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Gene-centromere mapping in meiotic gynogenetic European seabass ArchiMer
Oral, Munevver; Colleter, Julie; Bekaert, Michhttps://w3.ifremer.fr/archimer-admin/author.jsp#ael; Taggart, John B.; Palaiokostas, Christos; Mcandrew, Brendan J.; Vandeputte, Marc; Chatain, Beatrice; Kuhl, Heiner; Reinhardt, Richard; Peruzzi, Stefano; Penman, David J..
Background Fully isogenic lines in fish can be developed using “mitotic” gynogenesis (suppression of first zygotic mitosis following inactivation of the sperm genome). However, genome-wide verification of the steps in this process has seldom been applied. We used ddRADseq to generate SNP markers in a meiotic gynogenetic family of European seabass (Dicentrarchus labrax): (i) to verify the lack of paternal contribution in a meiotic gynogenetic family; (ii) to generate a gene-centromere map from this family; (iii) to identify telomeric markers that could distinguish mitotic gynogenetics from meiotic gynogenetics, which sometimes arise spontaneously in mitotic gynogenetic families. Results From a single meiotic gynogenetic family consisting of 79 progeny, 42...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Meiotic gynogenesis; Isogenic lines; DdRAD seq; Genetic map; Gene-Centromere map; Aquaculture.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00388/49901/50462.pdf
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Assessment of Genetic Variability of Fish Personality Traits using Rainbow Trout Isogenic Lines ArchiMer
Millot, Sandie; Pean, Samuel; Labbe, Laurent; Kerneis, Thierry; Quillet, Edwige; Dupont-nivet, Mathilde; Begout, Marie-laure.
The study of inter-individual variability of personality in fish is a growing field of interest but the genetic basis of this complex trait is still poorly investigated due to the difficulty in controlling fish genetic origin and life history. When available, isogenic lines that allow performing independent tests on different individuals having identical genotype constitute a very relevant experimental material to disentangle the genetic and environmental components of behavioural individuality. We took advantage of heterozygous isogenic lines to investigate the personality in rainbow trout through the analysis of their reactions to different experimental situations. To this end, seven to ten rainbow trout isogenic lines were screened for their spatial...
Tipo: Text Palavras-chave: Personality; Isogenic lines; Genetic variability; Risk taking; Spatial exploration; Oncorhynchus mykiss.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00194/30496/29976.pdf
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