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Dissecting the Opaque-2 regulatory network using transcriptome and proteome approaches along with enzyme activity measurements Scientia Agricola
Lefèvre,Agnès; Consoli,Luciano; Gaziola,Salete A.; Pellegrino,Ana Paula; Azevedo,Ricardo A.; Damerval,Catherine.
The Opaque-2 (O2) gene encodes a transcriptional activator specifically expressed for grain development of maize. o2 mutants have an opaque and chalky kernel, with a decrease in zein storage protein content, and an increase in the proportions of lysine and tryptophan. In this review, we present recent results investigating genetic properties of the O2 network, using transcriptome and proteome approaches, associated with measurements of activities of enzymes of the aspartate pathway and lysine degradation. The structural polymorphism at the O2 locus was investigated by RFLP in a collection of 51 maize inbred lines. Most polymorphic sites were found outside the coding regions. We then searched for relationships between RFLP polymorphism and (i) mRNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zein; Aspartate kinase; Lysine oxoglutarate reductase/saccharopine dehydrogenase; Polymorphism.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000200031
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