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Registros recuperados: 27
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The dispersal of nitrogen-fixing Enterobacteriaceae from sewage into the waters and sediments of Morecambe Bay, UK. ArchiMer
Jones, K; Betaieb, M.
The sediments of Morecambe Bay contain large numbers of salinity-tolerant, nitrogen-fixing Enterobacteriaceae (NFEs) mainly Klebsiella pneumoniae ) which appear to be derived from sewage. Sewage effluents, regardless as to whether they have received primary or secondary treatment, contain huge numbers of NFEs with salinity-tolerant and freshwater strains present in roughly equal quantities. The bacteria are discharged into the rivers and estuaries and carried out to sea. Once in the sea the salinity-tolerant strains retain their viability better than the freshwater strains so that the incoming tide, which doses the sediments, contains mainly salinity-tolerant NFEs. It is probable that similar processes occur all round the U.K. coast, as inshore coastal...
Tipo: Text Palavras-chave: Klebsiella pneumoniae; Enterobacteriaceae; Salinity tolerance; Microbial contamination; Pathogenic bacteria; Bacteria; Sewage; Nitrogen fixation; Sediment pollution; Sea water.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1003.pdf
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Assessment of genetic relationship between Klebsiella pneumoniae and Klebsiella oxytoca samples isolated from a dental office J. Venom. Anim. Toxins incl. Trop. Dis.
Pimenta-Rodrigues,MV; Fusco-Almeida,AM; Bertoni,BW; Pietro,RCLR.
The present study aimed to analyze the genetic similarity between genomic profiles of thirteen Klebsiella oxytoca and seven Klebsiella pneumoniae samples isolated from two different collections carried out in different places of dental offices. Random amplified polymorphic DNA (RAPD) technique and similarity coefficients (calculated by Sorensen-Dice and simple matching) were applied to determine their genetic profile of randomic DNA sequences. The majority of the isolates of K. pneumoniae and K. oxytoca presented similar coefficient values (e" 0.80). Thus, it was possible to identify that strain dissemination occurred mainly via the hands of the surgeon-dentists and, finally, to determine the genetic similarity of the strains from dental office environments.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella oxytoca; Klebsiella pneumoniae; RAPD; Dental office.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400012
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Análisis poblacional de células vegetativas de Bacillus thuringiensis en interacción in vitro con bacterias del suelo Agrociencia
Rojas-Ruiz,Norma E.; Vázquez-Cruz,Candelario; Sánchez-Alonso,Patricia; Sansinenea-Royano,Estibaliz.
La bacteria Bacillus thuringiensis se usa como bioinsecticida agrícola, pero se conoce poco sobre su persistencia en el suelo. El objetivo de este trabajo fue analizar la interacción de las células vegetativas o no esporuladas de B. thuringiensis, con 11 microorganismos nativos de suelo. Las bacterias fueron confrontadas por cocultivo de pares en condiciones controladas, y se evaluaron cinéticas de crecimiento por conteo celular en placa de los microorganismos estudiados. Los resultados mostraron que el desarrollo de B. thuringiensis es afectado por siete de los 11 microorganismos de suelo evaluados. De estos antagonistas, Pseudomonas fluorescens fue uno de los que redujeron más la población de células vegetativas de B. thuringiensis, a 28 °C versus 37 °C;...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antagonismo microbiano; Pseudomonas fluorescens; Klebsiella pneumoniae.
Ano: 2010 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952010000800007
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Peracute bovine mastitis caused by Klebsiella pneumoniae Arq. Bras. Med. Vet. Zootec.
Ribeiro,M.G.; Motta,R.G.; Paes,A.C.; Allendorf,S.D.; Salerno,T.; Siqueira,A.K.; Fernandes,M.C.; Lara,G.H.B..
Relata-se a ocorrência de graves sintomas de mastite hiperaguda em vaca, causada por Klebsiella pneumoniae, na terceira semana de lactação. Descrevem-se aspectos epidemiológicos, sintomas clínicos, procedimentos de diagnóstico microbiológico, conduta terapêutica e medidas de controle.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle; Milk; Klebsiella pneumoniae; Endotoxin; Lipopolysaccharide.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200031
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Perfil de resistência antimicrobiana de Klebsiella pneumoniae isoladas de animais domésticos e silvestres Arq. Bras. Med. Vet. Zootec.
Sousa,A.T.H.I.; Makino,H.; Bruno,V.C.M.; Candido,S.L.; Nogueira,B.S.; Menezes,I.G.; Nakazato,L.; Dutra,V..
RESUMO Klebsiella pneumoniae é um patógeno oportunista, responsável por diversos tipos de infecções nosocomiais, e é considerado um microrganismo multirresistente. Dados na literatura que forneçam informações a respeito da resistência desse microrganismo a antimicrobianos em amostras de animais são escassos. Dessa forma, o objetivo deste trabalho foi avaliar o perfil e o seu aumento das resistências a antimicrobianos dentro da medicina veterinária. Um total de 67 isolados de K. pneumoniae, provenientes de diferentes sítios de isolamento de animais domésticos (39/67) e silvestres (28/67), foi confirmado por sequenciamento do gene 16S rRNA. O maior percentual de isolamento de K. pneumoniae foi de amostras de urina, com 16% (11/67), fezes, com 15% (10/67), e...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella pneumoniae; Multirresistência; Perfil de resistência; MRD.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200584
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An outbreak of acute bovine mastitis caused by Klebsiella pneumoniae in a dairy herd Arq. Bras. Med. Vet. Zootec.
Silva,N.; Costa,G.M..
An outbreak of coliform mastitis is described in a dairy herd from the State of Rio de Janeiro, Brazil. During a four-month period 14 fatal cases of Klebsiella pneumoniae-related mastitis were observed in a herd of 104 lactating cows. The symptoms included peracute enterotoxemia in which the cows died 6 to 12 h after the detection of mastitis by CMT. Staphylococcus aureus and Streptococcus agalactiae Streptococcus agalactiae were also isolated although could not be associated with cases of acute fatal mastitis. Milking practices were also evaluated. The milking machine was being used correctly and adequate precautions for hygiene and pre-milking and post-milking teat dipping were used. The organism was sensitive to gentamicin. Therapy for acute toxic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dairy cow; Acute bovine mastitis; Outbreak; Klebsiella pneumoniae; Vaccination; Treatment.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000400001
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Risk factors for KPC-producing Klebsiella pneumoniae bacteremia BJID
Tuon,Felipe F.; Rocha,Jaime L.; Toledo,Paula; Arend,Lavinia N.; Dias,Camila H.; Leite,Talita M.; Penteado-Filho,Sergio R.; Pilonetto,Marcelo; Zavascki,Alexandre P..
The molecular epidemiology of carbapenemase-producing Klebsiella pneumoniae (KPC) has been largely investigated, but limited clinical information is available. A case-control study was performed to evaluate the risk factors for KPC bacteremia in hospitalized patients. Cases were patients with KPC bacteremia and controls were patients with non-KPC bacteremia. A total of 85 patients were included, 18 (21.2%) were KPC, and 67 (78.8%) were non-KPC (40 [59.7%] of them were extended-spectrum beta-lactamase producers). All KPC isolates were type 2 producers. These isolates belong to five distinct clones. Multivariate analysis showed that age (odds ratio [OR], 1.06; 95% confidence interval [CI], 1.02 - 1.11; p = 0.004), presence of mechanical ventilation (OR,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella pneumoniae; Bacteremia risk factors; Klebsiella pneumoniae; Carbapenemases; Carbapenems.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000500002
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Klebsiella pneumoniae with multiple antimicrobial resistance BJID
Mendes,Caio; Kiffer,Carlos; Segura,Adília; Ribeiro,Julival; Turner,Philip.
A Klebsiella pneumoniae strain was isolated from the urine of a patient at one of the centers participating in the 2001 edition of the MYSTIC program in Brazil. The initial phenotypic findings of the isolated K. pneumoniae presented an unusual MIC of 8 mug/mL to meropenem, 2 mug/mL to imipenem, elevated MICs to broad spectrum cephalosporins (ceftazidime/cefotaxime/cefepime MIC > 256 mug/mL), aminoglycosides (gentamycin > 256 mug/mL and tobramycin = 48 mug/mL), piperacillin/tazobactam (MIC > 256 mug/mL) and susceptibility to ciprofloxacin (MIC = 0.25 mug/mL). The strain also tested positive for ESBL production with double-disk and E-test methodologies. More detailed investigation revealed that the strain produced a SHV-4 type enzyme and also lacked...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Klebsiella pneumoniae; Drug effects; Drug resistance; Carbapenems.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000100008
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Risk factors for infection by extended-spectrum beta-lactamase producing Klebsiella pneumoniae in a tertiary hospital in Salvador, Brazil BJID
Silva,Nanci; Oliveira,Márcio; Bandeira,Antonio Carlos; Brites,Carlos.
Nosocomial infection caused by extended-spectrum beta-lactamase producing Klebsiella pneumoniae (ESBL-Kp) have been frequently reported worldwide. We have no information on such problems in Bahia, Brazil. OBJECTIVES: Evaluate the risk factors for nosocomial infections caused by ESBL-Kp, in a tertiary hospital, in Bahia, Brazil. MATERIAL AND METHODS: We evaluated all reported cases of nosocomial infections caused by ESBL-Kp in a private, tertiary hospital, in Salvador, Brazil, from 2000 through 2004. We compared patients with a diagnosis of ESBL-Kp (cases) and patients infected by non-ESBL producing K. pneumoniae (controls). Mean age, underlying disease, and frequency of invasive procedures were compared between the two groups. History of previous use of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nosocomial infections; ESBL; Klebsiella pneumoniae; Bacterial resistance.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000300007
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Characterization of the genetic environment of the bla KPC-2 gene among Klebsiella pneumoniae isolates from a Chinese Hospital BJID
Shen,Pinghua; Zhang,Ying; Li,Gang; Jiang,Xiaofei.
Abstract Infection caused by carbapenem-resistant Klebsiella pneumoniae has become a major healthcare threat and KPC-2 enzyme is a dominant factor mediating carbapenems resistance in K. pneumoniae. This study was designed to determine the genetic environment of bla KPC-2, which prevailed in clinical K. pneumoniae isolates recovered in Huashan Hospital, Shanghai, China. Forty-two clinical isolates were included in this study by bla KPC-2 screening. After multilocus sequence typing and plasmid analyses of PCR-based replicon typing (PBRT), junction PCR, mapping PCR and crossing PCR assays, primer walking, and amplicon sequencing were used to analyze the genetic environment of the bla KPC-2 gene. ST423, ST65, ST977, and ST11 were all detected in...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Klebsiella pneumoniae; BlaKPC; Genetic environment.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000400384
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Fatal case of donor-derived colistin-resistant carbapenemase-producing Klebsiella pneumoniae transmission in cardiac transplantation BJID
Galvão,Lisia Miglioli; Oliveira,Anna Paula Romero de; Ibanês,Aline Santos; Monteiro,Jussimara; Inoue,Fernanda; Dantas,Daniel Chagas; Sanchez,Flavio; Santos,Daniel Wagner; Abboud,Cely Saad.
ABSTRACT Herein we report a fatal case of donor-derived transmission of XDR-resistant carbapenemase-producing Klebsiella pneumoniae (KPC-Kp) in cardiac transplantation. A 59-year-old male patient with non-obstructive hypertrophic cardiomyopathy underwent heart transplantation. On day 5 post-operation, blood cultures from the donor were positive for colistin-resistant carbapenemase-producing K. pneumoniae (ColR KPC-Kp) susceptible only to amikacin. Recipient blood cultures were also positive for ColR KPC-Kp with the same sensitivity profile as the donor isolate with an identical PFGE pattern. The patient was treated with double-carbapenems and amikacin. The patient evolved to pericarditis, osteomyelitis, and pulmonary necrosis, all fragment cultures...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Cardiac transplantation; Klebsiella pneumoniae; Donor-derived infection; Carbapenem-resistant.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000300235
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Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil BJID
Chagas,Thiago Pavoni Gomes; Alves,Ronaldo Mendes; Vallim,Deyse Christina; Seki,Liliane Miyuki; Campos,Leila Carvalho; Asensi,Marise Dutra.
OBJECTIVE: The present study was undertaken to characterize CTX-M ESBL-producing Klebsiella pneumoniae collected from hospitals in different cities of Brazil. MATERIAL AND METHODS: Eighty-five K. pneumoniae strains isolated from hospitalized patients in six different hospitals of three cities of Brazil were analyzed. ESBL production was confirmed by the standard double-disk synergy test and the Etest®. The MIC50 and MIC90 for ESBL-producing isolates were determined by the Etest® method. The antimicrobial susceptibilities of bacterial isolates were determined using the agar diffusion method according to the CLSI. Screening for blaTEM, blaSHV, blaCTX-M genes and class 1 integron was performed by PCR amplification. To determine the genomic diversity of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella pneumoniae; Β-lactamases; Genotype.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000500002
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Klebsiella ESBL bacteremia-mortality and risk factors BJID
Tuon,Felipe Francisco; Kruger,Margot; Terreri,Marcos; Penteado-Filho,Sergio R; Gortz,Lucas.
Background: Extended spectrum β-lactamase (ESBL)-producing bacteria have become recognized as a problem in South America. The aim of this study was to evaluate risk factors and mortality rate in bacteremia caused by ESBL-producing Klebsiella pneumoniae in a Brazilian hospital. Methods: A three-year retrospective cohort study with 104 cases of K. pneumoniae bacteremia (61 ESBL and 43 non-ESBL). Several clinical and laboratory variables were evaluated. The outcome of interest was 30-day mortality. The adequate treatment was evaluated according to antibiotic susceptibility. Results: Multivariable analysis showed that central venous catheter and mechanical ventilation were independent risk factors for ESBL. The duration of hospitalization before the bacteremia...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Klebsiella pneumoniae; Beta-lactamases; Bacteremia; Carbapenems.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000600016
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Differential activity of a lectin from Solieria filiformis against human pathogenic bacteria BJMBR
Holanda,M.L.; Melo,V.M.M.; Silva,L.M.C.M.; Amorim,R.C.N.; Pereira,M.G.; Benevides,N.M.B..
A lectin isolated from the red alga Solieria filiformis was evaluated for its effect on the growth of 8 gram-negative and 3 gram-positive bacteria cultivated in liquid medium (three independent experiments/bacterium). The lectin (500 µg/mL) stimulated the growth of the gram-positive species Bacillus cereus and inhibited the growth of the gram-negative species Serratia marcescens, Salmonella typhi, Klebsiella pneumoniae, Enterobacter aerogenes, Proteus sp, and Pseudomonas aeruginosa at 1000 µg/mL but the lectin (10-1000 µg/mL) had no effect on the growth of the gram-positive bacteria Staphylococcus aureus and B. subtilis, or on the gram-negative bacteria Escherichia coli and Salmonella typhimurium. The purified lectin significantly reduced the cell density...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Red alga; Solieria filiformis; Human pathogenic bacteria; Lectin; Antibacterial activity; Klebsiella pneumoniae.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200005
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Rapid, specific, and sensitive detection of the ureR_1 gene in Klebsiella pneumoniae by loop-mediated isothermal amplification method BJMBR
Li,Chao; Fu,Gongyu; Shi,Yaoqiang; Zhang,A-Mei; Xia,Xueshan; Fang,Yue; Mao,Xiaoqin; Jiang,Jie; Song,Yuzhu; Yang,Guangying.
Klebsiella pneumoniae is one of the main pathogenic bacteria that causes nosocomial infections, such as pneumonia, urinary tract infection, and sepsis. Therefore, the rapid and accurate detection of K. pneumoniae is important for the timely treatment of infectious patients. This study aimed to establish a loop-mediated isothermal amplification (LAMP) method for the rapid and sensitive detection of K. pneumoniae-specific gene ureR_1 (Gene ID: 11847803). The ureR_1 gene was obtained through local and online BLAST, and the specific primers were designed for its detection. Positive reactions were observed on all 140 K. pneumoniae clinical isolates while all the 82 non-K. pneumoniae clinical isolates were negative. Plasmids with the specific gene and the mouse...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Loop-mediated isothermal amplification; Polymerase chain reaction; Klebsiella pneumoniae; Novel specific gene; Specific; Sensitive method.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000300609
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Genetic profiling of Klebsiella pneumoniae: comparison of pulsed field gel electrophoresis and random amplified polymorphic DNA BJM
Ashayeri-Panah,Mitra; Eftekhar,Fereshteh; Ghamsari,Maryam Mobarak; Parvin,Mahmood; Feizabadi,Mohammad Mehdi.
In this study, the discriminatory power of pulsed field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) methods for subtyping of 54 clinical isolates of Klebsiella pneumoniae were compared. All isolates were typeable by RAPD, while 3.6% of them were not typeable by PFGE. The repeatability of both typing methods were 100% with satisfying reproducibility (≥ 95%). Although the discriminatory power of PFGE was greater than RAPD, both methods showed sufficient discriminatory power (DI > 0.95) which reflects the heterogeneity among the K. pneumoniae isolates. An optimized RAPD protocol is less technically demanding and time consuming that makes it a reliable typing method and competitive with PFGE.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella pneumoniae; Pulsed field gel electrophoresis; Random amplified polymorphic DNA; Genotyping.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300024
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Performance of “RESIST-3 O.K.N. K-SeT” immunochromatographic assay for the detection of OXA-48 like, KPC, and NDM carbapenemases in Klebsiella pneumoniae in Turkey BJM
Sağıroğlu,Pınar; Hasdemir,Ufuk; Gelmez,Gülşen Altınkanat; Aksu,Burak; Karatuna,Onur; Söyletir,Güner.
ABSTRACT In this study, the performance of the “RESIST-3 O.K.N. K-SeT” (Coris BioConcept, Gembloux, Belgium) immunochromatographic assay was evaluated in 132 Klebsiella pneumoniae comprising 102 carbapenem resistant and 30 carbapenem susceptible isolates. Genotypically known isolates of Gram negative bacteria (n = 22) including various species were also tested by the assay as controls. The isolates tested by the immunochromatographic assay and also were run PCR for bla KPC, bla IMP, bla VIM, bla NDM, and bla OXA-48. The rates of bla NDM, bla OXA-48, and bla KPC in carbapenem resistant isolates were found at 52.9%, 39.2%, and 2.0%, respectively. Both bla NDM and bla OXA-48 were found in six (5.9%) isolates. The results of the assay showed 100% concordance...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella pneumoniae; Immunochromatographic test; NDM; KPC; OXA-48.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400885
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Atypical phenotypic characteristics of klebsiella pneumoniae isolates from an outbreak in a neonatal intensive care unit in Brazil BJM
Otman,J.; Perugini,M.E.; Tognim,M.C.B.; Vidotto,M.C..
Extended-spectrum beta-Lactamase-producing (ESBL) Klebsiella sp.isolates from an outbreak in a Neonatal Intensive Care Unit (NICU) at a teaching hospital in Londrina, Paraná State, Brazil, presented atypical phenotypic characteristics that hampered their identification and the distinction between Klebsiella and Enterobacter species. Ten isolates were identified as K. pneumoniae due to negative reactions for motility and inducible beta-lactamase test (ESBL and AmpC) despite being positive for ornithyne descarboxilase. These isolates were genotyped by ribotyping and polymerase chain reaction (PCR) with repetitive extragenic palindromic sequences (REP). Ribotyping by means of an automated instrument and EcoRI and Pvu II as restriction enzymes resulted...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella pneumoniae; REP-PCR; Genetic variation; Ornithinolytic.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200016
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Resistance of Klebsiella Pneumoniae clinical isolates: linkage of outer membrane proteins (omps) with production esbls BJM
Marques,Lívia Érika Carlos; Oliveira,Danielle Ferreira de; Marques,Márcia Maria Mendes; Silva,Ana Raquel Araújo da; Alves,Carlucio Roberto; Guedes,Maria Izabel Florindo.
Three isolates of Klebsiella pneumoniae, collected from the University Hospital in Fortaleza, Brazil, were analyzed to determine their resistance to multiple antibiotics. The results of this study showed that the resistance of the clinically isolated bacteria is associated with the production of extended-spectrum beta-lactamases (ESLBs) and loss of outer membrane proteins.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella pneumoniae; Β-lactamase and porin.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200009
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Integron mediated multidrug resistance in extended spectrum beta-lactamase producing clinical isolates of Klebsiella pneumoniae BJM
Mobarak-Qamsari,Maryam; Ashayeri-Panah,Mitra; Eftekhar,Freshteh; Feizabadi,Mohammad Mehdi.
The present study describes integron mediated multiple antibiotic resistance in extended-spectrum β-lactamase producing clinical isolates of Klebsiella pneumoniae. One hundred and four clinical isolates of K. pneumoniae from two Iranian hospitals were screened for extended-spectrum β-lactamase production and susceptibility of the extended-spectrum β-lactamase producing isolates was determined to 17 antibiotics by disc diffusion. Presence of integron classes 1, 2 and 3 was detected by PCR and integrase specific primers. Isolates harboring class 1 integron were then screened for variable regions using PCR. Fifty isolates (48%) produced extended-spectrum β-lactamases among which, 22 (44%) harbored class 1, 3 (6%) carried class 2 and none contained class 3...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella pneumoniae; Extended-spectrum β-lactamase; Multiple antibiotic resistance; Integron.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300028
Registros recuperados: 27
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