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| GOLUNSKI,SIMONE; SILVA,MARCELI F.; MARQUES,CAMILA T.; ROSSETO,VANUSA; KAIZER,ROSILENE R.; MOSSI,ALTEMIR J.; RIGO,DIANE; DALLAGO,ROGÉRIO M.; DI LUCCIO,MARCO; TREICHEL,HELEN. |
| ABSTRACT The present study evaluated the purification of inulinase by changing the ionic strength of the medium by addition of NaCl and CaCl2 followed by precipitation with n-propyl alcohol or iso-propyl alcohol. The effects of the concentration of alcohols and the rate of addition of alcohols in the crude extract on the purification yield and purification factor were evaluated. Precipitation caused an activation of enzyme and allowed purification factors up to 2.4-fold for both alcohols. The purification factor was affected positively by the modification of the ionic strength of the medium to 0.5 mol.L-1 NaCl before precipitation with the alcohol (n-propyl or iso-propyl). A purification factor of 4.8-fold and an enzyme yield of 78.1 % could be achieved by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Inulinases; Ionic strength; Precipitation; Low cost.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000100057 |
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| Pintro,José Carlos; Taylor,Gregory James; Matumoto-Pintro,Paula Toshimi. |
| We conducted experiments designed to assess whether plants can be grown under conditions of low ionic strength simulating natural soil conditions. Wheat (Triticum aestivum, cv. Atlas 66) plants were grown using ten different relative nutrient addition rates (RAR) of nutrients (0.00, 0.06, 0.09, 0.12, 0.15, 0.18, 0.21, 0.24, 0.27, and 0.30 day-1) with a background solution containing 50 µmol.L-1 N and all other essential nutrients in optimal proportions relative to N. The relative growth rate (RGR) of plants over a 19-day experimental period responded directly to RAR (from 0.06 to 0.21 day-1) with a linear slope of 0.56 and an intercept of 0.085 day-1. Solution electrical conductivity (EC) values remained relatively stable over the experimental period. We... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Growth; Ionic strength; Nutrient solution; Wheat. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202004000300006 |
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| Ernani,Paulo Roberto; Barber,Stanley Arthur. |
| Concentration and identity of cations and anions in the soil solution may affect soil P reactions and thus P availability. The magnitude of these reactions was evaluated in this research after application of various salts to a highly weathered low pH soil. Chloride, nitrate, and sulfate salts of Na, NH4, K, Ca, Mg, Sr, or Cu were added to the soil after addition of 360mg P/kg trying to simulate ion concentrations around granules of fertilizers in the soil. Thirty days later, P was determined in the soil solution (Pli) and on the solid phase (Psi). The soil samples of some treatments were leached with water and three days later a new soil solution was displaced. Separate addition of all salts increased Pli, except NaCl at the lowest rate. The increase of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acid soils; Phosphorus reactions; Ionic strength. |
| Ano: 1995 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781995000200007 |
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| Ahmed,M; Latif,N; Khan,RA; Ahmad,A; Rocha,JBT; Mazzanti,CM; Bagatini,MD; Morsch,VM; Schetinger,MRC. |
| This study analyses venom from the elapid krait snake Bungarus sindanus, which contains a high level of acetylcholinesterase (AChE) activity. The enzyme showed optimum activity at alkaline pH (8.5) and 45ºC. Krait venom AChE was inhibited by substrate. Inhibition was significantly reduced by using a high ionic strength buffer; low ionic strength buffer (10 mM PO4 pH 7.5) inhibited the enzyme by 1. 5mM AcSCh, while high ionic strength buffer (62 mM PO4 pH 7.5) inhibited it by 1 mM AcSCh. Venom acetylcholinesterase was also found to be thermally stable at 45ºC; it only lost 5% of its activity after incubation at 45ºC for 40 minutes. The Michaelis-Menten constant (Km) for acetylthiocholine iodide hydrolysis was found to be 0.068 mM. Krait venom... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acetylcholinesterase; Inhibition; Krait; Ionic strength; Acetylthiocholine iodide; Bungarus sindanus; Snake venom. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200014 |
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| Casagrande,José Carlos; Soares,Marcio Roberto; Mouta,Ernesto Rinaldi. |
| The objective of this work was to assess the effects of pH and ionic strength upon zinc adsorption, in three highly weathered variable charge soils. Adsorption isotherms were elaborated from batch adsorption experiments, with increasing Zn concentrations (0-80 mg L-1), and adsorption envelopes were constructed through soil samples reactions with 0.01, 0.1 and 1 mol L-1 Ca(NO3)2 solutions containing 5 mg L-1 of Zn, with an increasing pH value from 3 to 8. Driving force of reaction was quantified by Gibbs free energy and separation factor. Isotherms were C-, H- and L-type and experimental results were fitted to nonlinear Langmuir model. Maximum adsorption ranged from 59-810 mg kg-1, and Zn affinity was greater in subsoil (0.13-0.81 L kg-1) than in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: PH; Adsorption envelope; Adsorption isotherm; Ionic strength; Trace element. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000100017 |
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