SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Saraoui, Taous; Fall, Papa-abdoulaye; Leroi, Francoise; Antignac, Jean-philippe; Chereau, Sylvain; Pilet, Marie France.

Listeria monocytogenes is a pathogenic Gram positive bacterium and the etiologic agent of listeriosis, a severe food-borne disease. Lactococcus piscium CNCM I-4031 has the capacity to prevent the growth of L. monocytogenes in contaminated peeled and cooked shrimp. To investigate the inhibititory mechanism, a chemically defined medium (MSMA) based on shrimp composition and reproducing the inhibition observed in shrimp was developed. In co-culture at 26°C, L. monocytogenes was reduced by 3-4 log CFU g-1 after 24 h. We have demonstrated that the inhibition was not due to secretion of extracellular antimicrobial compounds as bacteriocins, organic acids and hydrogen peroxide. Global metabolomic fingerprints of these strains in pure culture were assessed by...

Tipo: Text

Palavras-chave: Listeria monocytogenes; Lactococcus piscium; Nutrients competition; Cellular contact; Chemically defined medium; Bacterial interaction.

Ano: 2016

URL: https://archimer.ifremer.fr/doc/00250/36123/34677.pdf

High-Throughput Identification of Candidate Strains for Biopreservation by Using Bioluminescent Listeria monocytogenes

Applications of protective cultures, bacteriocins and bacteriophages in fresh seafood and seafood products

El Kheir, Sara M.; Cherrat, Lamia; Awussi, Ahoefa A.; Ramia, Nancy E.; Taha, Samir; Rahman, Abdur; Passerini, Delphine; Leroi, Francoise; Petit, Jeremy; Mangavel, Cecile; Revol-junelles, Anne-marie; Borges, Frederic.

This article describes a method for high-throughput competition assays using a bioluminescent strain of L. monocytogenes. This method is based on the use of the luminescent indicator strain L. monocytogenes EGDelux. The luminescence of this strain is correlated to growth, which make it suitable to monitor the growth of L. monocytogenes in mixed cultures. To this aim, luminescence kinetics were converted into a single numerical value, called the Luminescence Disturbance Indicator (LDI), which takes into account growth inhibition phenomena resulting in latency increase, decrease in the luminescence rate, or reduction of the maximum luminescence. The LDI allows to automatically and simultaneously handle multiple competition assays which are required for...

Tipo: Text

Palavras-chave: Biopreservation; Listeria monocytogenes; High-throughput screening assays; Bioluminescence; Competition; Mixed culture; Bioprotection; Antibacterial activities.

Ano: 2018

URL: https://archimer.ifremer.fr/doc/00454/56534/58236.pdf

Pilet, Marie-france; Leroi, Francoise.

Microbial seafood-borne disease represents 10 to 20% of the total food-borne outbreaks. Most of them are from bacterial origin and involve seafood products that have been contaminated by pathogenic Vibrio, Listeria monocytogenes and histaminogen bacteria. On the other hand, seafood products are very sensitive to the development of spoiling bacteria producing off-odours. Pathogenic and spoiling microflora are not always reduced or limited by the processing steps that are currently used in these foodstuffs, and the interest for alternative techniques such as bioprotection to improve quality and safety of seafood has increased in the last years. Among the microbial flora of lightly preserved seafood products, lactic acid bacteria usually become dominant...

Tipo: Text

Palavras-chave: Biopreservation of seafood; Seafood safety and spoilage; Lactic acid bacteria; Sensory quality; Listeria monocytogenes.

Ano: 2010

URL: http://archimer.ifremer.fr/doc/00030/14138/11384.pdf

Biodiversity of Listeria monocytogenes sensitivity to bacteriocin-producing Carnobacterium strains and application in sterile cold-smoked salmon

Adaptative response of Listeria monocylogenes to osmotic and chill stress: consequences on food safety.

Brillet, Anne; Pilet, M; Prevost, H; Bouttefroy, A; Leroi, Francoise.

The aim of this study was to demonstrate the inhibitory capacity of Carnobacterium strains against a collection of Listeria monocytogenes strains in cold-smoked salmon (CSS). Methods and Results: Three bacteriocin-producing strains, Carnobacterium divergens V41, C. piscicola V1 and C. piscicola SF668, were screened for their antilisterial activity against a collection of 57 L. monocytogenes strains selected from the French smoked salmon industry, using an agar spot test. All the Listeria strains were inhibited but three different groups could be distinguished differing in sensitivity to the three Carnobacterium strains. However, C. divergens V41 always had the highest inhibitory effect. The antilisterial capacity was then tested in sterile CSS blocks...

Tipo: Text

Palavras-chave: Listeria monocytogenes; Cold smoked salmon; Carnobacterium; Biopreservation; Bacteriocin.

Ano: 2004

URL: http://archimer.ifremer.fr/doc/2004/publication-610.pdf

Improving simultaneously the quality and safety of cooked and peeled shrimp using a cocktail of bioprotective lactic acid bacteria

Elmnasser, Noura; Ritz Bricaud, Magali; Guillou, Sandrine; Leroi, Francoise; Orange, Nicole; Bakhrouf, Amira; Federighi, Michel.

Listeria monocytogenes is it psychrotrophic food-borne pathogen. This organism is problematic for food production industry due to its ubiquitous distribution ill nature and its tolerance to condition of osmotic and chill stress. One common strategy employed by many bacteria and by L. monocytogenes for adaptation to osmotic stress is by the intracellular accumulation of compatible solutes. Two different types of adaptative reponses to low temperature have undertaken by L. monocytogenes. One of the responses is accomplished by adjusting membrane fluidity through alteration of the membrane fatty acid composition. A second response consists ill the accumulation of osmolytes. In this brief review we will focus on these general systems Which are Of particular...

Tipo: Text

Palavras-chave: Food safety; Chill stres; Osmotic stress; Adaptation; Listeria monocytogenes; Sécurité des aliments; Stress froid; Stress salin; Adaptation; Listeria monocytogenes.

Ano: 2006

URL: http://archimer.ifremer.fr/doc/2006/publication-2549.pdf

Saraoui, Taous; Cornet, Josiane; Guillouet, Emilie; Pilet, Marie France; Chevalier, Frederique; Joffraud, Jean-jacques; Leroi, Francoise.

Tropical shrimp is of considerable economic importance in the word but is highly perishable due to microbial and chemical degradation. Biopreservation is a food preservation technology based on the addition of “positive” bacteria able to kill or prevent the growth of undesirable microorganisms. Two strains of lactic acid bacteria (LAB) have previously been selected for a biopreservation strategy: Lactococcus piscium CNCM I-4031, for its ability to prevent the sensory deterioration of seafood and Carnobacterium divergens V41, which inhibits growth of Listeria monocytogenes. The objective was to test the association of the two strains to improve both the quality and safety of shrimp. In a first trial, the two LAB were inoculated alone or in a cocktail in...

Tipo: Text

Palavras-chave: Carnobacterium divergens V41; Lactococcus piscium CNCM I-4031; Penaeus vannamei; Listeria monocytogenes; Biopreservation.

Ano: 2017

URL: https://archimer.ifremer.fr/doc/00353/46412/46140.pdf

Bioprotective Effect of Lactococcus piscium CNCM I-4031 Against Listeria monocytogenes Growth and Virulence

Saraoui, Taous; Leroi, Francoise; Chevalier, Frederique; Cappelier, Jean-michel; Passerini, Delphine; Pilet, Marie-france.

Listeria monocytogenes is a Gram-positive pathogen occurring in many refrigerated ready-to-eat foods. It is responsible for foodborne listeriosis, a rare but severe disease with a high mortality rate (20–30%). Lactococcus piscium CNCM I-4031 has the capacity to prevent the growth of L. monocytogenes in contaminated peeled and cooked shrimp and in a chemically defined medium using a cell-to-cell contact-dependent mechanism. To characterize this inhibition further, the effect of L. piscium was tested on a collection of 42 L. monocytogenes strains. All strains were inhibited but had different sensitivities. The effect of the initial concentration of the protective and the target bacteria revealed that the inhibition always occurred when L. piscium had reached...

Tipo: Text

Palavras-chave: Biopreservation; Lactococcus piscium; Listeria monocytogenes; Co-culture; Cell ratio; Scanning electron microscopy; Virulence.

Ano: 2018

URL: https://archimer.ifremer.fr/doc/00449/56065/57588.pdf

Response of Listeria monocytogenes to liquid smoke

Sélection et caractérisation de souches de Carnobacterium pour la biopréservation du saumon fumé

Guilbaud, Morgan; Chafsey, Ingrid; Pilet, Marie-france; Leroi, Francoise; Prevost, Herve; Hebraud, Michel; Dousset, Xavier.

Aims: To investigate the effect of liquid smoke on growth, survival, proteomic pattern and haemolytic potential of Listeria monocytogenes. Methods and Results: Growth and survival curves were recorded in brain-heart infusion broth supplemented with three concentrations of liquid smoke. L. monocytogenes growth was inhibited in the presence of 15 mu g ml(-1) phenol while a rapid decrease in cell viability occurred in the presence of 30 mu g ml(-1) phenol. The proteome of L. monocytogenes cytosoluble proteins was slightly modified after 2-h incubation with 30 mu g ml(-1) phenol but no protein already characterized in response to other known stresses was induced, except the protease ClpP. Liquid smoke inhibited the haemolytic potential without affecting hly...

Tipo: Text

Palavras-chave: Survival; Stress; Proteome analysis; Listeria monocytogenes; Liquid smoke; Haemolysis.

Ano: 2008

URL: http://archimer.ifremer.fr/doc/2008/publication-4522.pdf

Molecular characterization of Listeria monocytogenes isolated from foods

Brillet, Anne.

In order to develop a biopreservation strategy in cold-smoked salmon, the inhibitory capacity of 3 bacteriocin-producing Carnobacterium strains isolated from fish have been tested towards Listeria monocytogenes strains. C. divergens V41 maintains the level of Listeria below 50 CFU/g in sterile cold-smoked salmon during the 28 days of vacuum storage at 4 and 8°C, thanks to the divercin V41 action. For its use in the product, the culture of the strain was optimized in a growth medium without animal protein. On commercial cold-smoked salmon slices, the strain inhibits slightly the endogenous microflora and doesn't modify the sensorial quality, but it produces tyramine, a biogenic amine. A non-producing tyramine mutant was obtained by chemical mutagenesis....

Tipo: Text

Palavras-chave: Tyrosine decarboxylase; Tyramine; Cold smoked salmon; Listeria monocytogenes; Divercin V41; Carnobacterium; Biopreservation; Tyrosine décarboxylase; Tyramine; Saumon fumé; Listeria monocytogenes; Divercine V41; Carnobacterium; Biopréservation.

Ano: 2005

URL: http://archimer.ifremer.fr/doc/2005/these-2414.pdf

Rev. Microbiol.

Pimenta,Fabiana Cristina; Furlanetto,Sirdéia Maura Perrone; Mayer,Leonard W.; Timenetsky,Jorge; Santos,Manoel Armando Azevedo dos.

A total of 30 strains of Listeria monocytogenes isolated from different foods (16 of differents kinds of sausage, 14 cheese,) purchased at groceries of São Paulo City were ribotyped and analysed for the presence and expression of hemolysin gene and production of phosphatidylinositol-specific phosphalipase C - PI-PLC enzyme. The L. monocytogenes strains were differentiated into six ribotype classes. A total of 13 (43.3%) from these strains belong to the same ribotype (ribotype I), and was coincident to the ribotype of the standard L. monocytogenes prototype strain (ATCC-15313). The hemolytic activity was observed in 29 (96.7%) strains when incubated at 37oC, but not at 4oC. The direct colony hybridization method for hemolysin gene detection showed a...

Cepas de Lactobacillus spp. con capacidades probióticas aisladas del tracto intestinal de terneros neonatos

Palavras-chave: Listeria monocytogenes; Ribotyping; Virulence; Factors; Food.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400011

Rev Salud Anim.

Sánchez,Lilian; Omura,Michele; Lucas,Adams; Pérez,Tania; Llanes,Marisleidys; Ferreira,Celia de Luce.

Se caracterizaron in vitro 17 cepas de Lactobacillus spp. aisladas del tracto intestinal de terneros neonatos, para seleccionar las mejores como candidatos probióticos y realizarles posteriormente los estudios in vivo. A todos los aislados se les realizó la prueba de la catalasa, la tinción de Gram y se estudió sus características culturales para su identificación preliminar. Se evaluó su capacidad probiótica mediante pruebas in vitro como fueron el crecimiento a diferentes temperaturas (30, 37 y 45°C), pH (2,5; 3,4; 5,4; 6,4) y capacidad de acidificación del medio de cultivo. Se preseleccionaron 10 cepas de Lactobacillus spp. que crecieron en todas las condiciones evaluadas. Se destaca el crecimiento de células viables de 6,7-10 log UFC/ml que...

Tipo: Journal article

Palavras-chave: Lactobacillus spp.; Probiótico; Salmonella sp.; Staphylococcus aureus; Escherichia coli; Listeria monocytogenes.

Ano: 2015

URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2015000200004

Ocorrência de Listeria monocytogenes em queijo artesanal tipo coalho comercializado na cidade de Manaus-AM, Brasil

Acta Amazonica

Ramos,Simone de Nazaré Melo; Costa,Cristóvão Alves da.

A ocorrência de Listeria sp. em queijo artesanal tipo coalho comercializado na cidade de Manaus foi estudada. No período de março a maio de 1998 foram coletadas 58 amostras do produto em feiras e mercados localizados em seis zonas da cidade de Manaus. Em duas amostras de queijo foram identificadas duas espécies de Listeria: L. monocytogenes e L. innocua. A primeira espécie é reconhecidamente patogênica para o ser humano. Os resultados apresentados demonstraram haver risco de transmissão de enfermidades pelo consumo de queijos processados sem os devidos cuidados de higiene.

Actividad antagonista de bacterias lácticas aisladas del medio marino contra cepas de Listeria

Palavras-chave: Queijo; Listeria monocytogenes; Listeria sp.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672003000400007

ABCL

Marguet,Emilio Rogelio; Vallejo,Marisol; Sierralta Chichisola,Verónica; Quispe,Jorge León.

En el presente trabajo se estudiaron 74 bacterias lácticas para determinar su capacidad de inhibir el desarrollo de Listeria monocytogenes ATCC 7644 o L. innocua ATCC 33090. Dentro de las 7 que exhibieron actividad, 2 se seleccionaron para estudios complementarios y se identificaron por métodos fenotípicos y genotípicos como Enterococcus mundtii Tw 56 y Lactococcus lactis subsp. lactis Tw 34. Después de la neutralización con NaOH y tratamiento térmico (100 ºC y 121 ºC durante 5 min) la actividad antagonista del sobrenadante libre de células se mantuvo activa. El tratamiento con lisozima, lipasa y catalasa no afectó la actividad antimicrobiana. Sin embargo, la actividad enzimática de la tripsina suprimió la acción inhibitoria del sobrenadante, confirmando...

URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572011000200008

Palavras-chave: Bacterias lácticas; Listeria monocytogenes; Bacteriocinas; Ambiente marino.

Ano: 2011

Listeriose em rebanho de ovinos leiteiros na região serrana do Rio Grande do Sul: relato de caso

Ribeiro,L.A.O.; Rodrigues,N.C.; Fallavena,L.C.B.; Oliveira,S.J.; Brito,M.A..

Relatam-se dois casos de listeriose ocorridos em ovinos leiteiros da raça Lacaune, mantidos em confinamento e alimentados com silagem de milho. O exame histológico do tecido nervoso mostrou meningoencefalite supurada, e a imunoistoquímica foi positiva para Listeria monocytogenes com isolamento da bactéria do tecido nervoso. Enfatiza-se a ocorrência dessa enfermidade em ovinos leiteiros que apresentavam lesões na cavidade oral e eram alimentados com silagem de baixa qualidade.

Antimicrobial activity of the Nisin Z producer Lactococcus lactis subsp. lactis Lc08 against Listeria monocytogenes in skim milk

Palavras-chave: Ovino; Listeria monocytogenes; Listeriose.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000300005

Perin,L.M.; Miranda,R.O.; Camargo,A.C.; Colombo,M.; Carvalho,A.F.; Nero,L.A..

The presented study aimed to verify the effect of different pH values, enzyme solutions and heat treatments on the antimicrobial activity of the bacteriocinogenic strain Lactococcus lactis subsp. lactis Lc08 and to test their antimicrobial activity against Listeria monocytogenes in reconstituted skim milk at refrigeration temperatures. This strain was previously described as a nisin Z producer and capable of inhibiting L. monocytogenes growth in in vitro tests. The antimicrobial activity of the bacteriocin cell-free supernatant of Lc08 was sensitive to enzyme treatments (except papain). The pH values and heating (65ºC for 30min, 75ºC for 15s) had no apparent effect on the antimicrobial activity of the bacteriocin produced by Lc08. Only treatment at...

Atividade antimicrobiana de bactérias lácticas de embutidos curados frente a Listeria monocytogenes

Palavras-chave: Nisin Z; Milk; Antagonistic activity; Lactococcus lactis subsp. lactis; Listeria monocytogenes.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000500037

Prado,C.S.; Santos,W.L.M.; Carvalho,C.R.; Moreira,E.C.; Costa,O..

Cepas de bactérias lácticas recuperadas de 336 colônias isoladas e selecionadas foram submetidas ao teste de atividade antimicrobiana direta, que identificou as produtoras de substâncias antimicrobianas capazes de inibir in vitro o desenvolvimento de duas cepas indicadoras de Listeria monocytogenes. As 108 cepas que inibiram diretamente pelo menos uma das cepas indicadoras receberam a denominação DTEI e foram selecionadas para o teste de atividade antimicrobiana indireta contra as mesmas cepas de L. monocytogenes, assim como frente a outras cepas de bactérias lácticas de origens diversas. Essa atividade inibidora indireta foi avaliada por meio de sobrenadantes isentos de células, esterilizados por meio de microfiltração, eliminando-se os principais...

Occurrence of Listeria monocytogenes in silages assessed by fluorescent in situ hybridization

Palavras-chave: Bactéria láctica; Atividade antimicrobiana; Listeria monocytogenes; Lactobacillus sp.

Ano: 2000

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400022

Oliveira,M.; Guerra,M.; Bernardo,F..

Avaliou-se a qualidade microbiológica da silagem produzida em Portugal e otimizaram-se alguns aspectos relacionados com a eficácia de detecção de Listeria monocytogenes mediante protocolo baseado na técnica de hibridação in situ fluorescente (FISH) para detecção directa desse microrganismo em amostras de silagens. O protocolo foi aplicado a 74 amostras, em simultâneo com o método bacteriológico convencional. Este último permitiu a detecção de L. monocytogenes em 11 silos (15%). Por meio do protocolo FISH, observou-se que 22 silos (29,7%) se encontravam contaminados. O método FISH apresentou precisão (77,0%), especificidade (92,5%) e sensibilidade (72,7%) elevadas, sendo adequado para a análise microbiológica presuntiva de silagens, uma vez que é um método...

Influence of the cleaning system of conveyor belts on microbiological quality of poultry meat

Palavras-chave: Silage; Listeriosis; Fluorescent in situ hybridization; Listeria monocytogenes; Portugal.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000100038

Julião,T.A.; Cortezi,A.M.; Rossi,G.A.M.; D’Abreu,L.F.; Balieiro,J.C.C.; Vidal,A.M.C..

ABSTRACT This study focused on assessing the microbiology of conveyor belts surfaces, cleaned or not with pressurized water, and verification of the influence of this process on the microbiological quality of poultry meat. A reduction on mesophilic, psychrotrophic and Enterobacteriaceae counts (P<0.05) on dry conveyor belts compared to wet ones was observed. For the chicken leg (consisting of drumstick and thigh) samples, no statistically significant differences were detected on counts of indicators of microorganisms on poultry legs (composed by drumstick and thigh). For poultry meat conducted on wet or dry conveyor belts, 99% and 86%, were positive for Listeria spp, respectively. Only one sample of chicken leg was positive for presence of L....

Effect of eugenol on growth and listeriolysin o production by Listeria monocytogenes

Palavras-chave: Enterobacteriaceae; Listeria monocytogenes; Food microbiology; Staphylococcus spp..

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401325

BABT

Filgueiras,Cristina Tostes; Vanetti,Maria Cristina Dantas.

The inhibitory effect of eugenol, a naturally occurring compound mainly present in the essential oil fraction of cloves, was studied on the growth and listeriolysin O (LLO) production by Listeria monocytogenes. Potassium efflux from cells promoted by eugenol was also determined after 24 h incubation in phosphate buffered saline. Eugenol promoted a delay on the growth of L. monocytogenes at concentrations of 100, 300 and 500 µg mL-1and above 800 µg mL-1 the effect was bactericidal. Production of LLO by L. monocytogenes in the presence of eugenol was reduced 80-100%. An accumulation of external K+ was observed above 300 µg mL-1 of eugenol which indicated that the cell membrane was affected. The results showed the effectiveness of eugenol in controlling...

Virulence genes and genetic relationship of L. monocytogenes isolated from human and food sources in Brazil

Palavras-chave: Eugenol; Listeria monocytogenes; Antilisteric; LLO.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400008

BJID

Almeida,Rosana Macedo de; Barbosa,André Victor; Lisbôa,Rodrigo de Castro; Santos,André Felipe das Mercês; Hofer,Ernesto; Vallim,Deyse Christina; Hofer,Cristina Barroso.

ABSTRACT The herein presented assay provided a bacteriological and molecular characterization of 100 samples of L. monocytogenes isolated from human (43) and food (57) sources, from several regions of Brazil, and collected between 1975 and 2013. Antigenic characterization defined 49% of serotype 4b samples, followed by 28% of serotype 1/2b, 14% of serotype 1/2c, 8% of serotype 1/2a, and 1% of serotype 3b. Both type of samples from human and food origin express the same serotype distribution. Multiplex PCR analysis showed 13 strains of type 4b with the amplification profile 4b-VI (Variant I). Virulence genes hly, inlA, inlB, inlC, inlJ, actA, plcA, and prfA were detected in all samples, highlighting a deletion of 105pb on the actA gene in 23% of serotype 4b...