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Registros recuperados: 74
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Efecto de los niveles de Vitafert y melaza en la pollinaza fermentada aeróbica. Colegio de Postgraduados
Arias López, Flor de Tila.
La pollinaza es la excreta de las aves de engorda y se ha utilizado en la suplementación de los bovinos debido al elevado contenido de nitrógeno no proteínico (NNP) y por su bajo costo. Con el objetivo de mejorar los parámetros bromatológicos y fermentativos de la pollinaza. Se estudió en diseño completamente al azar con arreglo factorial 3 x 3 con 3 repeticiones por tratamientos, con variable de respuesta incremento en Y, el efecto de los niveles de Vitafert (0, 30 y 60%) y melaza (10, 20, y 30%) Se mezclo la pollinaza con los porcentajes de Vitafert y melaza de forma homogénea, se utilizo 1kg de muestra como unidad experimental agregando agua a los tratamientos para mantener una proporción del 60 % de humedad, una vez mezclados los ingredientes...
Palavras-chave: Pollinaza; Vitafert; Melaza; Proteína cruda; Proteína verdadera; PH; Bacteria ácido láctica; Ooquiste; Manure; Molasses; Protein raw; True protein; Lactic acid bacteria; Oocyst; Maestría; Producción Agroalimentaria en el Trópico.
Ano: 2010 URL: http://hdl.handle.net/10521/287
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High density process to cultivate Lactobacillus plantarum biomass using wheat stillage and sugar beet molasses Electron. J. Biotechnol.
Krzywonos,Magorzata; Eberhard,Thomas.
Background: Owing to the growing interest in biofuels, the concept of a biorefinery where biomass is converted to a variety of useful products is gaining ground. We here present how distillery waste is combined with a by-product from a sugar production, molasses, to form a medium for the growth of Lactobacillus plantarum with yields and biomass densities comparable with conventional industrial media. Such approach enables a cost-effective utilization of the problematic wastewater from ethanol and a by-product from sugar production. It is the first approach that attempts to find low-cost media for the production of Lactobacillus plantarum biomass. Results: This study suggests that sieved wheat stillage enriched by adding 1.77 g/l yeast extract and 10%...
Tipo: Journal article Palavras-chave: Distillery wastewater; High density fermentation; Lactic acid bacteria; Lactobacillus plantarum; Sugar beet molasses; Wheat stillage.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200006
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Safe use of genetically modified lactic acid bacteria in food: Bridging the gap between consumers, green groups, and industry Electron. J. Biotechnol.
Sybesma,Wilbert; Hugenholtz,Jeroen; de Vos,Willem M.; Smid,Eddy J..
Within the European Union (EU), the use of genetically modified organisms (GMOs) in food production is not widely applied and accepted. In contrast to the United States of America, the current EU legislation limits the introduction of functional foods derived from GMOs that may bring a clear benefit to the consumer. Genetically modified lactic acid bacteria (GM-LAB) can be considered as a different class of GMOs, and the European Union is preparing regulations for the risk assessment of genetically modified microorganisms. Since these procedures are not yet implemented, the current risk assessment procedure is shared for GMOs derived from micro organisms, plants, or animals. At present, the use of organisms in food production that have uncontrolled genetic...
Tipo: Journal article Palavras-chave: Lactic acid bacteria; Genetic modification; Legislation; Novel foods; Safety assessment.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400011
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Effects of fermentation substrates and conservation methods on the viability and antimicrobial activity of Weissella confusa and its metabolites Electron. J. Biotechnol.
Serna-Cock,Liliana; Enríquez-Valencia,Cruz E; Jiménez-Obando,Eliana M; Campos-Gaona,Rómulo.
Lactic acid bacteria produce metabolites with antagonistic activity against other bacteria. However, growth conditions and conservation methods may reduce the viability and antimicrobial activity of lactic acid bacteria. This study evaluated the effects of fermentation substrate, lyophilization (freeze-drying) and refrigeration on the viability and antimicrobial activity of Weissella confusa strain and its metabolites against pathogens responsible for bovine mastitis. W. confusa strain was grown in MRS broth and milk supplemented with yeast extract and glucose (MYEG). The collected fractions were preserved by lyophilization or under refrigeration at 4ºC. Every seven days, the viability of W. confusa strain and the stability of its metabolites were...
Tipo: Journal article Palavras-chave: Conservation; Inhibition; Lactic acid bacteria; Lyophilization.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300006
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Selection and evaluation of seafood-borne psychrotrophic lactic acid bacteria as inhibitors of pathogenic and spoilage bacteria ArchiMer
Matamoros, Sebastien; Pilet, Marie-france; Gigout, Frederique; Prevost, Herve; Leroi, Francoise.
In this study, inhibitory psychrotrophic lactic acid bacteria were isolated and investigated for future use in biopreservation of seafood products. Screening of 5575 colonies isolated from various seafood products resulted in the selection of 132 colonies presenting inhibitory properties. Among them, 52 isolates had characteristics of LAB and showed growth at 15 °C but not at 30 °C. The inhibition spectrum of these 52 isolates against 14 target strains (Gram-positive and -negative) showed inhibition of typical seafood spoiling and pathogenic bacteria and enabled the formation of seven interesting clusters. Sequencing of the 16S rRNA gene of a representative isolate from each cluster identified three Leuconostoc gelidum, two Lactococcus piscium, one...
Tipo: Text Palavras-chave: Carnobacterium alterfunditum; Lactobacillus fuchuensis; Leuconostoc gelidum; Lactococcus piscium; Psychrotrophic; Inhibition; Seafood; Lactic acid bacteria.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6472.pdf
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Selection of lactic acid bacteria as candidate probiotics and in vivo test on Artemia nauplii ArchiMer
Lamari, Faouzi; Sadok, Khouadja; Bakhrouf, Amina; Gatesoupe, Joel.
Lactic acid bacteria (LAB) were isolated from rotifer cultures in a marine hatchery to search for potential probiotics for marine animals. Fifteen strains were first selected among a total of 55, according to antibacterial activity against Vibrio sp. Among eight strains identified as Lactobacillus casei, four were highly adhesive, suggesting some ability for surface colonization. The other strains were identified as Lactobacillus plantarum, Lactobacillus dextrinicus, and Leuconostoc sp. To validate probiotic potential, Artemia were challenged against pathogenic Vibrio alginolyticus, with or without one of six selected LAB strains. The six strains protected Artemia against the pathogen, to some extent on condition that nutrient enrichment was provided. La....
Tipo: Text Palavras-chave: 16S rDNA; Antagonism; Artemia; Lactic acid bacteria; Vibrio.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00154/26511/24767.pdf
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Quantification des bactéries histaminogènes et maîtrise de la formation d'histamine dans les produits marins par biopréservation ArchiMer
Podeur, Gaetan.
Histamine-producing bacteria were isolated from naturally contaminated seafood, some of responsible for histamine-poisoning. At the same time, lactic acid bacteria were isolated on the same products and clustered according to their inhibitory activity against 4 histamine-producing species. Most of the strains were identified as Lactobacillus fuchuensis or Lactobacillus sakei. Twelve lactic acid bacteria were selected for challenge-test on canned, cooked or smoked tuna. Challenge-test combined with sensory analysis performed on cooked vacuum-packed tuna against Morganella psychrotolerans and Morganella morganii, demonstrated an important inhibitory effect on growth and production of histamine with L. sakei CNCM I-4707. Bacterial count was reduced by 3 to 5...
Tipo: Text Palavras-chave: Biopréservation; Thon; Bactérie lactique; Intoxication histaminique; Morganella morganii; Morganella psychrotolerans; Lactobacillus sakei; Analyse sensorielle; PCR en temps réel; Étape d'enrichissement; RT-qPCR; Biopreservation; Tuna; Lactic acid bacteria; Scombroid poisoning; Morganella morganii; Morganella psychrotolerans; Lactobacillus sakei; Sensory analysis; Real-time PCR; Enrichment step; RT-qPCR.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00241/35203/33709.pdf
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Applications of protective cultures, bacteriocins and bacteriophages in fresh seafood and seafood products ArchiMer
Pilet, Marie-france; Leroi, Francoise.
Microbial seafood-borne disease represents 10 to 20% of the total food-borne outbreaks. Most of them are from bacterial origin and involve seafood products that have been contaminated by pathogenic Vibrio, Listeria monocytogenes and histaminogen bacteria. On the other hand, seafood products are very sensitive to the development of spoiling bacteria producing off-odours. Pathogenic and spoiling microflora are not always reduced or limited by the processing steps that are currently used in these foodstuffs, and the interest for alternative techniques such as bioprotection to improve quality and safety of seafood has increased in the last years. Among the microbial flora of lightly preserved seafood products, lactic acid bacteria usually become dominant...
Tipo: Text Palavras-chave: Biopreservation of seafood; Seafood safety and spoilage; Lactic acid bacteria; Sensory quality; Listeria monocytogenes.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00030/14138/11384.pdf
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Selection of bioprotective cultures for preventing cold-smoked salmon spoilage ArchiMer
Leroi, Francoise; Cornet, Josiane; Chevalier, Frederique; Cardinal, Mireille; Coeuret, Gwendoline; Chaillou, Stephane; Joffraud, Jean-jacques.
Biopreservation is a natural technology of food preservation, which consists of inoculating food with microorganisms selected for their antibacterial properties. The objective of this study was to select lactic acid bacteria (LAB) to improve the quality of cold-smoked salmon (CSS). In this work, different strains representative of the 4 dominant species, identified in a previous study by pyrosequencing the 16S rRNA gene, were isolated and their spoiling potential in CSS blocks, sterilized by ionization, was assessed by twelve trained panelists along the vacuum storage at 8 °C . Photobacterium phosphoreum, Brochothrix thermosphacta and Serratia proteamaculans released strong off-odors whereas the spoiling potential of Carnobacterium divergens was weaker....
Tipo: Text Palavras-chave: Biopreservation; Lactic acid bacteria; Sensory analysis; Seafood products; Microbial ecosystem; Pyrosequencing.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00268/37966/36045.pdf
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Updating the importance of lactic acid bacteria in fish farming: natural occurrence and probiotic treatments ArchiMer
Gatesoupe, Joel.
Many recent papers have deepened the state of knowledge about lactic acid bacteria (LAB) in fish gut. In spite of high variability in fish microbiota, LAB are sometimes abundant in the intestine, notably in freshwater fish. Several strains of Streptococcus are pathogenic to fish. S. iniae and Lactococcus garvieae are major fish pathogens, against which commercial vaccines are available. Fortunately, most LAB are harmless, and some strains have been reported for beneficent effects on fish health. A major step forward of the recent years was those converging evidences that LAB can stimulate the immune system in fish. An open question is whether viability can affect immunostimulation. The issue is crucial to commercialise live probiotics rather than...
Tipo: Text Palavras-chave: Probiotic; Pathogen; Lactic acid bacteria; Gastrointestinal microbiota; Fish.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2007/publication-2589.pdf
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Influence of temperature, pH and NaCl concentration on the maximal growth rate of Brochothrix thermosphacta and a bioprotective bacteria Lactococcus piscium CNCM I-4031 ArchiMer
Leroi, Francoise; Fall, Papa-abdoulaye; Pilet, Marie-france; Chevalier, Frederique; Baron, Regis.
The maximum specific growth rate (μmax) of Brochothrix thermosphacta, a spoilage bacteria of cooked peeled shrimp, and Lactococcus piscium CNCM I-4031, a bioprotective strain, was investigated under different conditions of temperature, NaCl concentrations and pH. The basic modelling approach used was the Gamma concept (γ-concept) and the model developed was then adapted to shrimp. Cardinal growth parameters were quite similar for the two strains, except for NaCl. No NaCl was required for growth and the NaClmax was three-times higher for B. thermosphacta than for L. piscium (62 and 23 g l−1 respectively). However, tolerance to NaCl was higher in seafood than in liquid broth, possibly due to presence of osmoltically active molecules. L. piscium and B....
Tipo: Text Palavras-chave: Modelling; Biopreservation; Shrimp; Lactic acid bacteria; Brochothrix thermosphacta; Spoilage bacteria; Environmental conditions.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00083/19473/17088.pdf
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Occurrence and role of lactic acid bacteria in seafood products ArchiMer
Leroi, Francoise.
Lactic acid bacteria (LAB) in fish flesh has long been disregarded because the high post-mortem pH, the low percentage of sugars, the high content of low molecular weight nitrogenous molecules and the low temperature of temperate waters favor the rapid growth of pH-sensitive psychrotolerant marine Gram-negative bacteria like Pseudomonas, Shewanella and Photobacterium. In seafood packed in both vacuum (VP) and modified atmosphere (MAP) packaging commonly CO2 enriched, the growth of the Gram-negative aerobic bacteria group (predominantly pseudomonads) is effectively inhibited and the number reached by LAB during storage is higher than that achieved in air but always several log units lower than the trimethylamine oxide (TMA-O) reducing and CO2-resistant...
Tipo: Text Palavras-chave: Lactic acid bacteria; Fish; Spoilage; Biopreservation; Probiotic; Fermentation.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00006/11750/8447.pdf
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Maîtrise du développement de Listeria monocytogenes dans le saumon fumé : intérêt de la biopréservation par des bactéries lactiques ArchiMer
Richard, Christelle; Leroi, Francoise; Brillet-viel, Anne; Rachman, Cinta; Connil, Nathalie; Drider, Djamel; Pilet, Marie-france; Onno, Bernard; Dousset, Xavier; Prevost, Hervé.
The interest of biopreservation using lactic bacteria to improve the food security of smoked salmon was evaluated. Cold smoked salmon is a non-stable microbiological product whose physicochemical characteristics allow the development of Listeria monocytogenes which constitutes the major microbiological risk depending on the consumption of this product. Biodiversity of the microflora of smoked salmon and its evolution during conservation, the origin of the contamination by Listeria and its consequences, and the parameters of the manufacturing process allowing a better control of microbiological quality are discussed. The innovating concept of biopreservation of smoked salmon is developed. It consists of an inoculation of salmon by a competitive lactic flora...
Tipo: Text Palavras-chave: Saumon fumé; Bactérie lactique; Biopréservation; Listeria; Carnobacterium; Cold smoked salmon; Lactic acid bacteria; Biopreservation; Listeria; Carnobacterium.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/00000/10768/7509.pdf
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Lactococcus piscium : a psychotrophic lactic acid bacterium with bioprotective or spoilage activity in food - a review ArchiMer
Saraoui, Taous; Leroi, Francoise; Bjorkroth, J.; Pilet, M. F..
The genus Lactococcus comprises twelve species, some known for decades and others more recently described. Lactococcus piscium, isolated in 1990 from rainbow trout, is a psychrotrophic lactic acid bacterium (LAB), probably disregarded because most of the strains are unable to grow at 30°C. During the last 10 years, this species has been isolated from a large variety of food: meat, seafood and vegetables, mostly packed under vacuum (VP) or modified atmosphere (MAP) and stored at chilled temperature. Recently, culture-independent techniques used for characterization of microbial ecosystems have highlighted the importance of L. piscium in food. Its role in food spoilage varies according to the strain and the food matrix. However, most studies have indicated...
Tipo: Text Palavras-chave: Biopreservation; Cold adaptation; Lactic acid bacteria; Meat; Seafood; Spoilage.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00334/44517/44213.pdf
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New Insight into Antimicrobial Compounds from Food and Marine-Sourced Carnobacterium Species through Phenotype and Genome Analyses ArchiMer
Begrem, Simon; Ivaniuk, Flora; Gigout Chevalier, Frederique; Kolypczuk, Laetitia; Bonnetot, Sandrine; Leroi, Francoise; Grovel, Olivier; Delbarre-ladrat, Christine; Passerini, Delphine.
Carnobacterium maltaromaticum and Carnobacterium divergens, isolated from food products, are lactic acid bacteria known to produce active and efficient bacteriocins. Other species, particularly those originating from marine sources, are less studied. The aim of the study is to select promising strains with antimicrobial potential by combining genomic and phenotypic approaches on large datasets comprising 12 Carnobacterium species. The biosynthetic gene cluster (BGCs) diversity of 39 publicly available Carnobacterium spp. genomes revealed 67 BGCs, distributed according to the species and ecological niches. From zero to six BGCs were predicted per strain and classified into four classes: terpene, NRPS (non-ribosomal peptide synthetase), NRPS-PKS (hybrid...
Tipo: Text Palavras-chave: Lactic acid bacteria; Antimicrobial activity; Carnobacteriumspp; Hydrogen peroxide; Bacteriocin; RiPP; NRPS; Terpene; Natural product; Genome mining.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00641/75335/75999.pdf
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白かびを接種した発酵ソーセージの製造と諸性質 OAK
三上, 正幸; DORJ, Serjmyadag; 島田, 謙一郎; 関川, 三男; 福島, 道広; 山岸, 真; 山腰, 和枝; 大美浪, 源; MIKAMI, Masayuki; SHIMADA, Ken-ichiro; SEKIKAWA, Mitsuo; FUKUSHIMA, Michihiro; YAMAGISHI, Makoto; YAMAKOSHI, Kazue; OHMINAMI, Hajime.
Fermented sausages were produced using bacterial starter culture and 3 types of mould starter cultures,and investigated their properties. Mould starter cultures were sprayed to surface of sausages at 3 days,and the surfaces were changed to white colour afterwards and another moulds, such as blue or black one,were not observed. Common bacterial and lactic acid bacterial counts were range of 6.6 x 10⁸cfu/g~2.0 x 10⁹cfu/g,and Coliform group,Staphylococcus aureus and Salmonella were not detected in the products at 35 days. pH decreased rapidly at 7 days and pH of products became to 4.6~4.7. Water contents were 30.0~31.6%,and water activity was 0.80~0.81. Nitrite ion concentration at starting time was 147.7ppm,but became to 9.8~13.2ppm in the products. There...
Palavras-chave: 食肉製品; ソーセージ; 発酵ソーセージ; 乳酸菌; かび; Meat products; Sausages; Fermented sausages; Lactic acid bacteria; Mould.
Ano: 2004 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3091
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Lactobacillus fermentum TDS030603株の生産する中性多糖の構造研究 OAK
Leo, Fiame; Hashida, Shukichi; Kumagai, Daiju; Uchida, Kenji; Motoshima, Hidemasa; Arai, Ikichi; Asakuma, Sadaki; Fukuda, Kenji; Urashima, Tadasu; 福田, 健二; 浦島, 匡.
A neutral exopolysaccharide produced in large amounts by Lactobacillus fermentum TDS030603 was purified from culture supernatants and its structure determined by monosaccharide analysis, methylation analysis and 1H-NMR spectroscopy. Optimum conditions for the production of this exopolysaccharide by the above bacterial strain, when cultured using cheese whey medium or MRS broth, were also investigated. The exopolysaccharide was found to be composed of D-galactose and D-glucose in the molar ratio of 1:2.5 and to consist of one major as well as one minor saccharide, both of which contained a tetrasaccharide repeating unit consisting of two α and two β anomers. The major saccharide contained non-reducing glucopyranose, O-3 substituted glucopyranose, O-6...
Palavras-chave: Lactic acid bacteria; Exopolysaccharide; Lactobacillus fermentum TDS030603.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4183
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Effects of carbohydrate source on physicochemical properties of the exopolysaccharide produced by Lactobacillus fermentum TDS030603 in a chemically defined medium OAK
Fukuda, Kenji; Shi, Tala; Nagami, Kentaro; Leo, Fiame; Nakamura, Tadashi; Yasuda, Kumi; Senda, Akitsugu; Motoshima, Hidemasa; Urashima, Tadasu; 福田, 健二; 浦島, 匡.
A thermophilic lactic acid bacterium, Lactobacillus fermentum TDS030603, produced about 100 mg/L of EPS in purified form when grew in de Man-Rogosa-Sharpe (MRS) broth. The 1% (w/v) solution of the purified EPS was highly viscous, exhibiting an apparent 22 viscosity (ηapp) of 0.88 Pa s at a shear rate of 10/s. To investigate the impact of carbohydrate source on the production yield and chemical structure of EPS and the viscosity of EPS solution, a chemically defined medium (CDM) has been developed. Results of TLC, HPLC, 25 and 1H NMR spectroscopy indicated that the chemical structures of EPS released in MRS and in the CDM supplemented with glucose, galactose, lactose or sucrose were very similar. All the 1% solutions of EPSs released in CDMs were highly...
Palavras-chave: Chemical structure; Chemically defined medium; Exopolysaccharide; Lactic acid bacteria; Viscosity.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2694
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醗酵食肉製品に用いる有用細菌の食塩による生育阻害に対するアルギニンの緩和効果 OAK
関川, 三男; 河村, 多美; 藤井, はるか; 島田, 謙一郎; 福島, 道広; 三上, 正幸; SEKIKAWA, M.; KAWAMURA, T.; FUJII, H.; SHIMADA, K.; FUKUSHIMA, M.; MIKAMI, M..
In this study,the effect of arginine (Arg) of the NaCl induced growth inhibition was examined for Sta. carnosus and P.acidilactici which isolated from the commercial starter culture of fermented sausage. When Arg and/or NaCl were added to MRS broth and cultured with shaking,the differences were not recorded in the broth pH,the nitrate reduction activity,and the protein degradation activity. However,the viable count of both strains had decreased by adding NaCl. The addition of Arg has increased the number of recovered cell and it was remarkable in Sta.carnosus. Arg added broth was metabolized by both strain,the content of Arg in broth was decreased, especially it was disappeared in cultured broth of Sta.carnosus. SEM photographs showed an enlarged and...
Palavras-chave: 醗酵食肉製品; 乳酸菌; 食塩; アルギニン; Fermented meat; Lactic acid bacteria; Salt; Arginine.
Ano: 2003 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3092
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豆腐製造過程で生じる豆腐ホエーの利用 OAK
小嶋, 道之; 青山, 将; KOJIMA, Michiyuki; AOYAMA, Masaru.
豆乳100ml に市販の0.03ml 酢酸を加えてオートクレーブ(121℃, 15分間) すると, 大豆タンパ ク質は凝固した。ロ液は, 淡黄色の酸処理豆腐ホエーであり, この酸処理豆腐ホエー(pH4.0前後) で乳酸菌を培養することができた。また, 酸処理した凝固タンパク質は家畜の飼料として利用可能 であった。この酸処理手法は, 豆腐製造過程での廃液(豆腐ホエー) にも利用でき, 未利用資源で ある豆腐ホエーが, 乳酸菌培養用の培地として有効であることを明らかにした。また, 豆腐残査 (OKARA) に添加して, 保存期間の延長が可能であった。 In the current studies, we found that addition of 0.03 ml acetic acid to 100 ml soy milk caused it to solidify when autoclaved for 15 min at 121℃. The whey remaining after this process is light yellow in color, has a pH of approximately 4.0, and acted as an effective medium for culturing lactic acid bacteria. To investigate the potential of the solidified protein as a feed for domestic animals, the protein was fed to a Holstein cow over a week and was usually more than 95% consumed....
Palavras-chave: 豆腐製造; 豆腐ホエー; 酢酸処理; 乳酸菌発酵; オカラ; Tofu making; Tofu whey; Acetic acid treatment; Lactic acid bacteria; Fermentation; OKARA.
Ano: 2004 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/88
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