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	Provedor de dados Ciênc. Tecnol. Aliment. (2) BJM (1) Electron. J. Biotechnol. (1) Autor Sato,Hélia Harumi (3) Kawaguti,Haroldo Yukio (2) Manrich,Eiric (2) Fleuri,Luciana Francisco (1) Harumi Sato,Hélia (1) Moraes,Ana Lúcia Leite (1) Pinheiro,Andrelina (1) Steckelberg,Claúdia (1) Yukio Kawaguti,Haroldo (1) Mais... Palavra-chave Isomaltulose (4) Erwinia sp. (2) Glucosyltransferase (2) Batch process (1) Calcium alginate (1) Erwinia sp D12 (1) Free cells (1) Glicosiltransferase (1) Immobilized cells (1) Imobilização celular (1) Optimization (1) Response surface (1) Response surface methodology (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Journal article (1) Ano 2011 (1) 2006 (1) 2005 (2) País Brazil (3) Chile (1) Idioma Inglês (3) Português (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Production of glucosyltransferase by Erwinia sp. using experimental design and response surface methodology BJM Kawaguti,Haroldo Yukio; Manrich,Eiric; Fleuri,Luciana Francisco; Sato,Hélia Harumi. Glucosyltransferase produced by strain Erwinia sp. is an intracellular enzyme that catalyzes the formation of isomaltulose from sucrose. Isomaltulose is a non-cariogenic reducing dissacharide commercially used in foods. Response surface methodology and 2³-factorial central composite design were employed to optimize a fermentation medium for the production of glucosyltransferase by Erwinia sp. in shaken flasks at 200 rpm and 30ºC. The three variables involved in this study were sugar cane molasses (SCM), corn steep liquor (CSL) and yeast extract Prodex Lac SD (YEP). The statistical analysis of the results showed that, in the range studied, all the factors had a significant effect on glucosyltransferase production and the optimum medium composition for... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucosyltransferase; Isomaltulose; Optimization; Response surface. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300005 Production of isomaltulose obtained by Erwinia sp. cells submitted to different treatments and immobilized in calcium alginate Ciênc. Tecnol. Aliment. Kawaguti,Haroldo Yukio; Sato,Hélia Harumi. In recent decades, there has been an increase in the studies of isomaltulose obtainment, due to its physicochemical properties and physiological health benefits. These properties, which include low cariogenicity, low glycemic index and greater stability, allow the use of this sweetener as a substitute for sucrose in foods; besides the fact that it can be converted to isomalt, a dietary non-cariogenic sugar alcohol used in pharmaceuticals as well as in the food industry. Isomaltulose (6-O-α-D-glucopyronosyl-1-6-D-fructofuranose) is a disaccharide reducer obtained by the enzymatic conversion of sucrose - the α-glucosyltransferase enzyme. Different treatments were performed for the preparation of whole cells; lysed cells; and crude enzyme extract of Erwinia... Tipo: Info:eu-repo/semantics/article Palavras-chave: Calcium alginate; Immobilized cells; Erwinia sp.; Isomaltulose. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000100039 Produção de isomaltulose a partir da transformação enzimática da sacarose, utilizando-se Erwinia sp D12 imobilizada com alginato de cálcio Ciênc. Tecnol. Aliment. Moraes,Ana Lúcia Leite; Steckelberg,Claúdia; Sato,Hélia Harumi; Pinheiro,Andrelina. A glicosiltransferase de Erwinia sp D12 é capaz de converter a sacarose em isomaltulose (6-o-alfa-glicopiranosil D-frutofuranose), um açúcar alternativo que apresenta baixo potencial cariogênico, e que pode ser utilizado em chocolates, gomas de mascar e balas. A isomaltulose é também utilizada na produção de isomalte, uma mistura de açúcar álcool, de baixo valor calórico e baixo potencial cariogênico. No estudo da influência dos componentes do meio de cultivo, na produção de glicosiltransferase, em frascos agitados, foi obtido maior atividade da enzima (12,8 unidades de atividade/mL do meio de cultura) em meio de cultura A constituído de melaço 12% (p/v) de sólidos solúveis totais, peptona 4% (p/v) e extrato de carne 0,4% (p/v). No estudo do efeito do... Tipo: Info:eu-repo/semantics/article Palavras-chave: Isomaltulose; Glicosiltransferase; Erwinia sp D12; Imobilização celular. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612005000100016 Application of response surface methodology for glucosyltransferase production and conversion of sucrose into isomaltulose using free Erwinia sp. cells Electron. J. Biotechnol. Yukio Kawaguti,Haroldo; Manrich,Eiric; Harumi Sato,Hélia. Isomaltulose is a structural isomer of sucrose commercially used in food industries. Glucosyltransferase produced by Erwinia sp. D12 catalyses an intramolecular transglucosylation of sucrose giving isomaltulose. The Experimental design and response surface methodology were applied for the optimization of the nutrient concentration in the culture medium for the enzyme production in shaken flasks at 200 rpm and 30ºC. The three variables involved in this study were sugar cane molasses, bacteriological peptone and yeast extract Prodex Lac SD®. The statistical analysis of the results showed that, in the range studied, all the factors had a significant effect (p < 0.05) on glucosyltransferase production and the highest enzyme activity was observed in culture... Tipo: Journal article Palavras-chave: Batch process; Erwinia sp.; Free cells; Glucosyltransferase; Isomaltulose; Response surface methodology. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500003 Registros recuperados: 4 Primeira ... 1 ... Última

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