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| Labasque, Thierry; Chaumery, Christian; Aminot, Alain; Kergoat, Gilles. |
| The spectrophotometric iodine measurement for oxygen determination by the Winkler method is re-examined for theoretical and operational aspects. It is shown that the selection of an isobestic point for measuring the mixture of iodine and tri-iodide in the solution enhances reliability. The wavelength value of 466 nm was selected after a spectrum study. Then, performances are assessed by mean of statistical approach based on repeated standardization curves at the isobestic point compared with other wavelengths. At 466 nm, the method is linear up to about 1000 mumol kg(-1) of O-2. Optimization and validation of the spectrophotometric method is realized through a robustness study based on factors that may alter the results (temperature, reagent volumes,... |
| Tipo: Text |
Palavras-chave: Isobestic point; Sea water; Spectrophotometry; Dissolved oxygen. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-1694.pdf |
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