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| Fontana,Márcia Camponogara; Laureano,João Víctor; Forgearini,Betielli; Chaves,Paula dos Santos; Araujo,Bibiana Verlindo de; Beck,Ruy Carlos Ruver. |
| A specific, precise, and accurate LC-UV method was developed and validated to assay raloxifene hydrochloride in rat plasma. Raloxifene was analyzed after liquid-liquid extraction and quantified by reversed phase liquid chromatography (C18 column) using acetonitrile and ammonium acetate buffer 0.05 M (pH 4.0) as mobile phase at a flow rate of 1 mL.min-1 and UV detection at 287 nm. Retention times of raloxifene and internal standard (dexamethasone) were approximately 11 min and 14 min, respectively. Linearity was checked for a concentration range between 25 ng.mL-1 and 1000 ng.mL-1. Intra- and inter-day precision had relative standard deviation lower than 10% and 15%, respectively. Recovery from plasma was higher than 90%. Accuracy values were 98.21%,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Raloxifene/pharmacokinetic; Liquid chromatography.; Plasma.; Bioavailability.. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100538 |
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