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In vitro apprach to the study of chronic exposure to low does of X-rays JBAG
Ponzinibbio,M.V; Peral-García,P; Seoane,A.
The present research was undertaken in order to study the effect of repeated low doses of radiation on two different cell lines from the same species. An in vitro model test was developed to avoid the influence of the confounding factors affecting epidemiological studies and to simulate a chronic exposure (50 mSv of x-rays during ten consecutive days). Comet assay and early apoptosis were analyzed immediately after exposure and after chronic irradiation. Sequential irradiation induced an increase of cells showing DNA damage. Index Damage was higher than that of the controls in both cell lines immediately after exposure and after chronic irradiation. These differences between exposed and control cells were statistically significant only for the transformed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apoptosis; Comet assay; In vitro; Ionizing radiation; Low dose.
Ano: 2011 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332011000200004
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Ethanol in low chronic dose level attenuates major organic effects in malnourished rats Biol. Res.
AGUIAR,ALINE S DE; BOAVENTURA,GILSON T; ABRAHÃO,RAFAEL F; FREITAS,THATIANA L; TAKIYA,CHRISTINA M; FILHO,PORPHIRIO J S; SILVA,VILMA A DA.
The aim of the present study was to evaluate the chronic toxicity of ethanol low blood levels in malnourished rats. Female Wistar rats (220 g) were subjected to either an ad libitum diet (W, well-nourished, n=10) or food restriction (M, malnourished, n=10). Water (WW and MW) or ethanol solution (W5% and M5%) was offered to half of each nutritional group (n=5) as the only fluid source. The treatment was continued for two months. After sacrifice, blood biochemical parameters and macroscopic, histologic and morphometric evaluation of the liver were performed. Results indicated that: Ethanol consumption was higher in malnourished rats and minimized body weight loss in malnourished rats, while it decreased the body weight gain in well-nourished ones. Behavioral...
Tipo: Journal article Palavras-chave: Ethanol; Low dose; Malnutrition; Toxicity.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100004
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