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	Provedor de dados BJMBR (2) Electron. J. Biotechnol. (1) Autor Costanzi-Strauss,E. (1) Mohan Prasad,Anand (1) Paçó-Larson,M.L. (1) Pereira,G.B. (1) Radhamony,Resmi Nath (1) Srinivasan,Ramamurthy (1) Strauss,B.E. (1) Valente,V. (1) Vianna,M.C.B. (1) de Queiroz,M.S. (1) Mais... Palavra-chave Insertional mutagenesis (3) Aberrant splicing (1) Adverse event (1) Arabidopsis (1) Clinical trial (1) DmLpin (1) Functional genomics (1) Gene therapy (1) Kermit/dGIPC (1) Promoter trapping (1) Retrovirus (1) Severe combined immune deficiency (1) T-DNA tagging (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Journal article (1) Ano 2011 (1) 2007 (1) 2005 (1) País Brazil (2) Chile (1) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última T-DNA insertional mutagenesis in Arabidopsis: a tool for functional genomics Electron. J. Biotechnol. Radhamony,Resmi Nath; Mohan Prasad,Anand; Srinivasan,Ramamurthy. With the availability of complete genome sequences of several organisms, the focus has shifted from structural genomics to functional genomics, specifically in plants where the complete genomic sequences are becoming available i.e., Arabidopsis and rice. Agrobacterium mediated transformation which is exploited for transgenic technology is also being used as an effective mutagen and as a tool for functional genomics in higher plants. Besides the fact that the insertion of T-DNA element into a gene can lead to loss or gain of function, ingenious use of a variety of vectors have led to the identification of genes and regulatory elements in Arabidopsis. In this review, we highlight the progress made in the field of functional genomics of Arabidopsis using... Tipo: Journal article Palavras-chave: Arabidopsis; Functional genomics; Insertional mutagenesis; Promoter trapping; T-DNA tagging. Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100012 Combating oncogene activation associated with retrovirus-mediated gene therapy of X-linked severe combined immunodeficiency BJMBR Strauss,B.E.; Costanzi-Strauss,E.. A successful gene therapy clinical trial that also encountered serious adverse effects has sparked extensive study and debate about the future directions for retrovirus-mediated interventions. Treatment of X-linked severe combined immunodeficiency with an oncoretrovirus harboring a normal copy of the gc gene was applied in two clinical trials, essentially curing 13 of 16 infants, restoring a normal immune system without the need for additional immune-related therapies. Approximately 3 years after their gene therapy, tragically, 3 of these children, all from the same trial, developed leukemia as a result of this experimental treatment. The current understanding of the mechanism behind this leukemogenesis involves three critical and cooperating factors,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Retrovirus; Insertional mutagenesis; Severe combined immune deficiency; Gene therapy; Adverse event; Clinical trial. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500002 Overexpression of kermit/dGIPC is associated with lethality in Drosophila melanogaster BJMBR Pereira,G.B.; Valente,V.; de Queiroz,M.S.; Vianna,M.C.B.; Paçó-Larson,M.L.. Insertional mutagenesis is an important tool for functional genomics in Drosophila melanogaster. The insertion site in the KG00562 mutant fly line has been mapped to the CG8709 (herein named DmLpin) locus and to the 3’ of kermit (also called dGIPC). This mutant line presents a high lethality rate resulting from a gain of function. To obtain some insight into the biological role of the mutated locus, we have characterized the mutation and its relation to the high mortality of the KG00562 fly line. In this mutant, we did not detect one of the DmLpin transcripts, namely DmLpinK, but we did detect an unusual 2.3-kb mRNA (LpinK-w). Further investigation revealed that the LpinK-w transcript results from an aberrant splicing between the untranslated first exon of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Insertional mutagenesis; Aberrant splicing; DmLpin; Kermit/dGIPC. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400003 Registros recuperados: 3 Primeira ... 1 ... Última

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