The discovery and characterization of informative intraspecific genetic markers is fundamental for evolutionary and conservation genetics studies. Here, we used nuclear ribosomal ITS sequences to access intraspecific genetic diversity in 23 species of the genus Passiflora L. Some degree of variation was detected in 21 of these. The Passiflora and Decaloba (DC.) Rchb. subgenera showed significant differences in the sizes of the two ITS regions and in GC content, which can be related to reproductive characteristics of species in these subgenera. Furthermore, clear geographical patterns in the spatial distribution of sequence types were identified in six species. The results indicate that ITS may be a useful tool for the evaluation of intraspecific genetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic diversity; Intraspecific variability; ITS; Passiflora; Phylogeography. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100018 |
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| Benzoni, Francesca; Stefani, Fabrizio; Pichon, Michel; Galli, Paolo. |
| The morphometric and molecular boundaries between twelve Psammocora (Cnidaria, Scleractinia) nominal species were addressed. The type specimens of Psammocora haimiana Milne Edwards & Haime, 1851, P. togianensis Umbgrove, 1940, P. folium Umbgrove, 1939, P. digitata Milne Edwards & Haime, 1851, Maeandroseris australiae Rousseau, 1854, P. samoensis Hoffmeister, 1925, P. superficialis Gardiner, 1898, P. profundacella Gardiner, 1898, P. nierstraszi Van der Horst, 1921, P. verrilli Vaughan, 1907, and P. albopicta Benzoni, 2006, were analysed together with specimens from museum collections, including those depicted in widely cited taxonomic descriptions, and material collected for this study in different parts of the Indo-Pacific. Morphometric analyses of... |
| Tipo: Text |
Palavras-chave: 5; 8S; Calice morphometry; Enclosed petaloid septa; ITS; Phylogeny; Psammocora haimiana; Psammocora vaughani; Scleractinian coral. |
| Ano: 2010 |
URL: https://archimer.ifremer.fr/doc/00138/24941/23034.pdf |
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| Elgharsalli, Refka; Aloui-bejaoui, Nejla; Sala, Hedi; Chollet, Bruno; Joly, Jean-pierre; Robert, Maeva; Couraleau, Yann; Arzul, Isabelle. |
| Marteilia refringens is a protozoan parasite recognized as a significant pathogen of the European flat oyster Ostrea edulis. The life cycle of this species is still poorly known, although there is evidence of the need for intermediate host(s). In the present study, we have used molecular approaches to identify this parasite in samples of the dwarf oyster Ostrea stentina after reports of massive mortality along the Tunisian coasts. In 2009 we evaluated the status of O. stentina from Monastir and checked if there was an infection with M. refringens, using polymerase chain reaction assays. Of the 103 tested O. stentina, 85 were PCRpositive using a Marteilia genus-specific assay. Additional assays were subsequently carried out on some samples collected in 2010... |
| Tipo: Text |
Palavras-chave: Marteilia refringens; Molecular diagnosis; IGS; ITS; Transmission electron microscopy; Ostrea stentina. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00124/23538/21422.pdf |
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| Balzano, Sergio; Percopo, Isabella; Siano, Raffaele; Gourvil, Priscillia; Chanoine, Melanie; Marie, Dominique; Vaulot, Daniel; Sarno, Diana. |
| Seventy-five diatoms strains isolated from the Beaufort Sea (Canadian Arctic) in the summer of 2009 were characterized by light and electron microscopy (SEM and TEM) as well as 18S and 28S rRNA gene sequencing. These strains group into 20 genotypes and 17 morphotypes and are affiliated with the genera Arcocellulus, Attheya, Chaetoceros, Cylindrotheca, Eucampia, Nitzschia, Porosira, Pseudo-nitzschia, Shionodiscus, Thalassiosira, Synedropsis. Most of the species have a distribution confined to the northern/polar area. Chaetoceros neogracilis and Chaetoceros gelidus were the most represented taxa. Strains of C. neogracilis were morphologically similar and shared identical 18S rRNA gene sequences, but belonged to four distinct genetic clades based on 28S rRNA,... |
| Tipo: Text |
Palavras-chave: Biogeography; ITS; ITS2 secondary structure; LSU; Morphology; Phylogeny; Polar diatoms; SSU. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00356/46718/46584.pdf |
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| Registros recuperados: 67 | |
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