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Comparative two-dimensional gel electrophoresis maps for promastigotes of Leishmania amazonensis and Leishmania major BJID
Brobey,Reynolds K. B.; Mei,Fang C.; Cheng,Xiaodong; Soong,Lynn.
The outcome of Leishmania infections is determined by both the parasite species and the host genetic makeup. While much has been learned regarding immune responses to this parasite, our knowledge on parasite-derived factors is limited. The recent completion of the L. major and L. infantum genome sequence projects and concurrent advancement in proteomics technology would greatly accelerate the search for novel Leishmania proteins. Using a proteomics-based approach to study species-specific Leishmania proteins, we developed high-resolution, broad pH (3-10) two-dimensional gel electrophoresis (2-DE) separations to determine protein-expression profiles between highly infectious forms of the parasitic species L. amazonensis (New World) and L. major (Old World)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmania infections; Gel electrophoresis; Future studies.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000100001
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