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| Ferraz,Rafaela Siqueira; Mendonça,Elisângela Afonso Moura; Silva,Jéssica Priscila Avelino; Cavalcanti,Isabella Macário Ferro; Lira-Nogueira,Mariane Cajubá Britto; Galdino,Suely Lins; Pitta,Ivan Rocha; Lima,Maria do Carmo Alves; Santos-Magalhães,Nereide Stela. |
| The aim of this study was to develop and validate a UV spectrophotometric method for determination of LPSF/AC04 from inclusion complex and encapsulated into liposomes. The validation parameters were determined according to the International Conference on Harmonisation (ICH) and National Health Surveillance Agency (ANVISA) guidelines. LPSF/AC04 was determined at 250 nm in methanol by a UV spectrophotometric method, exhibiting linearity in the range from 0.3 to 2 µg.mL−1 (Absorbance=0.18068 x [LPSF/AC04 µg.mL-1] + 0.00348), (r2=0.9995). The limits of detection and quantification were 0.047µg.mL−1 and 0.143µg.mL−1, respectively. The method was accurate, precise, reproducible and robust since all the samples analyzed had coefficient of variation of less than... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: UV Spectrophotometry/quantitative analysis/method validation; Acridine derivatives/determination; Inclusion complex; Liposomes. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100183 |
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