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THE IMPORTANCE OF ANTIMICROBIAL COMPOUNDS PRODUCED BY BENEFICIAL BACTERIA ON THE BIOCONTROL OF PHYTOPATHOGENS Acta biol.Colomb.
CESA-LUNA,Catherine; BAEZ,Antonino; QUINTERO-HERNÁNDEZ,Verónica; CRUZ-ENRÍQUEZ,Joel DE LA; CASTAÑEDA-ANTONIO,Ma Dolores; MUÑOZ-ROJAS,Jesús.
ABSTRACT Bacteria produce antimicrobial compounds to compete for nutrients and space in a particular habitat. Antagonistic interactions can be evaluated by several methodologies including the double-layer agar and simultaneous inhibition assays. Among the well-known inhibitory substances produced by bacteria are the broad-spectrum antibiotics, organic acids, siderophores, antifungal, and bacteriocins. The most studied bacterial genera able to produce these inhibitory substances are Enterococcus, Lactococcus, Streptomyces, Bacillus, Pseudomonas, Klebsiella, Escherichia, and Burkholderia. Some beneficial bacteria can promote plant growth and degrade toxic compounds in the environment representing an attractive solution to diverse issues in agriculture and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antagonism; Antibiotic; Competition; Inhibition; PGPR.
Ano: 2020 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2020000100140
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Método de difusión lumínica aplicado a la capacidad inhibitoria para epitopes ABO en Ascaris lumbricoides ABCL
Ponce de León,Patricia; Foresto,Patricia; Valverde,Juana.
Experiencias previas demostraron la presencia de epitopes ABO en Ascaris lumbricoides. El objetivo de este trabajo fue determinar la capacidad inhibitoria del parásito para estos epitopes por cuantificación de la hemaglutinación aplicando el método de difusión lumínica por partículas en suspensión. Se seleccionaron 8 extractos parasitarios (EA) de pacientes grupo sanguíneo B y 4 de grupo A (con y sin epitopes ABO). Los EA fueron enfrentados a diluciones de antisuero monoclonal (anti A o anti B) y el antisuero excedente fue neutralizado con eritrocitos de isogrupo. Se midió la extinción óptica de la aglutinación resultante. Se graficó el porcentaje de aglutinación en función de la dilución del antisuero para calcular Título-50 (T-50). Se definió Poder...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ascaris lumbricoides; Sistema de grupo sanguíneo ABO; Capacidad inhibitoria; Difusión lumínica por partículas en suspensión; Ascaris lumbricoides; Inhibition.
Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572005000400008
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Allelopathic potential of bark and leaves of Esenbeckia leiocarpa Engl. (Rutaceae) Acta Botanica
Souza,Flaviana Maluf; Gandolfi,Sergius; Perez,Sonia Cristina Juliano Gualtieri de Andrade; Rodrigues,Ricardo Ribeiro.
We investigated the inhibitory potential of aqueous extracts of bark and leaves of Esenbeckia leiocarpa Engl. on lettuce germination and early seedling growth. We compared the effects of four concentrations (100, 75, 50 and 25%) of each extract to water and polyethylene glycol (PEG 6000) solution controls for four replicates of 50 seeds for germination and four replicates of ten seedlings for seedling growth. The inhibitory effects of E. leiocarpa extracts on the percentage of germination and on the germination speed seemed to be more than simply an osmotic effect, except for the percentage of seeds germinated in bark extracts. When compared to water control, both bark and leaf extracts delayed germination, and leaf extracts also affected the percentage of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allelopathy; Germination; Growth; Inhibition; Plant-plant interaction.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062010000100016
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Use of wild yeasts as a biocontrol agent against toxigenic fungi and OTA production Agronomy
Souza, Mariana Lino; Passamani, Fabiana Reinis Franca; Ávila, Carla Luiza da Silva; Batista, Luis Roberto; Schwan, Rosane Freitas; Silva, Cristina Ferreira.
This study evaluated the antagonistic potential of 32 wild yeast isolates from coffee and cocoa bean fermentation. These yeasts were inoculated in co-cultivation with Aspergillus carbonarius (CCDCA 10608 and CCDCA 10408) and Aspergillus ochraceus (CCDCA 10612) isolated from grapes and coffee beans. The mycelial growth and ochratoxin A (OTA) production were evaluated, and the spores were counted after cultivation at 28°C for seven days. The yeasts presented higher inhibitory effects (53% in relation to the control) over the mycelial growth of the isolated A. ochraceus (CCDCA10612). Pichia anomala CCMA0148 and Saccharomyces cerevisiae CCMA0159 provided the greatest inhibition of the growth of all fungal strains. All Pichia species presented the highest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.01.02.04-4 antagonistic activity; Ochratoxin A; Aspergillus; Inhibition; Pichia sp.; Saccharomyces sp Fitosanidade Microbiologia Agricola.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/32659
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Phytotoxic potential of Drimys brasiliensis Miers for use in weed control Agronomy
Anese, Simoni; Gualtieri, Sonia Cristina Juliano; Grisi, Patrícia Umeda; Jatobá, Luciana de Jesus; Arduin, Marcos.
This study aims to evaluate the phytotoxicity potential of leaf and root extracts of Drimys brasiliensis on the germination and seedling growth of Panicum maximum and Euphorbia heterophylla and its influence on metaxylem cell size in the seedling roots of the latter specie. The leaf and root extracts were fractionated by partition chromatography, and the hexane and ethyl acetate fractions obtained from each organ were evaluated at different concentrations for phytotoxic activity in several bioassays. In seedling growth tests, we compared the effects of these fractions with the herbicide oxyfluorfen. The hexane fraction of the root extracts showed a higher inhibitory potential on the germination and growth of weeds and reduced the average size of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agronomia - Fisiologia vegetal allelopathy; Inhibition; Germination; Early growth. Controle de plantas daninhas.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19476
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The anti-inflammatory activity of standard aqueous stem bark extract of Mangifera indica L. as evident in inhibition of Group IA sPLA2 Anais da ABC (AABC)
DHANANJAYA,BHADRAPURA LAKKAPPA; SHIVALINGAIAH,SUDHARSHAN.
ABSTRACT The standard aqueous stem bark extract is consumed as herbal drink and used in the pharmaceutical formulations to treat patients suffering from various disease conditions in Cuba. This study was carried out to evaluate the modulatory effect of standard aqueous bark extract of M. indica on Group IA sPLA2. M. indica extract, dose dependently inhibited the GIA sPLA2 (NN-XIa-PLA2) activity with an IC50 value 8.1 µg/ml. M. indica extract effectively inhibited the indirect hemolytic activity up to 98% at ~40 µg/ml concentration and at various concentrations (0-50 µg/ml), it dose dependently inhibited the edema formation. When examined as a function of increased substrate and calcium concentration, there was no relieve of inhibitory effect on the GIA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Secretory phospholipase A2; Anti-inflammatory; Pro-inflammatory; Inhibition; Drug; Plants.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100197
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Activation of group II metabotropic glutamate receptors blocks zinc release from hippocampal mossy fibers Biol. Res.
Matias,Carlos M; Dionísio,Jose C; Saggau,Peter; Quinta-Ferreira,Maria Emilia.
BACKGROUND: The hippocampal CA3 area contains large amounts of vesicular zinc in the mossy fiber terminals which is released during synaptic activity, depending on presynaptic calcium. Another characteristic of these synapses is the presynaptic localization of high concentrations of group II metabotropic glutamate receptors, specifically activated by DCG-IV. Previous work has shown that DCG-IV affects only mossy fiber-evoked responses but not the signals from associational-commissural afferents, blocking mossy fiber synaptic transmission. Since zinc is released from mossy fibers even for single stimuli and it is generally assumed to be co-released with glutamate, the aim of the work was to investigate the effect of DCG-IV on mossy fiber zinc signals....
Tipo: Journal article Palavras-chave: Zinc; Neurotransmission; Glutamatergic; Co-release; Metabotropic fluorescence; Inhibition.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100067
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Production of functional killer protein in batch cultures upon a shift from aerobic to anaerobic conditions BABT
Silva,Gildo Almeida da; Poli,Jandora Severo; Poletto,Carolina Madalozzo; Schaker,Patricia Dayane Carvalho; Valente,Patricia.
The aim of this work was to study the production of functional protein in yeast culture. The cells of Saccharomyces cerevisiae Embrapa 1B (K+R+) killed a strain of Saccharomyces cerevisiae Embrapa 26B (K-R-)in grape must and YEPD media. The lethal effect of toxin-containing supernatant and the effect of aeration upon functional killer production and the correlation between the products of anaerobic metabolism and the functional toxin formation were evaluated. The results showed that at low sugar concentration, the toxin of the killer strain of Sacch. cerevisiae was only produced under anaerobic conditions . The system of killer protein production showed to be regulated by Pasteur and Crabtree effects. As soon as the ethanol was formed, the functional...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Killer yeast; Crabtree effect; Pasteur effect; Inhibition; Mycocinogenic strain.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300022
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Growth inhibition of Staphylococcus aureus and escherichia coli strains by neutralizing IgY antibodies from ostrich egg yolk BJM
Tobias,Fernando Luiz; Garcia,Luize Néli Nunes; Kanashiro,Milton Masahiko; Medina-Acosta,Enrique; Brom-de-Luna,João Gato; Almeida,Claudia Maria Costa de; Azevedo Junior,Romildo Rocha; Lemos,Môsar; Vieira-da-Motta,Olney.
Ostrich raising around the world have some key factors and farming profit depend largely on information and ability of farmers to rear these animals. Non fertilized eggs from ostriches are discharged in the reproduction season. Staphylococcus aureus and Escherichia coli are microorganisms involved in animal and human diseases. In order to optimize the use of sub products of ostrich raising, non fertilized eggs of four selected birds were utilized for development of polyclonal IgY antibodies. The birds were immunized (200ug/animal) with purified recombinant staphylococcal enterotoxin C (recSEC) and synthetic recRAP, both derived from S. aureus, and recBFPA and recEspB involved in E. coli pathogenicity, diluted in FCA injected in the braquial muscle. Two...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ostrich IgY antibodies; Inhibition; Recombinant proteins; E. coli; S. aureus.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200015
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Strains of Lentinula edodes suppress growth of phytopathogenic fungi and inhibit Alagoas serotype of vesicular stomatitis virus BJM
Sasaki,Selma H.; Linhares,Rosa E.C.; Nozawa,Carlos M.; Montalván,Ricardo; Paccola-Meirelles,Luzia D..
Four Lentinula edodes strains (Le10, 46, K2, Assai) were assessed for their antagonistic effect on four filamentous fungus species of agricultural importance (Helminthosporium euphorbiae, Helminthosporium sp, Fusarium solani and Phomopsis sojae) and on Alagoas serotype of Vesicular Stomatitis Virus (VSA). The L. edodes strains studied had variable effects on the filamentous fungi and on VSA. The K2 and Le10 strains were antagonistic on the fungi assessed and the 46 and K2 strains were efficient on the Vesicular Stomatitis Virus. The results widened the list of beneficial effects of L. edodes on the control and prevention of animal pathogenic virus and filamentous fungi.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phytopathogenic fungi; Shiitake; Inhibition; Vesicular Stomatitis Virus (Alagoas).
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100012
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Hesperidin inhibits the epithelial to mesenchymal transition induced by transforming growth factor-β1 in A549 cells through Smad signaling in the cytoplasm BJPS
Ren,Zhouxin; Shen,Junling; Mei,Xiaofeng; Dong,Haoran; Li,Jiansheng; Yu,Haibin.
Hesperidin, a natural compound, suppresses the epithelial-to-mesenchymal transition through the TGF-β1/Smad signaling pathway. However, studies on the detailed effects and mechanisms of hesperidin are rare. The present study showed that, for A549 alveolar epithelial cells, the anti-proliferative effects of hesperidin occurred in a dose-dependent manner, with an IC50= 216.8 µM at 48 h. TGF-β1 was used to activate the Smad signaling pathway and induce the epithelial to mesenchymal transition in cells. Treatment with hesperidin or SB431542 was used for antagonism of Smad pathway activation. Hesperidin inhibited the increase in ɑ-SMA and Col1ɑ-1 and the decrease in E-cadherin in a dose-dependent manner from concentration of 20 µM to 60 µM, as assessed by both...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hesperidin; Epithelial-to-mesenchymal transition (EMT); Smad signaling pathway; Inhibition.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100566
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Effects of fermentation substrates and conservation methods on the viability and antimicrobial activity of Weissella confusa and its metabolites Electron. J. Biotechnol.
Serna-Cock,Liliana; Enríquez-Valencia,Cruz E; Jiménez-Obando,Eliana M; Campos-Gaona,Rómulo.
Lactic acid bacteria produce metabolites with antagonistic activity against other bacteria. However, growth conditions and conservation methods may reduce the viability and antimicrobial activity of lactic acid bacteria. This study evaluated the effects of fermentation substrate, lyophilization (freeze-drying) and refrigeration on the viability and antimicrobial activity of Weissella confusa strain and its metabolites against pathogens responsible for bovine mastitis. W. confusa strain was grown in MRS broth and milk supplemented with yeast extract and glucose (MYEG). The collected fractions were preserved by lyophilization or under refrigeration at 4ºC. Every seven days, the viability of W. confusa strain and the stability of its metabolites were...
Tipo: Journal article Palavras-chave: Conservation; Inhibition; Lactic acid bacteria; Lyophilization.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300006
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Guava Psidium guajava seed flour and dry Aspergillus niger mycelium as nitrogen sources for the production of biomass and antimicrobial compounds produced by Weissella confusa Electron. J. Biotechnol.
Serna-Cock,Liliana; Mera-Ayala,José Daniel; Angulo-López,Jorge Eduardo.
Background: The fermentation substrate efficiency of glucose supplemented with guava seed flour (GGSF) or glucose supplemented with dry Aspergillus niger mycelium (GANM) was evaluated during the production of biomass and antimicrobial compounds by the lactic acid bacteria Weissella confusa. Results: The fermentation substrate efficiency was measured by comparing the biomass formation, substrate consumption, substrate conversion, antimicrobial activity and product yield. The antimicrobial activity was measured against a commercial Staphylococcus aureus strain. The results were compared against fermentations performed in a commercial substrate (CS), the MRS (Man-Rogosa-Sharpe) substrate. The fermentations were performed discontinuously for 4 hrs at 100 rpm...
Tipo: Journal article Palavras-chave: Agroindustrial residue; Bacteriocin; Biomass; Inhibition.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600017
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Selection of psychotrophic bacteria active against spoilage and pathogenic micro-organisms relevant for seafood products ArchiMer
Matamoros, Sébastien; Pilet, Marie-france; Gigout, Frédérique; Prevost, Hervé; Leroi, Françoise.
In this study, 51 seafood products were screened to select psychrophilic inhibiting lactic acid bacteria. 5575 colonies were tested for inhibition against four target strains : Listeria monocytogenes, Staphylococcus xylosus, Pseudomonas sp. and Serratia liquefaciens. 456 colonies (8,2%) showed inhibition and 132 (28,9%) of them were picked up. 54 isolates growing at 15°C but not at 30°C were selected. Basic phenotypic characteristics (Gram, catalase and oxidase test) were then tested. Finally, 52 presumptive psychrophilic LAB strains were selected. The inhibition spectrum of these strains was enlarged to 14 spoiling or pathogenic target strains relevant for seafood products. The inhibition profiles were recorded, and clustering was made to separate eight...
Tipo: Text Palavras-chave: Biopreservation; Seafood products; Psychrophilic lactic acid bacteria; Inhibition.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/00002/11339/7888.pdf
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Selection and evaluation of seafood-borne psychrotrophic lactic acid bacteria as inhibitors of pathogenic and spoilage bacteria ArchiMer
Matamoros, Sebastien; Pilet, Marie-france; Gigout, Frederique; Prevost, Herve; Leroi, Francoise.
In this study, inhibitory psychrotrophic lactic acid bacteria were isolated and investigated for future use in biopreservation of seafood products. Screening of 5575 colonies isolated from various seafood products resulted in the selection of 132 colonies presenting inhibitory properties. Among them, 52 isolates had characteristics of LAB and showed growth at 15 °C but not at 30 °C. The inhibition spectrum of these 52 isolates against 14 target strains (Gram-positive and -negative) showed inhibition of typical seafood spoiling and pathogenic bacteria and enabled the formation of seven interesting clusters. Sequencing of the 16S rRNA gene of a representative isolate from each cluster identified three Leuconostoc gelidum, two Lactococcus piscium, one...
Tipo: Text Palavras-chave: Carnobacterium alterfunditum; Lactobacillus fuchuensis; Leuconostoc gelidum; Lactococcus piscium; Psychrotrophic; Inhibition; Seafood; Lactic acid bacteria.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6472.pdf
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Formaldehyde preservation for deferred measurements of alkaline phosphatase activities in marine samples ArchiMer
Labry, Claire; Urvoy, Marion.
Alkaline phosphatases are the main enzymes required by microorganisms to hydrolyse organic phosphorus into available phosphate in aquatic environments. The investigations of alkaline phosphatase activity (APA) usually generate numerous samples (size fractionation, Michaelis-Menten kinetics). Therefore, convenient and reliable preservation of incubated samples for a deferred analysis would be very useful when measurements cannot be performed right away. The APA of marine pond waters was measured using 4-Methylumbelliferyl phosphate (MUF-P) as the fluorogenic substrate modelling natural organic phosphorus compounds. Where typical inhibitors of other enzymatic activities, such as 1% sodium dodecyl sulfate, mercuric chloride, or buffered solutions of ammonium...
Tipo: Text Palavras-chave: Alkaline phosphatase activity; Formaldehyde; Inhibition; Enzymes; Aquatic studies; Deferred measurements.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00656/76787/78211.pdf
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Protective Effect of a Non-Bacteriocinogenic Lactococcus piscium CNCM I-4031 Strain Against Listeria monocytogenes in Sterilized Tropical Cooked Peeled Shrimp ArchiMer
Fall, Papa-abdoulaye; Leroi, Francoise; Chevalier, Frederique; Guerin, Charline; Pilet, Marie-france.
The protective activity of a non-bacteriocinogenic Lactococcus piscium CNCM I-4031 strain against Listeria monocytogenes was investigated in tropical cooked peeled shrimp stored at 8°C in modified atmosphere packaging (50% N2-50% CO2). When inoculated alone (L. piscium 107 CFU g-1 and L. monocytogenes 104 CFU g-1), protective culture and target strain grew very well on shrimp reaching a maximum cell number of 109 CFU g-1 after 7 and 14 days, respectively. In the presence of L. piscium, growth of L. monocytogenes was totally prevented after 3 days of storage. The count was 3.4 log CFU g-1 lower than in the control after 10 days and until the end of storage (31 days). Using the Seafood Spoilage and Safety Predictor Software (http://sssp.dtuaqua.dk), it was...
Tipo: Text Palavras-chave: Inhibition; Biopreservation; Challenge-test; Lactic acid; Nutritional competition.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00005/11624/8281.pdf
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Études des interactions entre la bactérie bioprotectrice Lactococcus piscium et Brochothrix thermosphacta et Listeria monocytogenes dans la crevette tropicale ArchiMer
Fall, Papa-abdoulaye.
The bioprotective potential of Lactococcus piscium CNCM I-4031 towards a specific spoilage organism, Brochothrix thermosphacta, was studied on tropical cooked peeled shrimp packed under modified atmosphere. In co-culture, the growth of this spoilage bacterium was inhibited by 4 log ufc/g by L. piscium and the sensory quality of the product was improved during 31 days. The inhibition could not be attributed to lactic acid or nutritional competition for amino acids. The growth modelling of L. piscium and B. thermosphacta was performed in different conditions of temperature, pH and NaCl concentrations. The optimal growth values of these two bacteria are quite similar, however L. piscium seemed to be more psychrotolerant than B. thermosphacta but twice less...
Tipo: Text Palavras-chave: Biopreservation; Inhibition; Modélisation; Lactococcus piscium; Flore d’altération; Analyse sensorielle; Produits de la mer; Co-cultures; Biopreservation; Inhibition; Modelling; Lactococcus piscium; Spoilage microflora; Sensory analysis; Seafood products; Co-cultures.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00050/16164/13649.pdf
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Enzymatic and biochemical characterization of Bungarus sindanus snake venom acetylcholinesterase J. Venom. Anim. Toxins incl. Trop. Dis.
Ahmed,M; Latif,N; Khan,RA; Ahmad,A; Rocha,JBT; Mazzanti,CM; Bagatini,MD; Morsch,VM; Schetinger,MRC.
This study analyses venom from the elapid krait snake Bungarus sindanus, which contains a high level of acetylcholinesterase (AChE) activity. The enzyme showed optimum activity at alkaline pH (8.5) and 45ºC. Krait venom AChE was inhibited by substrate. Inhibition was significantly reduced by using a high ionic strength buffer; low ionic strength buffer (10 mM PO4 pH 7.5) inhibited the enzyme by 1. 5mM AcSCh, while high ionic strength buffer (62 mM PO4 pH 7.5) inhibited it by 1 mM AcSCh. Venom acetylcholinesterase was also found to be thermally stable at 45ºC; it only lost 5% of its activity after incubation at 45ºC for 40 minutes. The Michaelis-Menten constant (Km) for acetylthiocholine iodide hydrolysis was found to be 0.068 mM. Krait venom...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acetylcholinesterase; Inhibition; Krait; Ionic strength; Acetylthiocholine iodide; Bungarus sindanus; Snake venom.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200014
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Inhibición de los contaminantes del tempeh de soya OceanDocs
Mejías, E.; Delgado, R.; Silveira, I.; Lafargue, D.; Cárdenas, E..
Se evaluó el efecto de diferentes especias o condimentos sobre la calidad microbiológica del tempeh y la posible acción inhibidora de sus contaminantes. El tempeh se obtuvo a partir de frijol de soya el cual se sometió a los procesos de cocción, escurrimiento y fermentación. A continuación se procedió a incorporar los condimentos ensayados en dos proporciones por separado a la soya fresca antes de su inoculación con la cepa R. oligosporus, se mezclaron bien y se sometieron al proceso de fermentación, el cual tuvo lugar en bandejas de poliestireno, incubadas a 30 ± 2 ºC por un período de 18 a 20 h hasta obtener un producto con las características típicas del tempeh. De éste se tomaron muestras para determinar contenido de humedad, conteo total de viables y...
Tipo: Journal Contribution Palavras-chave: Biological contamination; Chemical activity; Tempeh; Bacterial contamination; Enterobacteriaceae; Inhibition; Garlic; Anions; Spices.
Ano: 2008 URL: http://hdl.handle.net/1834/5010
Registros recuperados: 24
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