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	Provedor de dados Anais da ABC (AABC) (1) BJM (1) Autor Arenhart,Sandra (1) BERTANI,GIOVANI R. (1) CORDEIRO,MARLI T. (1) Flores,Eduardo Furtado (1) GIL,LAURA H.V.G. (1) Gil,Laura Helena Vega Gonzales (1) MARQUES,ERNESTO T.A. (1) SANTOS,JEFFERSON J.S. (1) Silva Junior,José Valter Joaquim (1) Weiblen,Rudi (1) Mais... Palavra-chave Infectious clone (2) Reverse genetics (2) BVDV (1) Dengue virus (1) Molecular cloning (1) Pestivirus (1) Yeast homologous recombination (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2016 (1) 2014 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última A two-plasmid strategy for engineering a dengue virus type 3 infectious clone from primary Brazilian isolate Anais da ABC (AABC) SANTOS,JEFFERSON J.S.; CORDEIRO,MARLI T.; BERTANI,GIOVANI R.; MARQUES,ERNESTO T.A.; GIL,LAURA H.V.G.. Dengue infections represent one of the most prevalent arthropod-borne diseases worldwide, causing a wide spectrum of clinical outcomes. Engineered infectious clone is an important tool to study Dengue virus (DENV) biology. Functional full-length cDNA clones have been constructed for many positive-strand RNA viruses and have provided valuable tools for studying the molecular mechanisms involved in viral genome replication, virion assembly, virus pathogenesis and vaccine development. We report herein the successful development of an infectious clone from a primary Brazilian isolate of dengue virus 3 (DENV3) of the genotype III. Using a two-plasmid strategy, DENV3 genome was divided in two parts and cloned separately into a yeast-bacteria shuttle vector. All... Tipo: Info:eu-repo/semantics/article Palavras-chave: Reverse genetics; Dengue virus; Molecular cloning; Infectious clone. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401749 Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones BJM Arenhart,Sandra; Silva Junior,José Valter Joaquim; Flores,Eduardo Furtado; Weiblen,Rudi; Gil,Laura Helena Vega Gonzales. Abstract The open reading frame of a Brazilian bovine viral diarrhea virus (BVDV) strain, IBSP4ncp, was recombined with the untranslated regions of the reference NADL strain by homologous recombination in Saccharomyces cerevisiae, resulting in chimeric full-length cDNA clones of BVDV (chi-NADL/IBSP4ncp#2 and chi-NADL/IBSP4ncp#3). The recombinant clones were successfully recovered, resulting in viable viruses, having the kinetics of replication, focus size, and morphology similar to those of the parental virus, IBSP4ncp. In addition, the chimeric viruses remained stable for at least 10 passages in cell culture, maintaining their replication efficiency unaltered. Nucleotide sequencing revealed a few point mutations; nevertheless, the phenotype of the rescued... Tipo: Info:eu-repo/semantics/article Palavras-chave: BVDV; Pestivirus; Reverse genetics; Infectious clone; Yeast homologous recombination. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400993 Registros recuperados: 2 Primeira ... 1 ... Última

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