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Nutritional regulation and tissue specificity of gene expression for proteins involved in hepatic glucose metabolism in rainbow trout (Oncorhynchus mykiss) ArchiMer
Panserat, Stéphane; Plagnes Juan, E; Kaushik, Sadasivam.
Rainbow trout (Oncorhynchus mykiss) are known to use dietary carbohydrates poorly. One of the hypotheses to explain the poor utilisation of dietary glucose by these fish is a dysfunction in nutritional regulation of hepatic glucose metabolism. In this study, we obtained partial clones of rainbow trout cDNAs coding for a glucose transporter (Glut2), and for the enzymes 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (6PF-2K/F-2,6BPase), fructose-1,6-bisphosphatase (FBPase) and pyruvate kinase (PI(), Their deduced amino acid sequences were highly similar to those of mammals (up to 80% similarity), In a study of nutritional regulation, the Glut2 gene was highly expressed in the liver irrespective of the nutritional status of the trout, in agreement with...
Tipo: Text Palavras-chave: Oncorhynchus mykiss; Trout; Bifunctional enzyme; Pyruvate kinase; Fructose bisphosphatase; Glucose transporter; Glucose metabolism; Liver metabolism.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-415.pdf
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Sunflower protein concentrate and crambe protein concentrate in diets for silver catfish Rhamdia quelen (Quoy and Gaimard, 1824): use as sustainable ingredients Anais da ABC (AABC)
LOVATTO,NAGLEZI M.; LOUREIRO,BRUNO B.; PIANESSO,DIRLEISE; ADORIAN,TAIDA J.; GOULART,FERNANDA R.; SPERONI,CAROLINE S.; BENDER,ANA BETINE B.; MÜLLER,JUCIELI; SILVA,LEILA P. DA.
Abstract The objective of this study was to evaluate growth and metabolic parameters of silver catfish fed with protein concentrates of sunflower meal (SMPC) and crambe meal (CrMPC). The study evaluated two levels of substitution, where 25 or 50% of animal protein was replaced with plant-based protein. A total of 300 silver catfish (14 ± 0.26 g) were used in five treatments and three replications, in fifteen 280-liter experimental units. The results were submitted to analysis of variance and the means of the control diet was compared to the remaining treatments by Dunnett’s test at 5% significance level. At the end of the trial, no differences were observed for the variables final weight and daily weight gain. However, minor feed conversion was observed in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Crambe abyssinica; Fish feeding; Helianthus annus; Jundiá; Liver metabolism; Plant-based protein concentrates.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000703781
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Responsiveness of glycogen breakdown to cyclic AMP in perfused liver from rats with insulin-induced hypoglycemia BJMBR
Vardanega-Peicher,M.; Curi,R.; Pagliarini e Silva,S.; Nascimento,K.F.; Bazotte,R.B..
The responsiveness of glycogen breakdown to cAMP was investigated in isolated perfused liver from male Wistar fed rats (200-220 g) with insulin-induced hypoglycemia. The activation of glycogenolysis by 3 µM cAMP was decreased (P<0.05) in livers from rats with hypoglycemia induced by the administration of insulin or during the direct infusion of insulin into the isolated liver. The direct effect of insulin on glycogen catabolism promoted by 3 µM cAMP occurred as early as 3 min after starting insulin infusion. In contrast, the cAMP agonists resistant to phosphodiesterases, 8Br-cAMP and 6MB-cAMP, used at the same concentration as cAMP, i.e., 3 µM, did not modify the effect of insulin. The data suggest that the decreased hepatic responsiveness of glycogen...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insulin-induced hypoglycemia; Cyclic AMP; Glycogen breakdown; Liver metabolism.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100007
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