|
|
| Ragonezi,Carla; Caldeira,A. Teresa; Rosário Martins,M.; Salvador,Cátia; Santos-Silva,Celeste; Ganhão,Elsa; Klimaszewska,Krystyna; Zavattieri,Amely. |
| Stone pine (Pinus pinea L.), like other conifers, forms ectomycorrhizas (ECM), which have beneficial impact on plant growth in natural environments and forest ecosystems. An in vitro co-culture of stone pine microshoots with pure mycelia of isolated ECM sporocarps was used to overcome the root growth cessation not only in vitro but also to improve root development during acclimation phase. Pisolithus arhizus (Scop.) Rauschert and Lactarius deliciosus (L. ex Fr.) S.F. Gray fungi, were collected, pure cultured and used in in vitro co-culture with stone pine microshoots. Samples of P. arhizus and L. deliciosus for the in vitro co-cultures were collected from the pine stands southwest Portugal. The in situ characterization was based on their morphotypes. To... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pisolithus arhizus; Lactarius deliciosus; Pinus pinea; M13-PCR; ITS. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200047 |
| |
