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| Gorgisen,Gokhan; Hapil,Fatma Zehra; Yilmaz,Ozlem; Cetin,Zafer; Pehlivanoglu,Suray; Ozbudak,Irem Hicran; Erdogan,Abdullah; Ozes,Osman Nidai. |
| Abstract Lung cancer is the leading cause of cancer-related death, and NSCLC constitutes nearly 85%–90% of all cases. The IRS proteins function as adaptors and transmit signals from multiple receptors. Upon binding of insulin to the insulin receptor (IR), IRS1 is phosphorylated at several YXXM motifs creating docking sites for the binding of PI3Kp85, which activates AKT kinase. Therefore, we thought that gain of function mutantions of IRS1 could be related to development of lung cancer. In line with this, we wanted determine whether the IRS1 gene was mutated in the coding regions surrounding YXXM motifs. We sequenced the coding regions surrounding YXXM motifs of IRS1 using tumor samples of 42 NSCLC patients and 40 matching controls and found heterozygote... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: IRS1; NSCLC; Insulin signaling; Lung cancer; IRS proteins. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100015 |
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| De Paula,Flávia MM; Boschero,Antonio C; Carneiro,Everardo M; Bosqueiro,José R; Rafacho,Alex. |
| Chronic administration of glucocorticoids induces insulin resistance that is compensated by an increase in p-cell function and mass. Since insulin signaling is involved in the control of p-cell function and mass, we investigated the content of insulin pathway proteins in pancreatic islets. Rats were made insulin resistant by daily administration of dexamethasone (1mg/kg, b.w., i.p.) for 5 consecutive days (DEX), whilst control rats received saline (CTL). Circulating insulin and insulin released from isolated islets were measured by radioimmunoassay whereas the content of proteins was analyzed by Western blotting. DEX rats were hyperinsulinemic and exhibited augmented insulin secretion in response to glucose (P < 0.01). The IRa-subunit, IRS-1, Shc, AKT,... |
| Tipo: Journal article |
Palavras-chave: Dexamethasone; Glucocorticoid; Insulin resistance; Insulin signaling; Pancreatic islets. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300006 |
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| Rafacho,A.; Giozzet,V.A.G.; Boschero,A.C.; Abrantes,J.L.F.; Cestari,T.M.; Carneiro,E.M.; Bosqueiro,J.R.. |
| A low-protein diet leads to functional and structural pancreatic islet alterations, including islet hypotrophy. Insulin-signaling pathways are involved in several adaptive responses by pancreatic islets. We determined the levels of some insulin-signaling proteins related to pancreatic islet function and growth in malnourished rats. Adult male Wistar rats (N = 20 per group) were fed a 17% protein (normal-protein diet; NP) or 6% protein (low-protein diet; LP), for 8 weeks. At the end of this period, blood glucose and serum insulin and albumin levels were measured. The morphometric parameters of the endocrine pancreas and the content of some proteins in islet lysates were determined. The β-cell mass was significantly reduced (≅65%) in normoglycemic but... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Β-cell mass; Insulin signaling; Low-protein diet; Pancreatic islets; Protein phosphorylation. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000010 |
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