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	Provedor de dados BJM (1) Electron. J. Biotechnol. (1) Autor Manrich,Eiric (2) Fleuri,Luciana Francisco (1) Harumi Sato,Hélia (1) Kawaguti,Haroldo Yukio (1) Sato,Hélia Harumi (1) Yukio Kawaguti,Haroldo (1) Mais... Palavra-chave Glucosyltransferase (2) Isomaltulose (2) Batch process (1) Erwinia sp. (1) Free cells (1) Optimization (1) Response surface (1) Response surface methodology (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2006 (1) 2005 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Application of response surface methodology for glucosyltransferase production and conversion of sucrose into isomaltulose using free Erwinia sp. cells Electron. J. Biotechnol. Yukio Kawaguti,Haroldo; Manrich,Eiric; Harumi Sato,Hélia. Isomaltulose is a structural isomer of sucrose commercially used in food industries. Glucosyltransferase produced by Erwinia sp. D12 catalyses an intramolecular transglucosylation of sucrose giving isomaltulose. The Experimental design and response surface methodology were applied for the optimization of the nutrient concentration in the culture medium for the enzyme production in shaken flasks at 200 rpm and 30ºC. The three variables involved in this study were sugar cane molasses, bacteriological peptone and yeast extract Prodex Lac SD®. The statistical analysis of the results showed that, in the range studied, all the factors had a significant effect (p < 0.05) on glucosyltransferase production and the highest enzyme activity was observed in culture... Tipo: Journal article Palavras-chave: Batch process; Erwinia sp.; Free cells; Glucosyltransferase; Isomaltulose; Response surface methodology. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500003 Production of glucosyltransferase by Erwinia sp. using experimental design and response surface methodology BJM Kawaguti,Haroldo Yukio; Manrich,Eiric; Fleuri,Luciana Francisco; Sato,Hélia Harumi. Glucosyltransferase produced by strain Erwinia sp. is an intracellular enzyme that catalyzes the formation of isomaltulose from sucrose. Isomaltulose is a non-cariogenic reducing dissacharide commercially used in foods. Response surface methodology and 2³-factorial central composite design were employed to optimize a fermentation medium for the production of glucosyltransferase by Erwinia sp. in shaken flasks at 200 rpm and 30ºC. The three variables involved in this study were sugar cane molasses (SCM), corn steep liquor (CSL) and yeast extract Prodex Lac SD (YEP). The statistical analysis of the results showed that, in the range studied, all the factors had a significant effect on glucosyltransferase production and the optimum medium composition for... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucosyltransferase; Isomaltulose; Optimization; Response surface. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300005 Registros recuperados: 2 Primeira ... 1 ... Última

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