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	Provedor de dados Ciênc. Tecnol. Aliment. (1) BJMBR (1) Genet. Mol. Biol. (1) Autor BOSSO,Alessandra (1) Bona,Laura Renata de (1) Bulhões,A.C. (1) Francesconi,Carlos Fernando de Magalhães (1) Goldani,H.A.S. (1) Grott,Camila Schultz (1) Lunge,Vagner Ricardo (1) MORIOKA,Luiz Rodrigo Ito (1) Matte,U.S. (1) Mazzoleni,Felipe (1) Mazzoleni,Luiz Edmundo (1) Mazzuca,R.B. (1) Milbradt,Tobias Cancian (1) Nabinger,Débora Dreher (1) Oliveira,F.S. (1) Rech,Tássia Flores (1) SANTOS,Leandro Freire dos (1) SUGUIMOTO,Hélio Hiroshi (1) Sander,Guilherme Becker (1) Silveira,T.R. (1) Mais... Palavra-chave Lactose intolerance (3) Bloating (1) Dyspepsia (1) Enzymatic characterization (1) Enzyme activity (1) Gastrointestinal diseases (1) Hydrogen breath test (1) LCT polymorphism (1) Lactase-phlorizin hydrolase (1) Milk intolerance (1) Single nucleotide polymorphism (1) UHT and skimmed milk (1) Β-galactosidase (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2018 (1) 2016 (1) 2007 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Correlation between lactose absorption and the C/T-13910 and G/A-22018 mutations of the lactase-phlorizin hydrolase (LCT) gene in adult-type hypolactasia BJMBR Bulhões,A.C.; Goldani,H.A.S.; Oliveira,F.S.; Matte,U.S.; Mazzuca,R.B.; Silveira,T.R.. The C/T-13910 mutation is the major factor responsible for the persistence of the lactase-phlorizin hydrolase (LCT) gene expression. Mutation G/A-22018 appears to be only in co-segregation with C/T-13910. The objective of the present study was to assess the presence of these two mutations in Brazilian individuals with and without lactose malabsorption diagnosed by the hydrogen breath test (HBT). Ten milk-tolerant and 10 milk-intolerant individuals underwent the HBT after oral ingestion of 50 g lactose (equivalent to 1 L of milk). Analyses for C/T-13910 and G/A-22018 mutations were performed using a PCR-based method. Primers were designed for this study based on the GenBank sequence. The CT/GA, CT/AA, and TT/AA genotypes (lactase persistence) were found in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Hydrogen breath test; Lactase-phlorizin hydrolase; Lactose intolerance; Milk intolerance; LCT polymorphism. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001100004 Lactose hydrolysis potential and thermal stability of commercial β-galactosidase in UHT and skimmed milk Ciênc. Tecnol. Aliment. BOSSO,Alessandra; MORIOKA,Luiz Rodrigo Ito; SANTOS,Leandro Freire dos; SUGUIMOTO,Hélio Hiroshi. Abstract The commercial enzyme (E.C. = 3.2.1.23) from Kluyveromyces lactis (liquid) and Aspergillus oryzae(lyophilized) was investigated for its hydrolysis potential in lactose substrate, UHT milk, and skimmed milk at different concentrations (0.7; 1.0 and 1.5%), pH values (5.0; 6.0; 6.5 and 7.0), and temperature (30; 35; 40 and 55 ºC). High hydrolysis rates were observed for the enzyme from K. lactis at pH 7.0 and 40 ºC, and from A. oryzae at pH 5.0 and 55 ºC. The enzyme from K. lactis showed significantly higher hydrolysis rates when compared to A. oryzae. The effect of temperature and β-galactosidase concentration on the lactose hydrolysis in UHT milk was higher than in skimmed milk, for all temperatures tested. With respect to the thermal stability, a... Tipo: Info:eu-repo/semantics/article Palavras-chave: UHT and skimmed milk; Β-galactosidase; Enzymatic characterization; Lactose intolerance; Enzyme activity. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000100159 The association between adult-type hypolactasia and symptoms of functional dyspepsia Genet. Mol. Biol. Wortmann,André Castagna; Simon,Daniel; Mazzoleni,Luiz Edmundo; Sander,Guilherme Becker; Francesconi,Carlos Fernando de Magalhães; Nabinger,Débora Dreher; Grott,Camila Schultz; Rech,Tássia Flores; Mazzoleni,Felipe; Lunge,Vagner Ricardo; Bona,Laura Renata de; Milbradt,Tobias Cancian; Silveira,Themis Reverbel da. Abstract Functional dyspepsia and lactose intolerance (adult-type hypolactasia, ATH) are common conditions that may coexist or even be confounded. Their clinical presentation can be similar, however, lactose intolerance does not form part of the diagnostic investigation of functional dyspepsia. Studies on the association between functional dyspepsia and ATH are scarce. This study aimed to evaluate whether ATH is associated with symptoms of functional dyspepsia. Patients fulfilling the Rome III diagnostic criteria for functional dyspepsia underwent genetic testing for ATH. Dyspeptic symptoms were evaluated and scored according to a validated questionnaire. The diagnostic criteria for ATH was a CC genotype for the -13910C/T polymorphism, located upstream of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bloating; Dyspepsia; Gastrointestinal diseases; Lactose intolerance; Single nucleotide polymorphism. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100092 Registros recuperados: 3 Primeira ... 1 ... Última

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