|
|
|
|
|
Sardenne, Fany; Bodin, Nathalie; Metral, Luisa; Crottier, Anais; Le Grand, Fabienne; Bideau, Antoine; Brisset, Blandine; Bourjea, Jerome; Saraux, Claire; Bonhommeau, Sylvain; Kerzerho, Vincent; Bernard, Serge; Rouyer, Tristan. |
Various protocols are currently used to study marine lipids, but there is a growing interest in working on dry samples that are easier to transport. However, reference protocols are still lacking for dry samples. In order to make recommendations on this use, lipid classes and fatty acids (FA) obtained from six analytical protocols using two different tissue states (dry vs wet) and three extraction methods (automat vs manual potter vs leaving the solvent to work on tissue) were compared. Three dry storage modes of tissue (freezer vs gas nitrogen vs dry room) during one and three months were also compared. These comparisons were made on seven marine species with different lipid profiles, including fishes, crustaceans and mollusks. Lipid classes and FA... |
Tipo: Text |
Palavras-chave: Degradation; Freeze-drying; Lyophilization; Lipid class; Marine animal; Protocol. |
Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00467/57898/60189.pdf |
| |
|
|
Wold, P. -a.; Hoehne-reitan, K.; Cahu, Chantal; Zambonino, Jose-luis; Rainuzzo, J.; Kjorsvik, E.. |
The aim of the present study was to compare effects of dietary n-3 highly unsaturated fatty acids (HUFA) being incorporated in the phospholipid (PL) or in the neutral lipid (NL) fraction of the larval feed, on larval growth and histology of digestive organs in Atlantic cod (Gadus morhua L.) larvae. Three isoproteic and isolipidic diets, labelled according to the percentage of n-3 docosahexaenoic acid and eicosapentaenoic acid contained in NL1 or in PL1 and PL3 of the diets, were fed to cod larvae from 17 days post hatching (dph) to 45 dph. In the liver, hepatocytes and their nuclei were smaller in NL1 larvae compared with the PL larvae; the mitochondrial membrane structures were less dense and the amount of lipids observed in the liver was significantly... |
Tipo: Text |
Palavras-chave: Stereology; Phospholipids; Nutrition; Lipid class; Larvae; Histology; Diet; Cod. |
Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6777.pdf |
| |
|
|
CHIA,MATHIAS A.; LOMBARDI,ANA T.; MELãO,MARIA DA GRAÇA G.. |
The need for clean and low-cost algae production demands for investigations on algal physiological response under different growth conditions. In this research, we investigated the growth, biomass production and biochemical composition of Chlorella vulgaris using semi-continuous cultures employing three growth media (LC Oligo, Chu 10 and WC media). The highest cell density was obtained in LC Oligo, while the lowest in Chu medium. Chlorophyll a, carbohydrate and protein concentrations and yield were highest in Chu and LC Oligo media. Lipid class analysis showed that hydrocarbons (HC), sterol esthers (SE), free fatty acids (FFA), aliphatic alcohols (ALC), acetone mobile polar lipids (AMPL) and phospholipids (PL) concentrations and yields were highest in the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carbohydrates; Lipid class; Microalgae; Proteins. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000401427 |
| |
|
|
|