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| Sá,Aline de Jesus; Lédo,Ana da Silva; Lédo,Carlos Alberto da Silva; Pasqual,Moacir; Silva,Ana Veruska Cruz da; Silva Junior,Josué Francisco da. |
| In micropropagation, especially for mangaba tree botanical variety of Northeastern Brazil, limiting aspects such as ethylene accumulation in the cultivation flask and loss of vigor in subcultures have been observed. This study was aimed at assessing the technical and scientific knowledge of the in vitro propagation of botanical mangaba tree variety and at improving the micropropagation protocol, establishing the in vitro cultivation time, the best type of flask sealing and explant at different micropropagation stages. For the establishment phase and for the first and second subcultures, the MS medium with 3% sucrose and 0.6% agar, supplemented with 1 mg L-1 IAA and 1 mg L-1 BA was used. Evaluations were performed at 30, 50 and 65 days of in vitro... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hancornia speciosa; Tissue culture; In vitro ethylene; Nodal segments. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000400004 |
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