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Comparison of traditional microscopy and digitized image analysis to identify and delineate pelagic fish egg spatial distribution ArchiMer
Lelievre, Stephanie; Antajan, Elvire; Vaz, Sandrine.
One of the problems concerning studies of fish egg distribution is the weak spatial and temporal resolution due to the workload that examination of a large number of samples would demand. Recently, the development of a new laboratory imaging system, the ZooScan, capable of obtaining relatively good resolution images enables automated zooplankton identification using supervised learning algorithms. This new approach was applied to formalin-fixed fish egg samples collected during French winter IBTS (International Bottom Trawl Surveys) in the Eastern English Channel and the Southern North Sea. Fish egg spatial distributions of seven species based on the microscope and ZooScan identifications were compared. Abundance and distribution maps of winter-spawning...
Tipo: Text Palavras-chave: Fish eggs; ZooScan; Image analysis; Supervised learning method; Interpolated map.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00074/18552/16103.pdf
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Identification and characterisation of winter spawning ground in the English Channel and Southern North Sea ArchiMer
Lelievre, Stephanie.
A better knowledge and monitoring of principal commercial fish spawning grounds have become necessary in the North Sea. The efficiency of CUFES was proved by sampling pelagic fish eggs in winter in Eastern Channel and Southern North Sea. Fish egg taxonomic identification based on visual criteria cannot always be carried out effectively. In particular, cod (Gadus morhua), and whiting (Merlangius merlangus) or flounder (Platichthys flesus) and dab (Limanda limanda) have the same range of egg diameter and similar morphologies. Alternative identification methods using molecular techniques were developed to improve the accuracy of egg taxonomic identification. First, PCR-RFLP method, then, in order to accelerate egg identification, the use of a new laboratory...
Tipo: Text Palavras-chave: Oeufs de poissons; CUFES; VET; PCR-RFLP; Cyt b; 16S; ZooScan; Analyse d’images; Analyses géostatistiques; Interpolation; Modélisation d’habitat; GLM; RQ; Fish eggs; CUFES; VET; Image analysis; ZooScan; PCR-RFLP; Geostatistical analyses; Interpolated map; Habitat modelling; GLM; RQ.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00043/15398/12757.pdf
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PCR-RFLP analyses of formalin-fixed fish eggs for the mapping of spawning areas in the Eastern Channel and Southern North Sea ArchiMer
Lelievre, Stephanie; Verrez-bagnis, Veronique; Jerome, Marc; Vaz, Sandrine.
A better knowledge on spawning grounds of principal commercial fishes from North Sea is necessary for their monitoring. Fish egg taxonomic identification based on morphological characters cannot always be carried out effectively. This is particularly the case for a number of Gadidae fish species eggs (i.e. cod, haddock and whiting, and three rockling species) which have the same range of size and for which molecular-based techniques can help improving the accuracy of egg taxonomic identification, and hence the reliability of studies on spawning areas. In this study, formalin fixation, egg conservation and DNA extraction were optimized in order to develop a specific PCR-RFLP method. Based on the sequence of the cytochrome b gene of reference specimens, a...
Tipo: Text Palavras-chave: Fish eggs; PCR-RFLP; Cytochrome b gene; Geostatistical analyses; Interpolated map.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00005/11613/8259.pdf
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