|
|
|
|
|
| SILVA,RAIMUNDO DA; SILVA,DANILLO; VENEZA,IVANA; SAMPAIO,IRACILDA; SCHNEIDER,HORACIO; GOMES,GRAZIELLE. |
| ABSTRACT In the present study, a novel set of eight EPIC primers were developed for Lutjanus purpureus and assayed in five other marine teleosts including three lutjanids, one scianid and one anablepid. Most of the genomic regions used in this study presented genetic diversity indexes equal or greater than the intragenic regions commonly used in population genetics studies. Moreover, six out of eight markers showed cross-amplification with other taxa. Thus, the primers described here may be used to elucidate questions at the intraspecific level for a large number of taxa. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: EPIC-PCR; Intron; Lutjanus; Southern Red Snapper. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502095 |
| |
|
| |
|
|
| Gerard, K.; Guilloton, E.; Arnaud-haond, Sophie; Aurelle, D.; Bastrop, R.; Chevaldonne, P.; Derycke, S.; Hanel, R.; Lapegue, Sylvie; Lejeusne, C.; Mousset, Sylvain; Ramsak, A.; Remerie, T.; Viard, Frederique; Feral, Jean-pierre; Chenuil, A.. |
| Exon Primed Intron Crossing (EPIC) markers providemolecular tools that are susceptible to be variable within specieswhile remaining amplifiable by PCR using potentially universal primers. In this studywe tested the possibility of obtaining PCR products from 50 EPIC markers on 23 species belonging to seven different phyla (Porifera, Cnidaria, Arthropoda, Nematoda, Mollusca, Annelida, Echinodermata) using 70 new primer pairs. A previous study had identified and tested those loci in a dozen species, including another phylum, Urochordata (Chenuil et al., 2010). Results were contrasted among species. The best results were achieved with the oyster (Mollusca) where 28 loci provided amplicons susceptible to contain an intron according to their size. This was... |
| Tipo: Text |
Palavras-chave: Universal primers; Alternative barcoding; Non-model species; Genetic marker; Intron. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00166/27775/25969.pdf |
| |
|
|
|
