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| Caron,Angelo Luis; Picanço-Castro,Virgínia; Ansorge,Sven; Covas,Dimas Tadeu; Kamen,Amine; Swiech,Kamilla. |
| ABSTRACT Lentiviral vector-mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost-effective manner. Here we describe a high yield production of a lentivirus encoding recombinant factor VIII in a scalable and GMP-compliant culture system, based on serum free suspension cultures and transient transfection with an inexpensive reagent, polyethylenimine (PEI), reaching a total viral yield of 2.48x108 particles. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Factor VIII; Hek293 cells; Lentiviral vectors; Polyethylenimine; Serum-free suspension culture; Transient transfection. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600923 |
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