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| OHARA,Andre; SANTOS,Jessika Gonçalves dos; ANGELOTTI,Joelise Alencar Figueira; BARBOSA,Paula de Paula Menezes; DIAS,Fernanda Furlan Gonçalves; BAGAGLI,Marcela Pavan; SATO,Hélia Harumi; CASTRO,Ruann Janser Soares de. |
| Abstract This study reports the use of statistical mixture design as a tool for the simultaneous production of lipase, CMCase, α-amylase, and β-glucosidase by Aspergillus niger under solid-state fermentation. Wheat bran, soybean meal, cottonseed meal, and orange peel were used as substrates, either individually or combined in different formulations, to study their synergistic or antagonistic effects on production of the enzymes. The highest lipase (323 U g-1) and CMCase (10 U g-1) activities were detected after 48 h, while the maximum activities of α-amylase (18 U g-1) and β-glucosidase (15 U g-1) occurred at 72 and 96 h, respectively. Considering the substrate formulation, the ternary mixture of wheat bran (1/3), soybean meal (1/3), and cottonseed meal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Industrial enzymes; Solid-state fermentation; Aspergillus niger; Agroindustrial wastes; Mixture design. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612018000500131 |
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| Garcia,Nayara Fernanda Lisboa; Santos,Flávia Regina da Silva; Gonçalves,Fabiano Avelino; Paz,Marcelo Fossa da; Fonseca,Gustavo Graciano; Leite,Rodrigo Simões Ribeiro. |
| Background β-Glucosidases catalyze the hydrolysis of cellobiose and cellodextrins, releasing glucose as the main product. This enzyme is used in the food, pharmaceutical, and biofuel industries. The aim of this work is to improve the β-glucosidase production by the fungus Lichtheimia ramosa by solid-state fermentation (SSF) using various agroindustrial residues and to evaluate the catalytic properties of this enzyme. Results A high production of β-glucosidase, about 274 U/g of dry substrate (or 27.4 U/mL), was obtained by cultivating the fungus on wheat bran with 65% of initial substrate moisture, at 96 h of incubation at 35°C. The enzymatic extract also exhibited carboxymethylcellulase (CMCase), xylanase, and... |
| Tipo: Journal article |
Palavras-chave: Cellobiase; Cellulases and hemicellulases; Industrial enzymes; Microbial enzymes. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000400010 |
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