Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados Biol. Res. (2) Autor BALDEIRAS,INÊS E (1) MEARS,DAVID (1) ROJAS,EDUARDO (1) ROSÁRIO,LUÍS M (1) SANTOS,ROSA M (1) Palavra-chave Islet of Langerhans (2) 5-HT amperometry (1) BAPTA (1) Cytosolic free Ca2+ concentration (1) Inactivation kinetics (1) Insulin (1) Ion channel (1) Protein kinase C (1) Pulsatile insulin release (1) Stimulus-secretion coupling (1) Thymeleatoxin (1) Mais... Tipo do documento Journal article (2) Ano 2006 (2) País Chile (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Protein kinase C isoform specificity of cholinergic potentiation of glucose-induced pulsatile 5-HT/ insulin release from mouse pancreatic islets Biol. Res. BALDEIRAS,INÊS E; SANTOS,ROSA M; ROSÁRIO,LUÍS M. Thymeleatoxin (TMX), an activator of Ca2+-sensitive protein kinase C (cPKC) isoforms, was used to assess the PKC isoform specificity of cholinergic potentiation of glucose (11 mM)-induced pulsatile 5-HT/insulin release (PIR) from single mouse pancreatic islets. TMX (100 nM) and carbachol (Cch, 50 mM) enhanced PIR ~ 3-fold while reducing the underlying [Ca2+]i oscillations (duration and amplitude) by ~ 40-50%. Both effects were ablated by the specific PKC inhibitor bisindolylmaleimide and chronic TMX pretreatment. Cch also evoked an initial transient [Ca2+]i rise and surge of 5-HT release, which remained unaffected by chronic TMX pretreatment. It is concluded that the immediate cholinergic responses are insensitive to cPKC. In contrast, specific activation... Tipo: Journal article Palavras-chave: Cytosolic free Ca2+ concentration; 5-HT amperometry; Islet of Langerhans; Protein kinase C; Pulsatile insulin release; Thymeleatoxin. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300014 Properties of voltage-gated Ca2+ currents measured from mouse pancreatic β-cells in situ Biol. Res. MEARS,DAVID; ROJAS,EDUARDO. We used the single-microelectrode voltage-clamp technique to record ionic currents from pancreatic β-cells within intact mouse islets of Langerhans at 37C, the typical preparation for studies of glucose-induced "bursting" electrical activity. Cells were impaled with intracellular microelectrodes, and voltage pulses were applied in the presence of tetraethylammonium. Under these conditions, a voltage-dependent Ca2+ current (I Cav), containing L-type and non-L-type components, was observed. The current measured in situ was larger than that measured in single cells with whole-cell patch clamping, particularly at membrane potentials corresponding to the action potentials of β-cell electrical activity. The temperature dependence of I Cav was... Tipo: Journal article Palavras-chave: Islet of Langerhans; Insulin; Stimulus-secretion coupling; Ion channel; Inactivation kinetics; BAPTA. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300012 Registros recuperados: 2 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco