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Effects of low-intensity pulsed ultrasound exposure on rats tibia periosteum Anais da ABC (AABC)
Batista,Jaqueline Martins; Nakagaki,Wilson Romero; Soares,Evelise Aline; Camilli,José Angelo.
Abstract The periosteum is a rich source of osteoprogenitor cells and periosteal grafts can be used as an alternative method to replace bone grafts. The low-intensity pulsed ultrasound (LIPUS) has often been used as a noninvasive method to stimulate osteogenesis and reduce the fracture healing time. The aim of this study was to evaluate the effects of the ultrasound exposure on the rat tibia periosteum. Group I (7 animals) received LIPUS therapy on the left tibia for 7 days and group II (7 animals) on the left tibia for 14 days. After euthanasia, the tibias were processed. Number of periosteal cells and vessels and thickness of the periosteum were analyzed. The number of periosteal cells was higher in stimulated periosteum compared to controls at 7 and 14...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tibia; Blood vessels; Periosteal cells; Periosteum; Low-intensity pulsed ultrasound.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100901
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Low-intensity pulsed ultrasound promotes proliferation and migration of HaCaT keratinocytes through the PI3K/AKT and JNK pathways BJMBR
Leng,Xiaoyan; Shang,Jing; Gao,Danhui; Wu,Jiang.
Although the effects of low-intensity pulsed ultrasound (LIPUS) on diverse cell types have been fully studied, the functional role of LIPUS in keratinocytes remains poorly understood. This study aimed to investigate the effects of LIPUS on proliferation and migration of HaCaT cells as well as the regulatory mechanisms associated with signaling pathways. Human HaCaT cells were exposed or not to LIPUS, and cell proliferation and migration were measured by BrdU incorporation assay and Transwell assay, respectively. Expression of proteins associated with proliferation and migration was evaluated by western blot analysis. Expression of key kinases in the PI3K/AKT and JNK pathways was also evaluated by western blot analysis. Effects of LIPUS on the PI3K/AKT and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Low-intensity pulsed ultrasound; HaCaT cells; Proliferation; Migration; PI3K/AKT/JNK.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200603
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