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| Azevedo,V.; Levitus,G.; Miyoshi,A.; Cândido,A.L.; Goes,A.M.; Oliveira,S.C.. |
| DNA-based immunization has initiated a new era of vaccine research. One of the main goals of gene vaccine development is the control of the levels of expression in vivo for efficient immunization. Modifying the vector to modulate expression or immunogenicity is of critical importance for the improvement of DNA vaccines. The most frequently used vectors for genetic immunization are plasmids. In this article, we review some of the main elements relevant to their design such as strong promoter/enhancer region, introns, genes encoding antigens of interest from the pathogen (how to choose and modify them), polyadenylation termination sequence, origin of replication for plasmid production in Escherichia coli, antibiotic resistance gene as selectable marker,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic immunization; DNA plasmid; Enhancer/promoter region; Immunostimulatory sequences (ISS); Antigens; Immune response. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200002 |
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