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Effect of Baculovirus spodoptera isolates in Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) larvae and their characterization by RAPD Neotropical Entomology
Barreto,Marliton R.; Guimaraes,Cláudia T.; Teixeira,Flávia F.; Paiva,Edilson; Valicente,Fernando H..
The total of 22 Baculovirus isolates surveyed in different corn producing regions in Brazil were used against fall armyworm, Spodoptera frugiperda (J.E. Smith). The viruses were purified and their suspensions were used to feed fall armyworm larvae from 4th and 5th instar. The mortality rate was checked daily and the infected larvae were frost after death, what generally occurred between the 5th and 7th day after virus ingestion. The 22 Baculovirus isolates were used in six concentrations (from 10³ to 10(8) polyhedra/ml) and one check treatment with water. Mortality rate, larval period, pupal period, pupa weight and lethal concentration (LC50) were determined for all isolates. Significant differences were found among all isolates and different...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insect pathology; Fall armyworm larvae; PCR.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000100010
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Selection of Native Fungi Strains Pathogenic to Vespula germanica (Hymenoptera: Vespidae) Agricultura Técnica
Merino,Loreto; France,Andrés; Gerding,Marcos.
The yellowjacket wasp, Vespula germanica F., is considered a serious pest of productive and recreational activities worldwide. A pathogenicity study was carried out with 29 strains of Metarhizium anisopliae and 30 of Beauveria bassiana against worker and male wasps. Wasps of the same age were fed with liquid sugar baits containing 1 x 10(8) conidia mL-1 suspensions of each strain. The highest mortality and sporulation were obtained with the strains Qu-B941 and Qu-B933 of Beauveria bassiana, reaching 79 and 95% mortality for workers and 66 and 73% for males, respectively. The strains were tested on workers of V. germanica with increasing amounts of bait from 0 to 1 x 10(8) conidia mL-1. The results also showed that 1 x 10(8) conidia mL-1 increased up to 90...
Tipo: Journal article Palavras-chave: Entomopathogens; Insect pathology; Biological control; Yellow jacket.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072007000400001
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Evaluation of Metarhizium anisopliae var. anisopliae Qu-M845 Isolate to Control Varroa destructor (Acari: Varroidae) in Laboratory and Field Trials Chilean J. Agric. Res.
Rodríguez,Marta; Gerding,Marcos; France,Andrés; Ceballos,Ricardo.
The effectiveness of the Metarhizium anisopliae (Metschinkoff) Qu-M845 isolate was evaluated in laboratory and field trials. It was previously selected for thermal resistance (at 30 and 35 ºC) and pathogenicity on Varroa destructor (Anderson and Trueman). In the laboratory, the first evaluations were carried out by spraying and increasing the concentration from zero to 10(8) conidia mL-1 on varroa adults. Lethal concentrations required for 50 and 90% mite mortality (LC50 and LC90) were 3.8 x 10(5) and 8 x 10(7) conidia mL-1, respectively (χ2 = 2.03). In the autumn field trials, three application methods (doses of 5 x 10(10) conidia per hive) were evaluated. The treatments were: a) conidia stamped on filter paper, located on every second frame...
Tipo: Journal article Palavras-chave: Biological control; Entomopathogenic fungi; Varroasis; Insect pathology.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000400009
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