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Metabolomics based on UHPLC-QToF- and APGC-QToF-MS reveals metabolic pathways reprogramming in response to tidal cycles in the sub-littoral species Mimachlamys varia exposed to aerial emergence ArchiMer
Ory, Pascaline; Bonnet, Antoine; Mondeguer, Florence; Breitwieser, Marine; Dubillot, Emmanuel; Graber, Marianne.
Mimachlamys varia is a sub-littoral bivalve encountered from Norway to the Mediterranean Sea, which lives mostly byssally attached to rocks. During the low tide period, M. varia individuals, located highest on the shore, may experience short time of aerial exposure and face a low availability of oxygen. Here we report a comparative metabolomic profiling of gill samples of M. varia obtained by both LC-QToF and APGC-QToF mass spectrometry, to analyze metabolic changes occurring during emersion in comparison with immersion. Scallops were grown in aquaria with a simulated intertidal environment mimicking short-duration air exposure that they might experience during extreme tides: alternating 2 h emersion and 10 h immersion. Our results show a switch from...
Tipo: Text Palavras-chave: Anaerobic; APGC-QToF mass spectrometry; Hypoxia; Intertidal; LC-QToF mass spectrometry; Metabolomics; Mimachlamys varia; PLS-DA.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00466/57741/59936.pdf
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