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| Registros recuperados: 60 | |
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| Sánchez-Castrejón,Edna; Ponce-Rivas,Elizabeth; Aguilar,Manuel B; Díaz,Fernando. |
| Crustacean hyperglycemic hormone (CHH) is the most abundant and best studied member of the CHH/MIH/GIH neuropeptide hormone family. CHH plays a major role in controlling glucose levels in the hemolymph, and it also has significance in regulating molting, reproduction, and osmoregulation. In contrast, molt-inhibiting hormone (MIH) is responsible for maintaining animals in an intermolt stage. In this study, Liv-MIH-1 cDNA, which encodes a mature neuropeptide from the eyestalk of white shrimp, Litopenaeus vannamei, was expressed in methylotrophic yeast (Pichia pastoris KM71) under the control of an alcohol oxidase promoter. Recombinant Liv-MIH-1 was secreted into the culture medium using the α-factor prepro-sequence without Glu-Ala repeats. The... |
| Tipo: Journal article |
Palavras-chave: CHH; Litopenaeus vannamei; Pichia pastoris; Recombinant protein. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400009 |
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| Gonçalves,Michelle Mantovani; Lemos,Manoel Victor Franco; Galetti Junior,Pedro Manoel; Freitas,Patrícia Domingues de; Furtado Neto,Manuel Antonio Andrade. |
| The Pacific white shrimp, Litopenaeus vannamei (Penaeidae), represents about 95% of all Brazilian shrimp production. The Brazilian L. vannamei foundation broodstock was made up of specimens collected from different American Pacific sites, but little information was collected on the genetic structure of the broodstock. We used the fluorescence amplified fragment length polymorphism (fAFLP) method to study the genetic diversity of L. vannamei broodstock lines 03CMF1 and 03CBF1 originally produced by breeder-shrimps imported mainly from Panama and Ecuador, although wild individuals from other localities may also have been used in producing these two lines. Our results showed a total of 93 polymorphic bands ranging from 50 to 500 bp, the mean Nei's genetic... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: FAFLP; Genetic diversity; Litopenaeus vannamei; Penaeidae; Shrimp. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200016 |
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| De Donato,Marcos; Ramirez,Raúl; Howell,Chris; Verginelli,Roberto; Orta,Tomás; Cabrera,Saúl; Mata,Enrique; Manrique,Ramón. |
| We investigated the effects of family selection on the growth rates of four genetic lines of Litopenaeus vannamei, the Pacific white shrimp, from Venezuela. The mean family weight at selection was 16.79 g and the mean growth rate was 0.169 g d-1 at a mean age of 138 days. The mean growth rates per generation were 0.141 g d-1 for the parental generation (P0), 0.173 g d-1 for the F1 and 0.191 g d-1 for the F2. Survival varied from 83.3% to 94.4%. There were statistically significant differences between lines, with lines B and C, growing at higher temperatures and lower salinities, showing the fastest growth. Regression analysis between growth rate and age for each genetic line showed high r² values, but smaller than those shown between growth rate and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Family selection; Genetic improvement; Growth rate; Litopenaeus vannamei. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500009 |
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| PEREIRA, A. M. L.; SILVA, A. E. M. da; OLIVEIRA, C. R. do R.; SILVA, D. A. da; OLIVEIRA, D. W. S; SANTOS, E. P. dos; GONÇALVES JUNIOR, G. F.; KATO, H. C. de A.; ABREU, J. L. de; SILVA, L. O. B. da; PIMENTEL, O. A. L. F.; LIMA, P. C. M. de; OLIVEIRA, V. Q. de; GÁLVEZ, A. O.. |
| Introdução. Ciclo do nitrogênio na carcinicultura. Microrganismos responsáveis pela oxidação e transformação dos compostos nitrogenados. Estratégias de fertilização com carbono orgânico para o controle dos compostos nitrogenados no cultivo de camarão marinho. Estratégias de utilização do carbono inorgânico para auxiliar o controle dos compostos nitrogenados no cultivo de camarão marinho. Qualidade de água e sua importância para ajudar no controle dos compostos nitrogenados. Fertilização mineral em sistemas de baixa salinidade e sua influência sobre os compostos nitrogenados. Considerações finais. Referências. |
| Tipo: Livros |
Palavras-chave: Camarão marinho; BRS Aqua; Aquicultura; Cadeia Produtiva; Ciclo do Nitrogênio; Viveiro; Carcinicultura; Qualidade da Água; Aquaculture; Nitrogen cycle; Food chain; Nursery management; Shrimp; Nitrogen compounds; Litopenaeus vannamei; Water quality. |
| Ano: 2022 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1144229 |
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| Labreuche, Yannick; Veloso, Artur; De La Vega, Enrique; Gross, Paul S.; Chapman, Robert W.; Browdy, Craig L.; Warr, Gregory W.. |
| Many questions remain unanswered regarding RNAi-based mechanisms and dsRNA-induced antiviral immune responses in penaeid shrimp. In this study, we report the characterization in the white leg shrimp Litopenaeus vannamei of RNAi pathway associated proteins Lv-Ago 1 and Lv-Ago 2, two members of the Argonaute family of proteins, as well as Lv-sid 1, the first shrimp homologue of Sid-1, a membrane channel-forming protein implicated in the cellular import of dsRNA. To decipher their functional implication in RNAi-related phenomena, we monitored their relative expression following stimulation by specific and non-specific RNA duplexes of diverse length. The findings show that the length of small RNA duplexes plays a critical role in the activation of both... |
| Tipo: Text |
Palavras-chave: Shrimp; Litopenaeus vannamei; RNAi; DsRNA; Argonaute; Sid-1; Antiviral immunity. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00015/12652/9587.pdf |
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| Gamboa Delgado, Julián; Molina Poveda, César; Cahu, Chantal. |
| A study was conducted to evaluate variations of digestive enzyme activities in Litopenaeus vannamei (Boone) reared in commercial ponds under semi-intensive conditions. Shrimp were collected at each body weight increase of 2 g. As the shrimp grew (2-12 g), significant increases in the activities of lipase and chymotrypsin were observed. The total protease activity decreased from 6 g onwards. Trypsin activity showed a peak at 6 g and amylase activity increased two-fold after 2 g. Additionally, the stomach contents were analysed microscopically for shrimp between 2 and 10 g. Plant matter contributed above 30% of the total stomach content in 6-, 8- and 10-g shrimp. Detritus represented 58% and 62% of the total stomach content in 2- and 4-g shrimp,... |
| Tipo: Text |
Palavras-chave: Semi intensive culture; Stomach content; Digestive enzymes; Litopenaeus vannamei; Shrimp. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-902.pdf |
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| Li, Erchao; Arena, Leticia; Lizama, Gabriel; Gaxiola, Gabriela; Cuzon, Gerard; Rosas, Carlos; Chen, Liqiao; Van Wormhoudt, Alain. |
| Improvement in the osmoregulation capacity via nutritional supplies is vitally important in shrimp aquaculture. The effects of dietary protein levels on the osmoregulation capacity of the Pacific white shrimp (L. vannamei) were investigated. This involved an examination of growth performance, glutamate dehydrogenase (GDH) and Na+-K+ ATPase mRNA expression,, and GDH activity in muscles and gills. Three experimental diets were formulated, containing 25%, 40%, and 50% dietary protein, and fed to the shrimp at a salinity of 25. After 20 days, no significant difference was observed in weight gain, though GDH and Na+-K+ ATPase gene expression and GDH activity increased with higher dietary protein levels. Subsequently, shrimp fed diets with 25% and 50% dietary... |
| Tipo: Text |
Palavras-chave: Litopenaeus vannamei; Gene expression; Glutamate dehydrogenase; Na+-K+ ATPase; Protein; Salinity. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00031/14261/11692.pdf |
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| Aguirre Guzman, G; Ascencio, F; Saulnier, Denis. |
| The pathogenicity of Vibrio penaeicida Strains KH-1 and AM101, their culture-free supernatant (CFS), and their protein fraction obtained by 40% of ammonium sulfate precipitation (PFs40) were assessed in experimental challenges against juvenile Litopenaeus vannamei. Live Vibrio cells, CFS, and PFs40 from the AM101 strain produced a significantly higher mortality (p < 0.05) compared to the KH-1 strain. Toxicity and median lethal doses (LD50) of Fast Protein Liquid Chromatography (FPLC) products were evaluated on L. vannamei. The first FPLC fraction sample (A) from PFs40 of the AM101 strain displayed LD50 values of 1.68 and 5.61 mu g protein ind.(-1), respectively. The second FPLC process from Fraction A showed a peak (A1) also with toxic effects to... |
| Tipo: Text |
Palavras-chave: Pathogenicity; Exotoxin; Shrimp; Litopenaeus vannamei; Cysteine protease; Vibrio penaeicida. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-3618.pdf |
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| Comoglio, Li; Gaxiola, G; Roque, A; Cuzon, Gerard; Amin, O. |
| Juveniles of the white shrimp Litopenaeus vannamei were kept Without food for between 0 to 15 clays to evaluate the impact of starvation oil physiologic state (oxygen consumption, poststarvation refeeding, index, nitrogen excretion, and O:N ratio) and digestive enzymes activity. Physiologic changes were found after 6 days of fasting, and refeeding ability declined as a result. Nevertheless. the shrimp were able to Survive 16 days Without food. Starvation Caused metabolism to drop progressively toward a basal level (21 J (.) h(-1 .) g(-1)) and a decrease in the rate of ammonia excretion, because of the catabolism of amino acids front soluble protein in the hepatopancreas. This decrease led to an increase in digestive enzymes specific activity (U/mg... |
| Tipo: Text |
Palavras-chave: Digestive enzymes; Litopenaeus vannamei; Metabolic rate; Shrimp; Starvation. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/00000/10877/7911.pdf |
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| Chavez-calvillo, G.; Perez-rueda, E.; Lizama, G.; Zuniga Aguilar, J. J.; Gaxiola, G.; Cuzon, Gerard; Arena-ortiz, L.. |
| We evaluated changes in gene expression of Litopenaeus vannamei shrimp in response to a change in diet by comparing specimens which were fed with an animal protein diet against those which underwent a change from an animal protein diet to a plant protein diet. In addition, we also compared specimens which were fed with a plant protein diet with another group which underwent a change from plant to animal protein diet. Results showed that, in the case of hepatopancreas tissue, a total of six genes were differentially expressed and exhibited a high degree of homology with sequences which codify for proteins related to metabolism, transcription regulation, and rRNA subunits. In the case of muscle tissue, four differentially expressed sequences were recorded... |
| Tipo: Text |
Palavras-chave: Differential display; RT-PCR; Litopenaeus vannamei; Protein diet; Alternative protein sources. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00002/11298/7853.pdf |
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| Galindo, Carmen; Gaxiola, Gabriela; Cuzon, Gerard; Chiappa-carrara, Xavier. |
| Biochemical changes, specifically in hemocyanin and glucose concentrations in hemolymph, glycogen in the digestive gland. were studied throughout the molt cycle in juveniles of Litopenaeus vannamei in a high salinity condition (36), and related to variations in the osmotic capacity of organisms. Increasing hemolymph volume before molting modifies circulating glucose and hemocyanin concentrations acting on the osmotic capacity. Variations in glucose concentrations are indicators of differential energy requirements throughout the molt cycle. Hemocyanin was used as an indirect indicator of the energy consumed by shrimp in various molt processes that affect the osmotic capacity. The co-variability of these metabolites is the result of the biochemical... |
| Tipo: Text |
Palavras-chave: Molt cycle; Litopenaeus vannamei; Hypo osmotic conditions; Biochemistry. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6899.pdf |
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| Hipolito,M; Catroxo,M. H. B; Martins,A. M. C. R. P. F; Melo,N.A; Pituco,E. M; Galleti,N.T.C; Ranzani-Paiva,M. J. T; Mouriño,J.L.P; Ferreira,C.M. |
| In this study thirty shrimp samples from commercial marine shrimp (L. vannamei) farms of southern region of Brazil were obtained. Hepatopancreas and shell scrapings fragments collected in these animals were processed by transmission electron microscopy using negative staining (rapid preparation), immunoelectron microscopy and immunocytochemistry (immunolabelling with colloidal gold particles) techniques. On the transmission electron microscopy a great number of white spot virus particles, ovoid or bacilliform-to-ellipsoid, measured 230-290 nm in length and 80-160 nm in diameter with intra-nuclear projections were visualized by the negative staining technique in 27 (90%) out of 30 samples examined. Using immunoelectron microscopy technique, the anti-VP 664... |
| Tipo: Journal article |
Palavras-chave: White spot syndrome virus; Negative staining; Immunoelectron microscopy; Immunocytochemistry; Marine shrimp; Litopenaeus vannamei. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200067 |
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| Santos,Daniele Bezerra dos; Freire,Fúlvio Aurélio de Morais; Pontes,Cibele Soares. |
| O objetivo deste trabalho foi avaliar o efeito do tipo de substrato sobre a preferência, o enterramento e a atividade natatória de camarões (Litopenaeus vannamei). Juvenis (0,93±0,29 g) e adultos machos e fêmeas (10,05±1,18 g) foram observados durante 24 horas, por meio do método animal focal, com registros instantâneos. Foram testadas as seguintes granulometrias (tratamentos) dos substratos: I, 100% C; II, 50% C + 50% B; III, 50% A + 50% C; IV, 100% B; V, 50% B + 50% A; e VI, 100% A, em que as letras referemse ao diâmetro médio das partículas, A = 0,25-2,0 mm; B = 0,0625-0,25 mm; e C = <0,0625 mm. Foram aplicados seis tratamentos com 18 repetições para cada categoria: camarões juvenis, machos e fêmeas. Cada indivíduo foi exposto por 24 horas aos seis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Litopenaeus vannamei; Bem-estar animal; Carcinicultura; Etologia aplicada. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000800008 |
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| Guertler,Cristhiane; Rieg,Tailin; Mejía-Ruíz,Claudio Humberto; Lehmann,Maurício; Barracco,Margherita Anna; Perazzolo,Luciane Maria. |
| O objetivo deste trabalho foi avaliar a proteção antiviral específica via RNA de interferência (RNAi) contra o vírus da síndrome da mancha-branca (WSSV), em camarões marinhos (Litopenaeus vannamei). Os camarões foram injetados com uma sequência dsRNA específica (vp28 do envelope viral), seguida por desafio com WSSV após 48 horas. Avaliaram-se o hemograma às 0, 3, 6, 24, 48 e 72 horas após o desafio, e a taxa de mortalidade durante 30 dias. Nos animais tratados com dsRNA vp28, a infecção viral foi limitada, e a sobrevivência (73%) e a "clearance" viral (80%) foram maiores do que nos camarões infectados, não tratados, que apresentaram 100% de mortalidade em cinco dias. Nos camarões tratados com dsRNA, o hemograma diminuiu até 6 horas após o desafio, seguido... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Litopenaeus vannamei; Defesas antivirais; Hemócitos totais; Imunologia de crustáceos; Vírus da mancha-branca. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000800028 |
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| Mello Junior,Carlos Cesar de; Delsol,Gael Yvan Leclercq; Motte,Emmerik; Escobar,Virna Alexia Cedeño; Rey,Pedro Filipe; Martins,Mauricio Laterça; Arana,Luis Alejandro Vinatea; Mello,Giovanni Lemos de; Farias,Alvaro Pestana de; Arguello,Xavier Antonio Serrano; Maridueña,John Erick Montaño. |
| The objective of this work was to select surviving breeders of Litopenaeus vannamei from white spot syndrome virus (WSSV) outbreak, adapted to local climatic conditions and negatively diagnosed for WSSV and infectious hypodermal and hematopoietic necrosis virus (IHHNV), and to evaluate if this strategy is a viable alternative for production in Santa Catarina, Brazil. A total of 800 males and 800 females were phenotypically selected in a farm pond. Nested-PCR analyses of 487 sexually mature females and 231 sexually mature males showed that 63% of the females and 55% of the males were infected with IHHNV. Animals free of IHHNV were tested for WSSV, and those considered double negative were used for breeding. The post-larvae produced were stocked in nine... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Litopenaeus vannamei; Breeding; Epidemiology; Nested-PCR. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000500011 |
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| Registros recuperados: 60 | |
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