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	Provedor de dados BJMBR (2) Electron. J. Biotechnol. (1) Autor Alito,A. (1) Batista,Fabiana R. X. (1) Bigi,F. (1) Cataldi,A.A. (1) Fisanotti,J.C. (1) Mendonça,Ronaldo Z. (1) Miranda,E.A. (1) Moraes,Angela M. (1) Ménissier-de-Murcia,J. (1) Pereira,Carlos A. (1) Romano,M.I. (1) Taboga,O. (1) de-Murcia,G. (1) Mais... Palavra-chave Baculovirus (3) Insect cells (3) Antigen (1) Concentrated milk whey (1) Grace's medium (1) MPB70 (1) Media formulation (1) Mycobacterium smegmatis (1) Mycobacterium tuberculosis (1) Overproduction (1) P36 (1) PARP (1) Secreted proteins (1) Spodoptera frugiperda Sf9 (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Journal article (1) Ano 2006 (1) 1999 (1) 1997 (1) País Brazil (2) Chile (1) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Expression of the Mycobacterium bovis P36 gene in Mycobacterium smegmatis and the baculovirus/insect cell system BJMBR Bigi,F.; Taboga,O.; Romano,M.I.; Alito,A.; Fisanotti,J.C.; Cataldi,A.A.. In the present study we evaluated different systems for the expression of mycobacterial antigen P36 secreted by Mycobacterium bovis. P36 was detected by Western blot using a specific antiserum. The P36 gene was initially expressed in E. coli, under the control of the T7 promoter, but severe proteolysis prevented its purification. We then tried to express P36 in M. smegmatis and insect cells. For M. smegmatis, we used three different plasmid vectors differing in copy number and in the presence of a promoter for expression of heterologous proteins. P36 was detected in the cell extract and culture supernatant in both expression systems and was recognized by sera from M. bovis-infected cattle. To compare the expression level and compartmentalization, the MPB70... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium tuberculosis; Mycobacterium smegmatis; Insect cells; Baculovirus; Secreted proteins; P36; MPB70; Antigen. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100004 Large-scale production and purification of recombinant protein from an insect cell/baculovirus system in Erlenmeyer flasks: application to the chicken poly(ADP-ribose) polymerase catalytic domain BJMBR Miranda,E.A.; de-Murcia,G.; Ménissier-de-Murcia,J.. A simple and inexpensive shaker/Erlenmeyer flask system for large-scale cultivation of insect cells is described and compared to a commercial spinner system. On the basis of maximum cell density, average population doubling time and overproduction of recombinant protein, a better result was obtained with a simpler and less expensive bioreactor consisting of Erlenmeyer flasks and an ordinary shaker waterbath. Routinely, about 90 mg of pure poly(ADP-ribose) polymerase catalytic domain was obtained for a total of 3 x 109 infected cells in three liters of culture Tipo: Info:eu-repo/semantics/article Palavras-chave: PARP; Insect cells; Baculovirus; Overproduction. Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800002 Evaluation of concentrated milk whey as a supplement for SF9 Spodoptera frugiperda cells in culture Electron. J. Biotechnol. Batista,Fabiana R. X.; Pereira,Carlos A.; Mendonça,Ronaldo Z.; Moraes,Angela M.. Insect cell culture has become increasingly useful for the production of heterologous proteins as well as of baculovirus polyhedra, and several different culture media formulations can be employed for this purpose. The goal of this work was to assess the potential of lyophylized milk whey ultra filtration retentate (MWR) when associated to yeast extract (YE), glucose and Pluronic F68 (PF68) to partially replace fetal calf serum (FBS) in Spodoptera frugiperda Sf9 cells culture in Grace's medium, aiming AgMNPV baculovirus production. Batch cultivation results showed that the yeast extract and the milk whey concentrate effectively increased cell concentration to about half the level commonly verified for the Sf900II serum-free medium. A 4-fold increase in... Tipo: Journal article Palavras-chave: Baculovirus; Concentrated milk whey; Grace's medium; Insect cells; Media formulation; Spodoptera frugiperda Sf9. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500007 Registros recuperados: 3 Primeira ... 1 ... Última

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