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Karyotype and genome size analyses in species of Helichrysum (Asteraceae) Acta Botanica
Azizi,Narjes; Sheidai,Masoud; Mozaffarian,Valiollah; Nourmohammadi,Zahra.
Karyotype studies were performed in 18 populations of eight Helichrysum species in Iran. Those species showed chromosome numbers of 2n = 2x = 14; 2n = 4x = 24, 28 and 32; 2n = 6x = 36; 2n = 7x = 42; 2n = 8x = 48; 2n = 9x = 54; and 2n = 10x = 60. The chromosome numbers of H. davisianum, H. globiferum, H. leucocephalum and H. oocephalum are reported here for the first time. New ploidy levels are reported for H. oligocephalum (2n = 4x = 24) and H. plicatum (2n = 4x = 32). The chromosomes were metacentric and submetacentric. An ANOVA among H. globiferum and H. leucocephalum populations showed significant differences for the coefficient of variation for chromosome size, total form percentage and the asymmetry indices, indicating that changes in the chromosome...
Tipo: Info:eu-repo/semantics/article Palavras-chave: C-value; Helichrysum; Karyotype; Polyploidy level.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000300009
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Updating the list of chromosome numbers for Philodendron (Araceae) Acta Botanica
Vasconcelos,Emanuelle V.; Brasileiro-Vidal,Ana C.; Benko-Iseppon,Ana M.; Vasconcelos,Santelmo.
ABSTRACT Aiming for a better understanding of karyotype evolution within Philodendron, we report chromosome counts for 23 species of the genus, of which 19 are being reported for the first time, thus increasing to 84 ( ca. 17 % of the genus) the total number of species with available chromosome counts. The diploid numbers 2 n = 32 and 2 n = 34 were the most common, with 10 and 11 species, respectively, whereas only two species presented different chromosome numbers ( P. giganteum with 2 n = 30 and P. adamantinum with 2 n = 36). The results are discussed in the context of previous analyses of karyotypes of Philodendron spp., taking into account bidirectional dysploidy as the main mechanism of chromosome number evolution within the genus.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aroids; Diploid number; Dysploidy; Karyotype; Philodendron.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000200309
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Karyotypic analysis in species of the genus Dasyprocta (Rodentia: Dasyproctidae) found in Brazilian Amazon Anais da ABC (AABC)
RAMOS,ROSEMAR S. L.; VALE,WILLIAM G.; ASSIS,FÁTIMA L..
A total of 30 animals of the genus Dasyprocta were cytogenetically studied. They belong to the following species: D. prymnolopha (N=20), D. leporina (N=6), D. fuliginosa (N=1) and Dasyprocta sp. (N=3) (Dasyproctidae, Hystricognathi). Cell suspensions were obtained by peripheral blood culture, besides bone marrow and spleen cells, from D. prymnolopha and D. leporina. The diploid number was 64/65 for all samples. The karyotypes showed similarity, and chromosomal polymorphism was not detected by Giemsa conventional staining and G banding. The constitutive heterochromatin distribution at the pericentromeric region of all the chromosomes was similar in all species. D. prymnolopha, D. leporina and Dasyprocta sp. presented variation in the heterochromatical block...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytogenetics; Hystricognathi; Dasyprocta; Karyotype.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652003000100007
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Range distribution and contributions to taxonomy of Elops smithi (ELOPIFORMES: ELOPIDAE) Anais da ABC (AABC)
SOUSA,RODRIGO P.C. DE; SODRÉ,DENISE; COSTA,RAUQUÍRIO M. DA; VALLINOTO,MARCELO; OLIVEIRA,EDIVALDO H.C.; SILVA-OLIVEIRA,GLÁUCIA C.; SAMPAIO,IRACILDA; GUIMARÃES-COSTA,AURYCÉIA.
Abstract: This study presents the first record of Elops smithi for northern Brazil. The evidence suggests this species is being misidentified incorrectly as Elops saurus in estuaries of the Western Atlantic Ocean. Here, morphological, molecular, and cytogenetic evidence identified all ladyfish specimens from one estuary in the region as E. smithi. Thus, at least Elops smithi occurs in the northern coast of Brazil and it is recommended that specimens from this region identified as E. saurus be further investigated with genetic and cytogenetic tools in order to assure a correct species identification.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Elopidae; Karyotype; Ladyfish; Malacho; Mitochondrial DNA.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700622
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Cytogenetics and Molecular Genetic Analysis of Chimerism in Marmosets (Callithrix: Primates) Anais da ABC (AABC)
SILVA,MONIQUE O.M.; ARMADA,JORGE LUÍS A. DE; VERONA,CARLOS EDUARDO S.; HELIODORO,GABRIELA; NOGUEIRA,DENISE M..
ABSTRACT The birth of fraternal twins is a characteristic frequently observed in callitrichids. Cytogenetic studies have demonstrated hematopoietic chimerism in marmosets with the occurrence of two cell lines 2n=46,XX/46,XY in females and males co-twins, without phenotypic changes. Amplification by PCR have also been used to verify the presence of the SRY gene in female chimaeras. Our aim was to verify the occurrence of chimerism in Callithrix sp. individuals considered as hybrids according to their intermediate phenotypes between C. jacchus and C. penicillata. Blood samples from 37 Callithrix sp. individuals were collected. Hematopoietic chimerism 2n=46,XX/46,XY was detected by cytogenetic analysis in five individuals, three males and two females. A...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Karyotype; SRY; Chimaeras; Hybridization.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000602793
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Karyological and electrophoretic differences between Pomacea flagellata and P. patula catemacensis: Caenogastropoda: Ampullariidae Biocell
Diupotex-Chong,María Esther; Cazzaniga,Néstor J; Uribe-Alcocer,Manuel.
The widespread Mexican apple snail Pomacea flagellata (Say 1827) and the strictly endemic "tegogolo" P. patula catemacensis (Baker 1922) (restricted to Lake Catemaco), are the only known American Ampullariidae that have haploid complements n=13. Pomacea patula catemacensis has suffered a critical reduction in abundance due to immoderate fishing for human consumption. Chromosome slides were obtained from colchicine-injected Pomacea snails collected from nine locations along the coastal zone of the Gulf of Mexico, including Lake Catemaco, for use in principal component analysis (PCA). Total proteins in foot homogenates were analyzed through isoelectric focusing (IEF) and native-PAGE electrophoresis on polyacrylamide gels. The chromosome number 2n=26 was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apple snails; Endangered species; Isoelectric focusing; Karyotype; Mexico; Native-PAGE electrophoresis.
Ano: 2007 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300002
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Multicolor fluorescence in situ hybridization with combinatorial labeling probes enables a detailed karyotype analysis of Larix principis-rupprechtii Biol. Res.
LIU,BO; LIWANG,QI; RUIYANG,CHEN; SONG,WENQIN.
The chromosomes (2n = 2x = 24) of Larix principis-rupprechtii are composed of six pairs of large metacentrics and six pairs of medium-sized submetacentrics. The identification of homologous pairs is hampered by their high degree of similarity at the morphological level in each group. As one of the most extensively used methods in molecular cytogenetics producing chromosome landmarks, fluorescence in situ hybridization (FISH) has significantly facilitated karyotype construction, especially in species with morphologically similar chromosomes. This study developed a simple but effective use of combinatorial labeling probes to distinguish chromosomes of Larix principis-rupprechtii by multicolor FISH. Three highly repetitive sequences in Larix were selected:...
Tipo: Journal article Palavras-chave: Combinatorial labeling probes; Fluorescence in situ hybridization; Karyotype; Larix principis-rupprechtii; LPD repeats; 25S and 5S rDNA.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100003
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KARYOLOGY OF MUGIL LIZA AND M. CUREMA FROM VENEZUELA Boletín de Investigaciones
Nirchio T.,Mauro; Cequea,Hernán.
Karyotypes of Mugil liza and M. curema from Venezuela were studied. M. liza karyotype is 2n=48 acrocentric chromosomes, but M. curema is 2n=24, containing one submetacentric and 11 metacentric pairs. The chromosomic complement described for M. liza constitutes the first report for the species and coincides with the modal karyotype (2n=48) in the Mugilidae family. Venezuelan M. curema karyotype differs in number and shape from M. curema from Louisiana (USA), which has been reported to have a complement 2n=28 (10 metacentric pairs, two subtelocentric pairs and two acrocentric pairs).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Karyotype; Chromosomes.
Ano: 1998 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97611998000100004
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Karyotype analysis of Minuartia mesogitana subsp. mesogitana and Minuartia elmalia (Caryophyllaceae, Alsinoideae) Boletín de la Sociedad
Yüce,Oguz; Eroglu,Halil Erhan; Koç,Murat; Hamzaoglu,Ergin.
Mitotic metaphase chromosomes, karyotypic characters, monoploid karyograms and ideograms of Minuartia mesogitana subsp. mesogitana and Minuartia elmalia were investigated. Analysis of somatic metaphases showed that the chromosome numbers and the karyotype formula of these taxa were 2n = 2x = 24 = 8m + 8sm + 8st for Minuartia mesogitana subsp. mesogitana and 2n = 2x = 32 = 12m + 16sm + 4st for Minuartia elmalia. No satellites were observed in the karyotypes of the taxa. The intrachromosomal and interchromosomal karyotype asymmetries were estimated with Mean Centromeric Asymmetry (MCA) and Coefficient of Variation of Chromosome Length (CVCL).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Minuartia; Karyotype; Ideogram; Karyotype asymmetry.
Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722016000300006
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Karyotypic studies and morphological analysis of some reproductive features in five species of Conyza (Astereae: Asteraceae) from Northeastern Argentina Boletín de la Sociedad
Urdampilleta,Juan D.; Amat,Aníbal G.; Bidau,Claudio J..
The cosmopolitan genus Conyza Less. comprises about 100 species, 22 of which occur in Argentina. Current taxonomic treatments, largely based on exomorphological characters, are insufficient to characterize and circumscribe some of their polymorphic species. Interspecific variations in inflorescences typology and capitula structure, as well as karyotypic aspects, were studied in five species of Conyza that naturally occur in Misiones Province (Argentina): C. blakei (Cabrera) Cabrera, C. bonariensis (L.) Cronquist var. bonariensis, C. glandulitecta Cabrera, C. primulaefolia (Lam.) Cuatrec. & Lourteig, C. sumatrensis (Retz.) E. Walker var. sumatrensis and C. sumatrensis (Retz.) E. Walker var. floribunda (Kunth) J. B. Marshall. Chromosome numbers for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Asteraceae; Astereae; Chromosomes; Conyza; Inflorescence; Interspecific variation; Karyotype.
Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722005000100010
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Karyotipic asymmetry of both wild and cultivated species of Pennisetum Bragantia
Techio,Vânia Helena; Davide,Lisete Chamma; Cagliari,Alexandro; Barbosa,Sandro; Pereira,Antônio Vander.
This study aimed the establishment of the relation between karyotipic asymmetry values obtained for different accessions of both wild and cultivated species of Pennisetum from Germplasm Bank of Embrapa Gado de Leite/Juiz de Fora-Minas Gerais State, Brazil. Conventional cell cycle synchronization protocols and Feulgen staining method were used to obtain metaphases plates. The wild-type accessions corresponded to the species P. setosum (2n=6x=54), P. nervosum (2n=4x=36), and P. orientale (2n=4x=36), and the cultivated to P. purpureum (2n=4x=28) and P. glaucum (2n=2x=14). No significant difference was found for the total length of chromosomes (p>0.05) among the species. The analysis of intra-chromosomal asymmetry (A1) and inter-chromosomal asymmetry (A2)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pennisetum purpureum; Pennisetum glaucum; Germplasm; Karyotype.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052010000200003
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Cytomolecular characterization of cultivars and landraces of wheat tolerant and sensitive to aluminum toxicity Bragantia
Oliveira,Érica Cristina de; Pinto-Maglio,Cecília Alzira Ferreira.
ABSTRACT Karyotyping and chromosome complement knowledge are a prerequisite for cytogenetic mapping. The aim of this study was to characterize 1 common wheat cultivar (BH 1146) and 4 durum wheat genotypes (IAC 1003, Langdon, P19 and P33) from the breeding program of the Agronomic Institute of Campinas (IAC), using karyotype analysis together with Chromomycin A3/4’-6-Diamidino-2-phenylindole (CMA/DAPI) banding techniques for longitudinal characterization of chromosomes and fluorescence in situ hybridization (FISH) in the mapping of the 45S rDNA region. Durum wheat chromosome complement number was 2n = 4x = 28 with the following karyotype formula: 22m + 6sm. Common wheat chromosome complement number was 2n = 6x = 42 with the following karyotype formula: 34m...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Triticum turgidum ssp. durum; Karyotype; CMA/DAPI banding; FISH-rDNA; Cytomolecular mapping.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052017000400456
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Cytogenetic studies in some species of Passiflora L. (Passifloraceae): a review emphasizing Brazilian species BABT
Souza,Margarete Magalhães; Pereira,Telma N. Santana; Vieira,Maria Lúcia Carneiro.
The Passifloraceae is represented by species of tropical and subtropical origin. The Passiflora is the richest genus with approximately 450 species, 200 of them being native to Brazil. Recent karyological studies have reported the basic chromosome number for the Passiflora genus as x = 6, whereas x = 9, x = 10 and x = 12 were established as secondary basic numbers. High rates of fertility occur in most Passiflora species, since both meiotic index and pollen viability are above 90%. Unusual meiotic behavior has been described in some taxa. Unviable pollen were observed in some diploids species. The genome size varies from 1.83 to 5.36 pg, and significant interspecific variance has been observed. Studies using the FISH methodology have shown that there are...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Karyotype; Banding; Meiotic behavior; Pollen viability; Nuclear DNA content; FISH.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200003
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Chromosomal studies of five species of the marine fishes from the Paranaguá Bay and the karyotypic diversity in the marine teleostei of the Brazilian coast BABT
Cipriano,Roger Raupp; Fenocchio,Alberto Sérgio; Artoni,Roberto Ferreira; Molina,Wagner; Noleto,Rafael Bueno; Kantek,Daniel Luis Zanella; Cestari,Marta Margarete.
In this study, five species of marine fishes from the Paranaguá Bay in the Brazilian coast were evaluated. Eucinostomus argenteus and Diapterus rhombeus (Gerreidae) presented 48 chromosomes, all of which more acrocentric (FN = 48); Strongylura timucu and S. marina (Belonidae) also presented 48 chromosomes, but with a higher karyotypic complexity than the Gerreidae, 10M+2SM+36A (FN = 60) and 4M+44A (FN = 52), respectively. The fifth species, Mugil curema (Mugilidae), different than the others, presented only 28 chromosomes 20M+4ST+4A (FN = 48). The species presented diversity in the karyotypic macro-structure, which should be relevant for the cytotaxonomy and the evolution of this group of the vertebrate.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Marine fishes; Karyotype; Cytotaxonomy; Karyotypic evolution.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200010
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Unexpected chromosomal alterations in Tayassu tajacu (Artiodactyla: Tayassuidae) in captivity Braz. J. Vet. Res. Anim. Sci.
Lima,José Fernando de Sousa; Guedes,Fátima Becker; Silva,Roxane Wirschum; Hass,Iris; Cavalli,Iglenir João; Silva,Juliana da; Freitas,Thales Renato de; Sbalqueiro,Ives José.
Wild animals have been used as bioindicators in situations in which the environment was exposed to chemical agents. In general, chemical agents may induce chromosomal aberrations, such as breaks and gaps. The peccary, Tayassu tajacu is a pig relative that exhibits a very stable karyotype with the only described alterations being of the form of the X chromosome. Chromosomal gaps and breaks were observed at high frequencies during cytogenetics analyses. These alterations were observed in the chromosomes autossomics. Reviews of the literature and of the data described herein suggests that an vermifuge, the ivermectin base, was the most likely cause of these chromosomal alterations.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tayassu; Karyotype; Chromosome; Chromosomal alterations.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962004000100002
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Karyotype of a termitophilic species of Diplopoda (Polydesmida, Chelodesmidae) Braz. J. Genet.
Fontanetti,Carmem Silvia.
This report describes the karyotype of a Brazilian Diplopod species (Arthropoda), Sandalodesmus gasparae (Schubart, Acta Zool. Lilloana Inst. "Miguel Lillo" 2: 321-440,1944), collected from a termite mound Cornitermes bequaerti. This species has 2n = 12. The sex pair could not be distinguished.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Karyotype; Termitophilic; Diplopoda.
Ano: 1996 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400010
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Cytogenetic studies of Brazilian pediatric myelodysplastic syndrome cases: challenges and difficulties in a large and emerging country BJMBR
Velloso,E.D.R.P.; Chauffaille,M.L.; Peliçario,L.M.; Tanizawa,R.S.S.; Toledo,S.R.C.; Gaiolla,R.D.; Lopes,L.F..
Myelodysplastic syndromes (MDS) and juvenile myelomonocytic leukemia (JMML) are rare hematopoietic stem cell diseases affecting children. Cytogenetics plays an important role in the diagnosis of these diseases. We report here the experience of the Cytogenetic Subcommittee of the Brazilian Cooperative Group on Pediatric Myelodysplastic Syndromes (BCG-MDS-PED). We analyzed 168 cytogenetic studies performed in 23 different cytogenetic centers; 84 of these studies were performed in patients with confirmed MDS (primary MDS, secondary MDS, JMML, and acute myeloid leukemia/MDS+Down syndrome). Clonal abnormalities were found in 36.9% of the MDS cases and cytogenetic studies were important for the detection of constitutional diseases and for differential diagnosis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Childhood myelodysplastic syndromes; Juvenile myelomonocytic leukemia; Cytogenetics; Karyotype; Diagnosis.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100085
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Acute promyelocytic leukemia: the study of t(15;17) translocation by fluorescent in situ hybridization, reverse transcriptase-polymerase chain reaction and cytogenetic techniques BJMBR
Chauffaille,M.L.L.F.; Figueiredo,M.S.; Beltrani,R.; Antunes,S.V.; Yamamoto,M.; Kerbauy,J..
Acute promyelocytic leukemia (AML M3) is a well-defined subtype of leukemia with specific and peculiar characteristics. Immediate identification of t(15;17) or the PML/RARA gene rearrangement is fundamental for treatment. The objective of the present study was to compare fluorescent in situ hybridization (FISH), reverse transcriptase-polymerase chain reaction (RT-PCR) and karyotyping in 18 samples (12 at diagnosis and 6 after treatment) from 13 AML M3 patients. Bone marrow samples were submitted to karyotype G-banding, FISH and RT-PCR. At diagnosis, cytogenetics was successful in 10 of 12 samples, 8 with t(15;17) and 2 without. FISH was positive in 11/12 cases (one had no cells for analysis) and positivity varied from 25 to 93% (mean: 56%). RT-PCR was done...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acute promyelocytic leukemia; Karyotype; FISH; RT-PCR; PML/RARA genem rearrangement.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600006
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Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples BJMBR
Santos,M.F.M.; Oliveira,F.C.A.C.; Kishimoto,R.K.; Borri,D.; Santos,F.P.S.; Campregher,P.V.; Silveira,P.A.A.; Hamerschlak,N.; Mangueira,C.L.P.; Duarte,F.B.; Crepaldi,A.H.; Salvino,M.A.; Velloso,E.D.R.P..
Cytogenetics is essential in myeloid neoplasms (MN) and pre-analytical variables are important for karyotyping. We assessed the relationship between pre-analytical variables (time from collection to sample processing, material type, sample cellularity, and diagnosis) and failures of karyotyping. Bone marrow (BM, n=352) and peripheral blood (PB, n=69) samples were analyzed from acute myeloid leukemia (n=113), myelodysplastic syndromes (n=73), myelodysplastic syndromes/myeloproliferative neoplasms (n=17), myeloproliferative neoplasms (n=137), and other with conclusive diagnosis (n=6), and reactive disorders/no conclusive diagnosis (n=75). The rate of unsuccessful karyotyping was 18.5% and was associated with the use of PB and a low number of nucleated cells...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytogenetics; Karyotype; Leukemia; Myelodysplasia; Myeloproliferative disease.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200608
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Karyotype of cryopreserved bone marrow cells BJMBR
Chauffaille,M.L.L.F.; Pinheiro,R.F.; Stefano,J.T.; Kerbauy,J..
The analysis of chromosomal abnormalities is important for the study of hematological neoplastic disorders since it facilitates classification of the disease. The ability to perform chromosome analysis of cryopreserved malignant marrow or peripheral blast cells is important for retrospective studies. In the present study, we compared the karyotype of fresh bone marrow cells (20 metaphases) to that of cells stored with a simplified cryopreservation method, evaluated the effect of the use of granulocyte-macrophage colony-stimulating factor (GM-CSF) as an in vitro mitotic index stimulator, and compared the cell viability and chromosome morphology of fresh and cryopreserved cells whenever possible (sufficient metaphases for analysis). Twenty-five bone marrow...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Cryopreservation; Karyotype; Bone marrow cells.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700004
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