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Clinical and microbiological characterization of KPC-producing Klebsiella pneumoniae infections in Brazil BJID
Beirão,Elisa Maria; Furtado,Juvencio Jose Duailibe; Girardello,Raquel; Ferreira Filho,Heli; Gales,Ana Cristina.
In 2008 isolates of KPC-producing Klebsiella pneumoniae (KPC-KPN) were detected for the first time at Hospital Heliópolis, São Paulo, Brazil. The aim of this study was to characterize the clinical and microbiological outcomes of infections caused by KPC-KPN. A historical cohort of patients from whom KPC-KPN strains were isolated was performed. Isolates were identified as resistant to ertapenem by automated broth microdilution system and screened as carbapenemase producers by the modified Hodge test. The beta-lactamase resistance gene blaKPC was detected by PCR. The genetic relatedness of isolates was determined by PFGE. The study provides early clinical experience in treating KPC-KPN infections in a Brazilian tertiary center.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella pneumonia; KPC; Hospital infection; Carbapenemase.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000100013
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Characterization of Klebsiella pneumoniae associated with cattle infections in southwest China using multi-locus sequence typing (MLST), antibiotic resistance and virulence-associated gene profile analysis BJM
Cheng,Fangjun; Li,Zhangcheng; Lan,Shimei; Liu,Wei; Li,Xiaoyan; Zhou,Zuoyong; Song,Zhenhui; Wu,Juan; Zhang,Manli; Shan,Wenjie.
Abstract Klebsiella pneumoniae is important human and animal pathogen that causes a wide spectrum of infections. In this study, isolates from cattle nasal swabs samples were identified by 16S rRNA, and to evaluate the antimicrobial susceptibility, virulence gene carrying levels, and multilocus sequence typing of K. pneumoniae isolates. 33 isolates of K. pneumoniae were isolated and identified in 213 nasal swabs samples, of which 12 were hypervirulent K. pneumoniae strains. Extended Spectrum Beta-Lactamases genes were found in 93.4% of the strains. Of which, TEM was the most prevalent (93.4%), followed by CTX-M and SHV were 57.6% and 39.4%, respectively. A main mutation pattern of quinoloneresistance-determining region, Thr83-Ieu and Asp87-Asn in gyrA and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle; Antimicrobial susceptibility; Klebsiella pneumonia; MLST; Virulence genes.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500093
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